Specimen 1 (Ladybug) & 2 (unknown) belong to Coleoptera
This is a general description of Coleoptera. Specific description of each specimen will follow.
Beetles form this order
Refer to picture (fiddler beetle) for general morphology. Mention head, thorax, abdomen, wings, elytra, and that beetles have an exoskeleton.
No other order has more species = largest order
About half of insect species are beetles (≈40%)
Everywhere except for polar regions (mostly in vegetation but some marine habitats)
Varied diet: from plant parts, to other insects, to fungus and dung.
Some members agricultural and industrial pests ex. Boll (sounds like “bowl”) weevil = cotton pest. Very destructive
On the other hand, some help control agricultural pests ex.
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Loaded wells and ran at 100 volts for 45 minutes.
Well 1 was loaded with the ladder which gave bands of 5000, 3000, 2000, 1500, 1000, 750, 500, 300, and 100 base pairs. This gives a standard to which the size of the other samples can be measured.
Well 2 was loaded with the positive control, which contained Wolbachia 16S rRNA gene and insect CO1 gene. If one or both of the specimens were positive for Wolbachia, it could be measured against this control.
Well 3 was loaded with the negative control which only contained insect CO1 gene. This was to ensure our samples did in fact have insect DNA and wasvsomewhat of a check to make sure we collected the right specimen and ran the procedure correctly.
Well 4 was the no template control (NTC) and contained everything (master mix, loading buffer, etc.) that the other samples contained, except for template DNA. To ensure that there was no contamination.
Well 5 contained specimen 1, which was positive for insect DNA but negative for Wolbachia DNA.
Well 6 contained specimen 2, which was positive for insect DNA but negative for Wolbachia DNA.
Our specimens were in fact insects as indicated by the Gel results, however, neither were positive for