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Enzyme Lab Report

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ANSWER: When the amount of enzyme is increased the reaction rate is increased. The liver contains the enzyme catalase that eliminates the hydrogen peroxide to break it down the oxygen and water. 2. Is there a “control” (control group or control sample) in this experiment? What is it? Review the concept of a “control” in experimental design if necessary. ANSWER: Yes there is a controlled group and it is in test tube A. 3. What information does this control provide? ANSWER: When there is no enzyme present, there is no reaction, 4. Why do we add water to some of the test tubes in this experiment? ANSWER: Water helps with enzyme solidity and helps promote high performance for a reaction rate. PART A – QUESTIONS: 5. Create …show more content…

3. (a) What effect does an extreme high temperature have on enzyme function? (b) What is the cause of this effect, in other words what exactly happens to the enzyme molecules due to extreme high temperature? Explain your answer in detail and supported by research. ANSWER: In the higher temperature the enzymes in the liver were being broken down from the meat being cooked and making the enzymes nonreactive. 4. What happens to the liver homogenate when you cool it back down to room temperature and add more hydrogen peroxide? ANSWER: Once the enzymes were nonreactive and broken down, they did not respond to the hydrogen peroxide. PART B – QUESTIONS: 5. Create a bar graph describing your observations of the effect of temperature on catalase activity. All parts of a graph should be properly labeled or identified, including a short descriptive title for it Label the “x” and “y” axes with a brief description and identifying their measurement units properly. Your graph must be completely clear for anyone. You may lose points if anything in your graph is incomplete or unclear. Insert your graph in the space

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Enzymes are a form of protein that lowers activation energy and speeds up reactions as a catalyst. They are made by the stringing together of an abundant amount of amino acids and folded into a specific shape for chemical reactions. Turnip Peroxidase is the enzyme used in this lab and is derived from the vegetable. Enzymes are not used up or permanently altered by their environment Peroxidases are found in a range of organisms and function to break down alcohol (H2O2) and creates byproducts of oxygen and water. In this experiment, the reducing agent guaiacol is added with the substrate, hydrogen peroxide, to create water and oxygen.

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The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.

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By using a spectrophotometer to measure absorbance at 420 nm, the rate of enzyme activity after all reactions have come to a stop can be

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7. Will the temperature effect the pH Scale? Hypothesis: The hypothesis of this experiment is that the rate of reaction will increases well as the hydrogen peroxide concentration. If the temperature, pH and enzyme concentration is kept constant then the rate of reaction will start to decrease as well as the hydrogen peroxide concentration. Aim: To investigate the effects of changing the concentration of the enzyme catalase that it has on the rate of breaking down the Hydrogen Peroxide solution.

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These include, but are not limited to different pH levels, changes in temperature, amount of inhibitor, and amount of salt. In the reaction done in the experiment, the enzyme is known as peroxidase and the substrate is known as hydrogen peroxide (H2O2). In every reaction, there are constants such as the buffer

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molecules produced per unit time). In order to do so, enzymes bind temporarily to one or more reactants, lowering the amount of activation energy needed and thus speed up the reaction. Most of the cells use an enzyme called catalase to reduce the amount of hydrogen peroxide, a toxic substance that could accumulate as a result of normal cell activity. The purpose of this experiment was to observe the effects that enzyme concentration and copper (II) sulphate would have on the rate of reaction. This experiment was divided into two parts: Part A and Part B.

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After record your data and determine the absolute rate of the enzyme-catalyzed reaction. Based on the data and observations the hypothesis was accepted. It was accepted because when pH were changed to a variety of levels the transmittance began to get higher reaction rates. The increased absorbance means greater amount of product and a higher reaction rate will be produced.

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purpose the propose of this experiment was too see if the chemical reaction of a enzyme can be made faster. Hypothesis I think that a warm environment would be best to make an enzyme’s reaction faster. because a protein can move faster in heat.

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The instructor began our experiment by creating a catecholase enzyme solution using a chilled potato. He did this by removing the skin of the potato, this was necessary because the catecholase is contained under the skin. Another, reason is because if the skin is left on then the reading of the spectrophotometer would be inaccurate since the skin is brown as well as the benzoquinone. The potato was kept chilled so that the enzymes were not denatured. Next, the instructor chopped the potato and placed the pieces in a chilled blender along with 500 milliliters of chilled, distilled water.

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Conclusion In conclusion, the reason why this lab was conducted to examine the effects of Hydrogen Peroxide coming into contact with a catalase solution, in this experiment it was Calf Liver. As a result of the contact, oxygen gas was formed. Therefore, the collected data suggested the rate of reaction of the catalyse increases as the volume of Hydrogen Peroxide increases. This can be seen through the linear trend line that moves up in a positive direction, which shows that the relationship between the rate of reaction and the volume of hydrogen peroxide is positive.

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Claim This lab was conducted to test the effect of adding hydrochloric acid (HCl) to the reaction between hydrogen peroxide (H2O2) and the enzyme catalase, which is and enzyme found in liver (NOAA Office of Response and Restoration). Our group first hypothesized that if 2 milliliters (mL) of hydrochloric acid was added to a mixture of 2 mL of liver extract and 2 mL of hydrogen peroxide, then the acid would intensify the reaction between the hydrogen peroxide and the catalase and cause increased bubbling. Hydrochloric acid is strong and highly acidic (Merriam-Webster), leading our group to believe that it would create more bubbling. However, since the evidence collected did not support this research, adding hydrochloric acid to the liver extract

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Catalase and Temperature Introduction Background: Enzymes are catalysts which help reactions inside of organisms such as cells. Many different types of enzymes are used to catalyze different types of reactions. Enzymes are able to catalyze reactions that normally wouldn’t be possible under the specific circumstances in the cell such as the pressure or temperature of the cell. The way an enzyme works is it binds with the active site of a substrate and creates an enzyme substrate complex. The enzyme then breaks apart the bonds in a substrate and then leaves unchanged after the reaction.

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Enzymes are proteins that acts as catalysts, increasing the rate of chemical reactions. With containing many different enzymes, enzymes prove to be viable molecules, as they can ultimately determine the many possible chemical reactions that take place within the cell.1With an enzyme able bind to the substrate through specificity, the substrate correctly orients itself binding to the active site. Once positioned, the enzyme can lower the activation energy of the reaction causing the chemical reaction to occur at a rapid pace. The efficiency of an enzyme can vary depending on the environment. Some factors may include temperature, pH, and differences in enzyme substrate concentration.

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is the results of the effect of enzyme concentration on the potato enzyme. The 100% enzyme concentration had the highest average rate of O2 production being 7.79ml/min. This is because when there is a 100% concentration there is more molecules of the catalase for the hydrogen peroxide to react with. As the concentration is being diluted, the concentration of the catalase is decreasing so the molecules are less likely to collide and react. When the enzyme is at a 0% concentration there should not be any 02 produced and this is where some errors may have occurred.

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Continuing, in the tests conducted, the higher the temperature the faster the reactions happened. Next, its time to move on to the societal benefits for this experiment. Investigating the effect of enzymes based on temperature could help us further understand body processes, because enzymes are a big part of body functions and reactions. Continuing my last statement, we could also improve our knowledge on how medicines affect the body and how we can make them more

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