ipl-logo

Lab Report Organic Compound Catecholase

805 Words4 Pages

Introduction The organic compound catecholase is commonly found in plants and consists of two substrates called catechol and oxygen. When these substrates react with each other they form a brown color, often seen in vegetables and fruits left out after being cut open. (Wahlert and Holland, 1999) This reaction is why when a potato is cut open and left to sit, it will begin to brown. During the genetic mutation of potatoes, scientists can limit the amount of the substrate which causes less severe browning or no browning in potatoes. (Adams and Brown, 2007). The potato contains enzymes in which the catechol reacts with and it speeds the process up. If catecholase were left alone and without a catalyst (enzyme) the reaction would take much longer. …show more content…

The control tube is tube number three because it contained the same ingredients as that of the first experiment where we determined that when catechol reacts with potato extract it turns brown. In test tubes one and two a different amount of catechol was added. This is because we needed to determine what kind of inhibitor the PTU was. Because there was no change in color with either test tube one or two, we found that PTU was a non-competitive inhibitor. Discussion The hypothesis created at the beginning of this lab was that PTU is a non-competitive inhibitor and the color of the solution would remain constant after the inhibitor was added due to it changing the shape of the enzyme completely and not allowing the catechol substrate to react with the potato enzyme. During the lab we found that PTU was a non-competitive inhibitor, due to no change in the color of the solution after the inhibitor was added. Our results were conclusive with our hypothesis but our hypothesis was incorrect as were our lab results. According to other studies done on the inhibitor phenylthiourea, it is in fact a competitive inhibitor and our experiment was incorrectly tested. The addition of the extra catechol was supposed to have sped up the reaction with the enzyme and force the inhibitor out of the enzymes active site. If there was more time allowed to observe any possible color changes the results would have been more conclusive and our results more accurate. In other

Show More
Related

Ph Levels Lab Hypothesis

421 Words | 2 Pages

The purpose of this project is to further test the effect of pH levels, and how they affect the production of enzymes. The hypothesis for this experiment was the more basic the ph buffer the high the activity of the enzyme. The more acidic the pH buffer the less activity of the enzyme. The first time the experiment was done with only three pH levels that were tested. That helped find the range of pH levels that were needed to test the second time.

Read More

Catechol Oxidase Lab Report

542 Words | 3 Pages

The substrate for the sweet potato catechol oxidase is N-phenylthiourea. In comparison to the possible substrates for catechol they both have hydrogen acceptor sites that can be used to hydrogen bond in the active site of the enzyme. Along with hydrogen bonding there may be some ionic bonding between some metal atoms in the active site of the enzyme and the substituents coming off of the benzene ring.

Read More

Catechol Oxidase Lab Report

642 Words | 3 Pages

Catechol oxidase is found in cell cytoplasm, their function in plants are to "help protect damaged plants bacterial and fungal disease." The objective of this experiment is to test the presences of catechol oxidase in various fruits and vegetables. Our group hypothesis states that, If catechol oxidase is present in the selected extracts, the null hypothesis is that catechol oxidase is not present in the selected extracts. Next, the prediction would be, if catechol oxidase doesn't differ with other enzyme sources, then the rates will

Read More

Bacterial Amylase Temperature

615 Words | 3 Pages

At last, at 95°C, or 85ºC, the enzyme activity is showed at being in its greater temperature. From black at 0 minutes, the color became bright yellow at 10 minutes, which specify the optimal temperature for

Read More

B-Galactosidase Lab Report

1764 Words | 8 Pages

Enzymes speed up chemical reactions enabling more products to be formed within a shorter span of time. Enzymes are fragile and easily disrupted by heat or other mild treatment. Studying the effect of temperature and substrate concentration on enzyme concentration allows better understanding of optimum conditions which enzymes can function. An example of an enzyme catalyzed reaction is enzymatic hydrolysis of an artificial substrate, o-Nitrophenylgalactoside (ONPG) used in place of lactose. Upon hydrolysis by B-galactosidase, a yellow colored compound o-Nitrophenol (ONP) is formed.

