The sugar used in this experiment was created by mixing ten milliliters of starch,glucose, and regular countertop sugar. This was transferred into the joint water bottle tunnel using a funnel. Both ends of the tunnel were sealed shut and each measurements were taken every three minutes and final measurements were taken after 21 minutes. The results were
Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable pH 3. Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity 1.
Additionally, it was difficult obtaining a piece of rhubarb that was thin and particularly red, therefore the effect could not be best observed in the cells. Part B: Design your own experiment Parts of this practical were taken and slightly altered from the following link http://www.markedbyteachers.com/gcse/science/investigate-the-effect-of-surface-area-on-osmosis-in-potato-tissue.html Aim: To observe the effect different surface area: volume ratios have on osmosis in potato tissue. Hypothesis: If the potato has a larger surface area: volume ratio, the quicker osmosis will take place and the larger the mass will be at the end of the experiment, therefore the difference in mass of the potatoes from the start of the experiment to the end of the experiment will be larger. Additionally, the potato pieces left in a saltwater solution will decrease in mass, whereas the pieces left in water will increase in mass.
7. In this experiment, if the sucrose concentration were increased to 70 g/l would you expect sucrase activity to be significantly higher than the activity at 35 g/l. Explain your answer. No, because based on the results once it reached 30 g/l 35 g/l the results had stayed the same. There, the activity is lessening and coming to what looks like a plateau. 8.
Regulating the osmolarity can be accomplished by balancing the concentration of sodium ions with the correct volume of water. Sodium is the main ion that affects the osmolarity of extracellular fluids. The regulation of osmolarity must be assimilated with the regulation of water volume as any changes to one will affect the other. When you need more body fluids you will lose more water than sodium and the osmolarity of the body fluids will increase. The body must keep a certain amount of water however a large amount of sodium can be allowed to be lost.
Negative: Water Constant: Iodine solution (indicator) Hypothesis: At least two out of the three procedures will be indicated as starch, due to the fact that amylase is the first enzyme that breaks down carbohydrates Materials: • Paper • Pen/pencil • Saliva • Water • Hydrochloric acid • Pipette • Iodine solution • Construction paper • Timer Procedure: 1. A sheet of paper was
We then took the potato cores out of the empty beaker and dabbed them lightly with paper towel to get any excess solution off. We did this quickly and following it we then took the mass of all four potato cores again and recorded
Osmosis is the special movement of water, generally in a cell. In this experiment, the cell is represented by the egg in the center of the beaker. The concept of osmosis is water moving through a cell membrane without energy, and flowing with the concentration gradient. Osmosis
Diffusion and Osmosis Lab Report By: Jettica Williams BIOL 1107 Lab September 21, 2016 Prepared for Mrs. Fulford Lab Course Page Break The cell membrane act as a roadblock for cells. The cell membrane has a very hectic job. It restricts the access to what comes in and what goes out. The bond the membrane shares with others is the idea of accountability.
For this lab I will be using water and sucrose to demonstrate the rate of osmosis. In this lab I will be exploring how temperature impacts the rate of osmosis by placing pieces of potato of equal size in solutions of different temperatures and observing the change in mass of potato after a given period of time. The change in mass will indicate the rate of osmosis.
Our Hypothesis stated in the introduction of the paper was correct. The potato slices in the sucrose solution lost weight (grams), while the potato slices in the water gained weight (also in grams). Osmosis definitely occurred during this experiment. In one short hour changes can be seen between the weights of the potatoes. The skins on the potatoes are made of cellulose, membrane which does not allow osmosis to occur, or water to pass through under normal circumstances.
By using the same mass of potato slices and putting them in different concentration of solutions for a specific amount of time will tell us how the concentration changes the mass of the potato slice. Therefore changing the rate of osmosis. Hypothesis: I predict that, if the piece of potato was put into a solution that has a high concretion of sucrose then the potato slice would lose mass as it would lose water from its cells because the water is moving out of the cell from a high concentration to a low concentration of water through a semi- permeable membrane. The cell is hypotonic and the solution is hypertonic.
Uncontrolled Environmental conditions Atmospheric conditions The controlled variable Concentration of amylase was kept under control by measuring the amount of amylase used and also it was made sure the percentage of amylase used was 1%. The Amount of amylase/starch used were kept to 5cm3 at all times. Materials needed Beakers Bunsen burner Test tube Thermometer Stopwatch Test plate Glass rod Starch Amylase solution Water bath Iodine solution. Test tube holder Labels Marker Procedure First 5 test tubes were taken and labeled with numbers from 1 to
The percentage weight change is dependent on surface area of potato Controlled The amount of salt solution used Percentage of salt in solution Length of time the potato is sat in water Materials needed 3 Potatoes 600 ml 15% salt solution Utensils 4x 300 ml beaker Knife Cutting board Scale Ruler Procedure 1. 8 rectangular prisms will be cut from the potatoes;
In conducting the experiment, the researcher used gloves and cloth to extract the blended squash in order to come up with a better result. The time frame for the study is for 7 days. The study was therefore conducted in the the JICA Laboratory in the month of January.