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Daphnia Heart Rate

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My group recorded higher heart rate compare to the class average when we used caffeine on the Daphnia as seen in Figure 2. Several errors could have accounted for this data. This including perhaps the Daphnia’s heart rate may have changed because of fear while being moved from the bowl to the microscope slide. Another possible error could have also been the amount of time that it took to change the solutions, which could have accounted for slight variations in heart rate. Temperature is yet a further error to account for as the microscope slide temperature was constantly changing to view the specimen, and change the dosage of caffeine or alcohol. The temperature of the solutions could also have played a factor in the Daphnia Heart rate as ideal conditions for Daphnia is 68 degrees Fahrenheit and the temperature of the solutions was not measured for. …show more content…

The results that we concluded were the same with what we expected from our hypothesis. The Daphnia heart rate increased higher as it was placed in the various solutions of caffeine. The heart rate had a decrease as the Daphnia was exposed to the alcohol. This experiment helps understand how cellular biology works in a controlled environment. We also learn and understand the effects of alcohol and caffeine does to our body and health. Not just human but animals and other organisms as well. Daphnia biology still showed a reaction even though we tried the experiment on a smaller scale a larger scale. Daphnia biology showed the same reaction the exact way a human would when exposed to a stimulant or

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A variety of factors can change the heart rate of Daphnia. According to Richard Handy (2012), “The heart rate (which can be up to 300 beats per minute) can be monitored and counted in different conditions – for example changing water temperature, or changing the type

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Corotto, F., Ceballos, D., Lee, A., & Vinson, L. (2010). Making the Most of the Daphnia Heart Rate Lab: Optimizing the Use of Ethanol, Nicotine & Caffeine. The American Biology Teacher , 176-179. The purpose of this article was to test the effects of chemicals on Daphnia magna.

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During this experiment, mitochondria were isolated from 20.2 grams of cauliflower using extraction buffer, filtration through Miracloth, and centrifusion. Twelve samples containing various volumes of mitochondrial suspension, assay buffer, DCIP, sodium azide, and citric acid cycle intermediates were prepared to be read by a spectrophotometer. The inclusion of the dye DCIP allowed for the absorbance of the reactions between the mitochondrial suspension and the TCA cycle intermediates succinate, malonate, and oxalate to be measured, as DCIP turns from blue to colorless as the activity of succinate dehydrogenase increases. Experimental Findings Increasing the number of mitochondria in the reaction did increase the reduction of DCIP relative to the amount of mitochondrial suspension present.

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Introduction Metabolism is the sum of all anabolic and catabolic reactions within a living organism to sustain life. The energy required to perform these reactions is provided by oxygen in the form of ATP, therefore the oxygen consumption rate can be measured to determine the metabolic rate. Since oxygen is obtained through respiration, the efficiency of an organism’s respiratory system affects its metabolism. Previous studies have shown that caffeine affects the human respiratory center and occasionally dilates bronchus. It can thus stimulate human respiration and increase the metabolic rate (Haggins et al, 1915).

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Metabolism The daphnia’s main composition consists of a midgut, hindgut and an esophagus running from its head down its body, using one compound eye as an aid in swimming. The position of the heart is right above where the location of eggs in the females back. The water flea’s usual food source is

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The body will naturally venture to dilute the heavy intake of sugar and caffeine, but without the balance throughout the body, then the body begins to fail. This is an issue that we have to take

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