Read More

Catalase Reaction Lab Report

199 Words | 1 Pages

You then test your unknown organism for catalase. Label the last third of the MSA Plate used in Exercise 26 Unknown. Touch an isolated colony on the blood agar plate with a sterilized, cooled loop and transfer a tiny amount

Read More

Beta Galactosidase Lab Report

494 Words | 2 Pages

The competitive inhibitor that was added was lactose. We predicted this because competitive inhibitors block and bind to the active site so it will slow down the binding of the desired substrate. An alternative hypothesis that came up was that the reaction of substrate would stay consistent as if no inhibitor was added. The enzyme could reject the inhibitor if it does not fit in the active site, causing the substrate to bind as it normally would. Our results showed that with the addition of lactose, the reaction did slow down a considerably

Read More

Fair Test Lab Report

736 Words | 3 Pages

The method used was reliable and increased accuracy in the experiment, making it a fair test. This is proven throughout out the experiment because of many reasons such as the controlling the temperature. We did this by making sure the temperature of all enzymes tested, were consistent and had the same temperature, by putting the potato enzymes (Peroxidase) in a boiling tube then in a water bath to at a temperature of 20*c. This ensured a greater accuracy in the results, because if the temperature of some enzymes were higher than the rest, then the rate of reaction for those enzymes would have been faster as particles would move faster as they heat up speeding up the process. Our method was done at 20*c because this is close to room temperature

Read More

Mitochondrial Activity Lab Report

2102 Words | 9 Pages

First, it was hypothesized that test tube "A", the control, would not show any red concentration, test tube "B" which contains supernatant II would show the most red concentration and test tube "C" which contains sediment II would only show a little red concentration. The second hypothesis states that the raw corn kernels would have mitochondrial activity while the boiled corn kernels would not. The last hypothesis interprets that the "gunk" and sediment I will both contain starch granules. It was only expected to find mitochondrial activity in Supernatant II. Unfortunately, after performing this experiment, we were not able to support this hypothesis and come up with a conclusion.

Read More

Chromatography Lab Report

1393 Words | 6 Pages

Materials and Methods: For the chromatographic separation of plant pigments, pieces of spinach were ground with acetone to produce a watery extract. A line of extract was applied 1 cm from the bottom of a strip of chromatography paper. The line dried and the extract was reapplied. Once that dried, the paper was placed into a jar containing a small amount chromatography solvent (small enough that the line was not drowned by the solvent) which is made of 1 part acetone and 9 parts petroleum ether. The lid was placed on the jar and was only removed once the pigments had separated and the solvent had almost reached the top.

Read More

Macromolecules Lab Report

1283 Words | 6 Pages

Potato The mixture began to turn greenish at the bottom of the tube. At the end of the experiment, the mixture and become completely

Read More

Effect Of Temperature On Amylase

1167 Words | 5 Pages

5 water bath were set up each to10 °C. (5 were used do the experiment faster) 5 cm3 of starch solution were added into the 5 test tubes that were labeled test tubes. Then 5 cm3 of amylase enzyme was added into the other 5 test tubes that were labeled. Put one of the starch solution test tube (preferably the one labeled 1) and one of the test tube containing amylase into the water bath (10 °C).

Read More

Effect Of Enzyme Concentration On Enzyme Reaction

1046 Words | 5 Pages

The 3 concentrations of enzymes were 0.5 ml, 1.0 ml, and 2.0 ml of turnip extract, while the substrate consisted of 0.1ml, 0.2 ml, and 0.4 ml of hydrogen peroxide. In a separate tube, the control was made up of turnip extract and guaiacol, known as the color reagent. This was recorded the absorbance every 20 seconds for 3 minutes.

Read More

Aim Of Enzyme Catalase Lab Report

1973 Words | 8 Pages

The enzyme found in different conditions which its specific reaction rate. Variation in enzyme concentration, variation in pH, variation in temperature, and the effect of different concentrations of inhibitors were all tested. The enzyme concentration increased the reaction rate. An optimum pH and temperature were found for the enzyme, outside of this optimum the reaction rate would be lower. The correct name is the catalase enzyme.

Read More

Optimum Temperature Lab Report

1238 Words | 5 Pages

There was a big difference between result shows that the result obtained was not precise and reliable. One of the chicken liver could be smaller causing lesser catalase to be released whereas another chicken liver could be larger therefore more catalase was released. When there was more enzyme available , more hydrogen peroxide could bind to the active sites of catalase leading to the higher rate of reaction calculated. One way to improve on this method is to weight the chicken liver after cutting it with a smaller knife. By weighing the chicken liver after cutting into 1 cm3 , the mass of chicken liver can be measured and avoid having different sizes of chicken liver which will cause the imprecise result in the experiment.

Read More

More about Lab Report Organic Compound Catecholase

Open Document