ipl-logo

E. Coli Transformation Lab Report

506 Words3 Pages

Amir Ahemedin Ms.Buckley Genetics 11/06/15 Transformation of E.coli Lab Purpose The purpose of this lab is to genetically engineer the E.coli strain by introducing two genes, the green fluorescent protein gene (GFP) and the ampicillin resistant gene (AMP). Then observe whether or not the E.Coli strain would take up these genes and become fluorescent. Background Information In this lab, bacterial transformation was one of the three processes that occurred when genetic material is introduced to a bacterial cell. Bacterial transformation is important because it allows for the cloning and movement of DNA between strains. This transformation usually occurs within plasmids, which are closed circular molecules made up of double stranded DNA. The function of the plasmid is to provide bacteria with genetic advantages such as antibiotic resistance. In this lab, the plasmids provided the ampicillin resistance and the fluorescence. If the bacterial cells are grown in the presence of the antibiotic ampicillin then only the cells that took up the plasmid have the resistance gene. As a result the resistance gene will have to keep the plasmid and the GFP gene. The other thing that plasmids …show more content…

Many scientist today cut portions of DNA with restriction enzymes and include a piece of new genetic material into the bacterial cells. The only way this transformation can take place is if the bacteria is competent and is grown to the right stage in which they are dividing the most. In order for this to happen,one would treat the bacterial cells with Calcium Chloride (CaCl2). The bacteria used in this lab was E.Coli and it was an ideal bacteria because it can be easily grown on agar. Hypothesis The transformed E.Coli with the ampicillin resistance gene will be able to grow in the ampicillin plates and it would have a green glowing color.

Show More
Related

Tn 4351 Unit 5 Lab Report

506 Words | 3 Pages

chinesis. A construct of R751::Tn4351 (the physical map of R751::Tn4351 and restriction sites are shown in fig. 7) was selected for introduction into F. chinesis to discover if the introduction and insertion of the vector R751 and the transposition of T4351 into the F. chinesis chromosome by a triparental mating occurred. One parent was E. coli GJ342 which carried a helper plasmid, the second parent was E. coli HB101 which contained R751::Tn4351 and the third parent was the F. chinesis target strain. 189 colonies were isolated on LB agar plates which in passage in fresh media were able to grow in 200µgml-1 erythromycin.

Read More

Bacillus Megaterium Lab Report

914 Words | 4 Pages

Gram positive organisms are susceptible to antibiotics such as penicillin (Introduction to Penicillins, 2012). This is because, gram positive bacteria have a thick layer of peptidoglycan, a sugar and peptide coating that gives a cell its shape and helps it stay intact (Wiley, 2004). The original antibiotic, penicillin, and the likes are used to treat infections caused by bacteria that are gram positive. Based on the scope of the experiment and the materials needed, a budget estimate of $1,000.00 would be needed to buy reagents and materials for this experiment.

Read More

Unit 2 Plasmid Research Paper

735 Words | 3 Pages

For a plasmid to be useful as a recombinant DNA vector, it must have some essential features. What

Read More

Intercellular Gene Transfer Lab Report

1100 Words | 5 Pages

Figure 3. Testing of transformed and mutant bacteria on minimal medium Growth was observed on the Transformed (Trsf) section and not on the Mutant (Mut)

Read More

Pglo Transformation Lab Report

664 Words | 3 Pages

The hypothesis for this experiment was that transformed bacterial cells would grow on ampicillin plates and glow green when exposed to UV light. The rationale for this hypothesis was because the plasmid would code for ampicillin resistance and a green fluorescent protein, we would have the outcome explained in the hypothesis. 4. Our predictions were that for the standard protocol plates with agar and ampicillin, there would be growth and the colonies would glow under UV light. On the modified protocol on plates with agar and ampicillin where we changed the time of the heat shock, we expected less growth but the colonies would still glow.

Read More

Unknown Bacteria Lab Report

1034 Words | 5 Pages

Introduction Our world is composed of many bacteria’s’ that can either help or destroy us. Therefore, its’s imperative to learn and study them. The purpose of the lab was to put into action the methods that have been learned in the laboratory to determine our unknown bacteria. Bacteria’s can have different features, shapes, and or arrangements that help microbiologist determined their role in our life (whether they are good or bad for humans).

