Enzyme Catalyzed Reaction Lab Report

The purpose of this experiment was to determine what effects temperature, substrate concentration, and pH levels would have on a catalyzed reaction. What I hoped to find was that temperature to cold or to hot would denature the enzyme. Another is if the substrate concentration and pH level is too high or too low it may enable the enzyme. All three factors will affect the enzyme catalyzed reaction in some way or form. With previous knowledge that enzymes are proteins , and proteins work best under specific conditions I expect by changing the three factors the catalyzed reaction be affected . If the enzyme becomes denatured the substrate will not be able to bind to the active cite, and if the enzyme becomes too cold the reaction will never occur

Each substance has almost a similar effect on the catalyzed reaction but differ in some ways as well. Enzyme catalyzed reaction are important for mostly all living organisms because these reactions are occurring within cells, and these reactions help to speed up the reaction rate and change peroxide into oxygen and water so the cells are not destroyed. The first experiment the effects of enzyme concentration on the reaction rate was calculated by placing three different amounts of concentration into the solutions and monitoring the reaction rate using a spectrophotometer. The three amounts of concentration included; 0.5ml, 1.0ml, and 2.0ml of enzyme. The amount of enzyme concentration did have an effect on the catalase. When the level of concentration was increased the movement of the enzymes also increased. As the enzymes are moving more rapidly they begin to bump into one another or interact with one another. Eventually the enzymes will reach a peak because there is a limited amount of substrate available that can bind to the active cite and this can cause the reaction rate to change. Viewing the graph below it can be