INTRODUCTION
Kaffir lime oil is essential oil from kaffir lime leaves (Citrus hystrix. DC). That essential oil possesses some important bioactivities such as antioxidant, antileukemic, antitussive, antihemorrhage, antioxidative stress properties, and antibacterial properties. Nowdays, essential oil of kaffir lime is in demand as a fragrance in the food, perfumery and cosmetic industries. Citrus hystric leaf is rich in vitamin E. Among 62 edible tropical analysed for α-tocopherol content citrus hystrix leaves (398.3 mg/kg edible portion) ranked second behind sauropus androgynus (426.8 mg/kg edible portion) [1]. Thirty-eight constituents were identified in the leaf essential oil of kaffir lime representing 89% of the total oil [2].
The oil was
…show more content…
Yellow FeCl3 5% solution is added to the nanoparticle solution with 5% ratio of FeCl3 solution: nanoparticle solution (3 drops: 1 ml). The mixture is then stirred slowly for 30 minutes. The colored nanoparticles solution is then observed with the light microscope (Advanced, Indonesia) using OPTILAB application.
Total essential oil Total essential oils analysis method is conducted based on Ali [7] with modification. 0,1 gr of kaffir lime oil nanocapsule is weighed and diluted to 100 ml using aquadest, taken as much as 1 ml (100x dilution) to put in the reaction tube then 1ml of saturated NaCO3 solution is added to the test tube and incubated for 10 min at room temperature. Then 0.5 ml of the folinciocalteu (Chemix CV, Yogyakarta) reagent and 7.5 ml aquadest were added, the mixture homogenized using vortex and then incubated for 30 min at room temperature under dark environmental conditions. Absorbance of the sample was then measured using a UV-vis spectrophotometer at a wavelength of 770 nm. The total phenol content of the sample was interpreted to be equivalent to gallic acid based on the standard curve of obtained gallic
…show more content…
The kaffir lime nanocapsule powder is shown on a two-sided adhesive placed on an aluminum cradle. Then the sample is coated using gold and then observed under SEM with 1000x magnification.
Particle Size Distribution The particle size distribution of kaffir lime oils nanocapsules was analyzed using the Horiba SZ - 100 Nanopartica tool. The nanocapsules and nanoparticles were dispersed into aquades by dilution of 10 × and then inserted into the tube and measured into the Horiba SZ-100 tool.
RESSULT AND DISCUSSION
Essential Oil Staining
One method that can be used to prove the compounds in kaffir lime oils is successfully encapsulated is by essential oil staining with ferric chloride (FeCl3) compounds. Ferric chloride will react with several organic compounds and produce a certain color according to the type essential compounds in the organic compound