Read More

Colistin Case Study

2242 Words | 9 Pages

Afterwards, with the use of Glimmer 3.02 and BLAST, gene prediction and annotation were completed with another plasmid, pHN122-1, as a reference. To confirm the role of the gene that caused polymyxin resistance and contained mcr-1, the gene and its sequence were place into a cloning vector pUC18 that yielded pUC18-mcr-1. With this yield and electroporation, it was used to transform an E.coli strain, revealing its ability to confer colistin resistance. Q3D:

Read More

Bio 14 Lab Report

819 Words | 4 Pages

In playing with these particular conditions, I found that some conditions could make both strains coexist relatively well in the antibiotic region of the simulation. This experiment is so scientifically interesting in that the results of this experiment should tell us with whether high-mutation rates or low mutation rates impart a competitive advantage to a certain strain in an antibiotic environment. This be applied to predict which strains of bacteria are more likely to thrive when new antibiotics are developed. With the question found, an experimental design was developed to address this question.

Read More

Kirby Bauer Diffusion Test Lab Report

624 Words | 3 Pages

INTRODUCTION: In this experiment I was testing for antimicrobial sensitivity of Staphylococcus epidermidis by using the Kirby-Bauer Diffusion test. The three antibiotics utilized in this lab were: gentamicin, novobiocin, and penicillin. I determined the effectiveness of the antibiotic by observing and measuring the zone of inhibition for each antibiotic.

Read More

Bethany Brookshire

906 Words | 4 Pages

It could have also been helpful to show a diagram of how the cell transfers the genetic information containing antibiotic resistance characteristics.

Read More

Antibiotic Streptomycin Lab Report

815 Words | 4 Pages

Introduction: This experiment includes the use of a genome editing system in order to make E.coli cells resistant to the antibiotic streptomycin. The system uses a protein called Cas9. Guide RNA is used to guide the Cas9 to the targeted sequence of DNA. The Cas9 finds the match to the gRNA in the cell’s DNA and cuts it out. As the cell tries to repair the DNA, it uses the template DNA that was inserted into the cell, and results in the modified DNA.

Read More

E Coli Meditation Lab Report

806 Words | 4 Pages

In this experiment we investigated how a high or low mutation rate of E. Coli will affect its growth in a lactose medium and whether different concentrations of each of the strains would produce different results. We hypothesized that a higher mutation rate would result in more lactose digestion among the E. Coli cultures and that a higher concentration of either strain would produce more lactose digesting cells, and normal unmutated cells as well. As previously predicted, the results below show that the greater the plated concentration of E. Coli, the more mutated lactose digesting cells there were after a week of growth. However, one thing of note is that the low mutator plates especially the 10^3 concentration, produced more similar growth

Read More

Shaver Fitness Evolution

856 Words | 4 Pages

It showed that when the frequency of a mutator clone is intentionally increased above a threshold, the mutator will spread further in association with the new beneficial mutations. When the frequency is below the threshold, the mutator clone will usually decline in the frequency. This is because the wild type clone is more commonly associated with new beneficial mutations. Research has shown that mutator hitchhiking in natural bacterial populations is implied by the large amount of frequencies of mutator strains in some natural

Read More

E. Coli Research Paper

1004 Words | 5 Pages

Escherichia Coli 0157: H7 This paper will specialize on a specific type of bacterial foodborne illness caused by the bacteria Escherichia Coli. E. coli was discovered by Theodore von Escherich in 1885. E.coli is a natural found bacteria that lies throughout the intestinal tract of warm blooded animals and comes in many forms only one of which is deadly. This form is E. coli 0157:H7 which can be caused by direct exposure to fecal matter to kill this rouge

Read More

Horizontal Evolution And Vertical Evolution Of Antibiotic Resistance

409 Words | 2 Pages

The AR is also conferred by the resistance mechanism and the conditions under which resistance is measured. Bacterial fitness is also affected using increasing concentrations of antibiotic. The existence of genes in bacterial genomes that generate resistance by itself is intrinsic resistance. Many bacteria have this resistance [15]. Gene pickup, heterologous expression, HGT, and mutation are associated with resistance development

Read More

More about E. Coli Transformation Lab Report

Open Document