The purpose of this project is to further test the effect of pH levels, and how they affect the production of enzymes. The hypothesis for this experiment was the more basic the ph buffer the high the activity of the enzyme. The more acidic the pH buffer the less activity of the enzyme. The first time the experiment was done with only three pH levels that were tested. That helped find the range of pH levels that were needed to test the second time. The data from the first experiment concluded that pH level of 4 and pH level 7 had the most activity. Thanks to the chemistry lab the pH levels were created and data was collected. In both experiments, there were procedures that were done differently that could have had an effect on the data. The