As we known that the cell represents the structural and functional unit in living organisms, human health of the health of its cells and that any defect in the work of the cell, if not cured by the body, lead to the occurrence of diseases may be deadly, such as cancer. To ensure this consistency, the cell contains a set of components that work in an orderly manner that allows the production of material necessary for cell survival and repair any errors .
The Living organisms are classified according to their cell components into two large families: Prokaryote, organisms that are in the cell with a single cell and are simple in composition. The second type is eukaryote, organisms with more complex cells.
Both types of families must continue to produce proteins and enzymes (a type of protein that works to facilitate some chemical reactions) in order to obtain energy, build membranes, defend the cell and other activities. Because of the multiplicity of functions, one cell contains thousands of types of proteins. But the duration of the activity of a single protein does not last forever. Its structure undergoes change and decay after it has been involved in the various
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Means that if we want to identify the active genes in the cell, we can take all the proteins in the cell and see who is the protein in the largest proportion. This method seems logical, but from the practical side, there are several challenges. Firstly, the structure of the protein is different from the structure of the gene and its analysis is still difficult. Therefore, the analysis of thousands of proteins will take considerable time, and that one amino acid can be encoded by several codons, Sequence of amino acids in the protein We will have several probabilities for the gene formula that produced this