Microsatellite Primers

Development of SSR Markers in Satureja bachtiarica , based on 454 Sequencing

Premise of the study: Microsatellite primers were developed for Satureja bachtiarica (Lamiaceae) an endemic medicinal plant of Iran. to reveal genetic characteristics in wild populations.
Methods and Results: Using next-generation sequencing, 454-GS-FLX, 15 microsatellite markers were identified, of which 11 were identified as polymorphic. These markers were study on 60 individuals from five populations of S.bachtiarica. The number of alleles per locus ranged from two to 9, with the observed heterozygosity ranging from 0.159 to 0.882.
Conclusions: :This study shows the effectiveness of SSR markers in used to detect polymorphism between S.bachtiarica populations which

(savory) belongs to the Lamiaceae family with 200 which include 30 species, is primarily distributed in Europe, West Asia, North Africa and Mediterranean basin extended to Irano-Turanian geographical regions ( Momtaz and Ablollahi, 2010. Sefidkon and Jamzad 2005; Rechinger 1982). As literature reveals, 16 species are endemic of Iran that, naturally occur in mountainous of western and northern parts in Iran. Satureja bachtiarica Bunge is a highly valued medicinal plant that among the endemic species (Rechinger, 1982, Saeidnia et al, 2010). It usually grow in Mountainous areas of north, northwest, northeast, central and south-western Iran, distribution and grow on rocky limestone cliffs or slopes. The main chemical constituents of essential oil are aromatic monoterpenes which are responsible for characteristic odor and taste (Chizzola., 2003), which thymol and carvacrol phenolic compound main, among them. The various genius of savory in the difference countries has been considered Because of pharmacologic properties and use in traditional medicine. Because of they are widely known efficacy for treating many health problems, the harvest of wild populations has been greatly expanded and wild resources of this species are currently undergoing a rapid

2 demonstrated the statistics in the SSR loci detected. Of the SSR loci, dinucleotides accounted for 77 % and thus ranked the most abundant, followed by trinucleotides (21 %), hexanucleotides and, tetranucleotides (2 %). The total number of alleles per locus observed across the five populations ranged from four to 17 (table 2). The 11 polymorphic SSR markers detected a total of 118 alleles in the five populations of S.bachtiarica. All markers yielded different numbers of alleles in the all populations. In total, almost the maximum number of alleles per locus S.bach17 was observed in all groups. Because of the high mean number of alleles per microsatellite markers, it is appropriate to estimate the genetic diversity (Powell et al. 1996) therefor markers with the higher number alles is proper to assess genetic variation. He for each locus was generally, ranging from 0.166 (Locus9) to 1 (Locus1 and Locus10) and Ho ranged from 0.159 (Locus9) to 0.8826 (Locus6). All 11 loci were considered moderately to highly informative and, therefore, adequate for genetic studies. A total of six loci (S.bach01, S.bach08, S.bach17, S.bach23, S.bach24, and S.bach28) significantly deviated from HWE (P < 0.05) at least one of the