Digestion Lab Report

In the experiments regarding digestive processes both chemically and physically, the main focus was on the digestion of carbohydrates, proteins and fats and the use of artificial processes to demonstrate the breakdown of each. Experiment one had two parts that tested for starch as well as reducing sugars. This experiment was done by mixing various measurements of water, starch and amylase and allowing some tubes to boil for minutes at a time. When all tubes were incubated and ready, the IKI and Benedict’s test had to be run on the tubes testing negative for starch as well as detecting no reducing sugars. Experiment two testing for proteins through the use of various mixtures of water, trypsin and BAPNA. A few of these tubes were boiled but

Mechanical digestion begins the process in the mouth by chewing the food and chemical digestion takes over and allows for the breakdown of food into smaller nutrients with the help of saliva. The breakdown of food is possible through enzymes that allow for absorption by the body. Enzymes usually contain amylase and lipase. Both of these enzymes were used throughout the experiments. Saliva contains an enzyme known as amylase that helps break down carbohydrates. Carbohydrates such as starch as broken down by amylase into smaller molecules known as monosaccharides. These molecules, once digested, are absorbed into the small intestine. Enzymes also break down proteins into amino acids and are moved to the gastrointestinal tract where they are absorbed. Lipase functions to break down lipids into fatty acids. These fatty acids are digested into the small

My hypothesis for this experiment was that test tube 1A would test positive for starch and negative for reducing sugars. Test tube 2A would test negative for starch and negative for reducing sugars. Test tube 3A would test negative for both starch and reducing sugars. I also thought test tube 4A and 5A would test positive for starch as well as positive for reducing sugars. Test tube 1A contained 3 drops of water and 3 drops of starch. Test tube 2A contained 3 drops of water mixed with 3 drops of amylase. Test tube 3A combined 3 drops of water with 3 drops of maltose. Test tube 4A boiled 3 drops of amylase for 10 minutes and then added 3 drop of starch. Test tube 5A was the only tube that mixed 3 drops starch with 3 drops amylase.
The second experiment involved the digestion of proteins into amino acids through the use of trypsin. The main purpose of this experiment was to test for BAPNA hydrolysis. My hypothesis was for test tube 1T, 3 drops of water mixed with 3 drops of trypsin, to be negative for BAPNA hydrolysis. Test tube 2A, 3 drops of water and 3 drops BAPNA, to be positive for hydrolysis. Test tube 3A, 3 drops of trypsin boiled for 10 minutes and mixed with 1 mL BAPNA, to test positive for hydrolysis. Test tubes 4T and 5T, 3 drops of BAPNA and 3 drops trypsin, to be positive for