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Enzyme Catalase Lab Report

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Recommended: Catalase and enzyme activity

The Purpose To test and analyze the effect of pH and temperature on the reaction rate of the enzyme catalase. HYPOTHESIS The more base you add to the liver the reaction will be faster The more heated the liver is the longer it will take the reaction to take place than if the liver is cold or at room temperature, MATERIALS 8 test tubes Safety goggles Test tube rack Water Ice Bunsen burner Liver Hydrogen Peroxide (H2O2) Hydrochloric acid (HCL) Sodium Hydroxide (NaOH) Liver(cut into small pieces) Test tube holder 2 beakers Pipette Stirring rods Paper Towels Beaker stand Method Gather all the material and put on the safety goggles. Place a 1 piece of liver in 6 test tubes. Add water to all the 4 test tubes. Then take 1 beaker and fill it with water, and then place it over it over the bunsen burner on the beaker stand. …show more content…

Label the test tubes with hot Using a test tube holder put the test tubes into the beakers. Place 2 test tubes with the hot label in the hot water and the other 2 with the cold label in the ice. Let the test tubes be in the beaker for 5 minutes. Take all the test tubes out of the beaker and drain the water. Then add 3 ml of hydrogen peroxide using a pipette to all the 4 test tubes. Rate the reaction from 1-5 after adding the H2O2 in the test tubes. Take 2 new test tubes and put 3 ml of H2O2 in them using a pipette. Then add 10 drops of HCL into the 2 test tubes and put the liver piece in RESULTS Trial 1 Trial

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