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Lab Experiment: Osmosis Visking Tube Lab

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Lab Report Title: – Osmosis Visking tube lab Research Question: Does increasing the level of sucrose increase the procedure of osmosis? Introduction: This experiment is called the osmosis visking tube. This experiment is to investigate the relationship between solute concentration and the movement of water through semipermeable membrane by the process of osmosis. The purpose of this The Visking tubing apparatus establishes the osmosis procedure. The Visking tubing is a semipermeable membrane filled up with concentrated sucrose solution. The surface of the semipermeable membrane symbolizes the visking tubes and the mixture demonstrates the cytoplasm. If the Visking tube is absorbed in water, after a period of time, it will be have water inside water, this is because the water molecules can pass through the tubing, while the larger sugar molecules cannot diffuse out from the tubing because the size of sugar molecules do not allow it to go through the tubing. Therefore, this experimentation indicated that the sucrose molecules cannot diffuse from the concentration of the dilute solution, while on the other hand the water molecules diffuse from the dilute solution to the concentrated one. Hypothesis: If the (independent variable) sucrose of the dilute solution is …show more content…

It justifies the idea that increasing the concentration of sucrose does in fact speed up the rate of osmosis, an therefore increase the mass of the Visking tube. This happens due to the water molecules moving from a high water concentration to a low water concentration. Another reason why this hypothesis is correct is because sucrose particles are too large to go through the membrane therefore the sucrose does not diffuse into the water. According to the mean graph there is a clear outline that indicates constant acceleration, which justifies that the increase of the independent variable affects the dependent

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Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable pH 3. Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity 1.

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Additionally, it was difficult obtaining a piece of rhubarb that was thin and particularly red, therefore the effect could not be best observed in the cells. Part B: Design your own experiment Parts of this practical were taken and slightly altered from the following link http://www.markedbyteachers.com/gcse/science/investigate-the-effect-of-surface-area-on-osmosis-in-potato-tissue.html Aim: To observe the effect different surface area: volume ratios have on osmosis in potato tissue. Hypothesis: If the potato has a larger surface area: volume ratio, the quicker osmosis will take place and the larger the mass will be at the end of the experiment, therefore the difference in mass of the potatoes from the start of the experiment to the end of the experiment will be larger. Additionally, the potato pieces left in a saltwater solution will decrease in mass, whereas the pieces left in water will increase in mass.

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55 degrees celcius Table 6: Effect of Sucrose Concentration on Sucrase Activity Optical Density 35 g/L 30 g/L 25 g/L 20 g/L 15 g/L 10 g/L 5 g/L 0 g/L 1 1.007 0.974 0.950 0.926 0.849 0.734 0.515 0.003 2 1.002 1.011 0.947 0.937 0.834 0.766 0.496 0.002 3 0.980 0.998 0.944 0.932 0.838 0.754 0.495 0.001 average 0.996 0.994 0.947 0.932 0.840 0.751 0.502 0.002 Effect of Sucrose Concentration on Sucrase Activity 5. State how sucrase activity changes with increasing sucrose concentration. First sucrase activity increases greatly. After 10 g/l sucrase activity continues to increase but at a slow rate until it reaches 30 g/l. At 30 g/l to 35 g/l sucrase activities mostly stayed the same

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This was calculated using the C1V1=C2V2 formula. A photo spectrometer was used to measure, in arbitrary units, the change in membrane permeability of the B. Vulgaris cells. To begin, the B. Vulgaris samples were put into vials containing the distilled water, 40% and 70% Ethanol

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This also shows how much water was transferred from the egg to the sugar solution. The higher the percentage of corn syrup was, the faster the rate of osmosis was. As a result, the egg placed in 75% corn syrup, 25% filtered water had the lightest weight (0.5 oz), while the egg placed in 25% corn syrup, 75% filtered water weighed 2 oz. Overall, the importance of this lab was to understand the process of osmosis and how it differs depending on the type of

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In this lab we used two processes called Diffusion and Osmosis. Diffusion is the movement of molecules from areas of high concentration to areas of low concentration. Diffusion is a process that requires no energy and involves smaller non-polar molecules. In Figure 1 you can see the molecules spreading throughout the glass from the area of high concentration, so that the areas with low concentration are filled evenly as well. The other process was osmosis.

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More specifically the aim was to investigate what effect 40% and 70% ethanol solutions had on a B. Vulgaris cell membrane and then compare them to the same test with distilled water. It was hypothesised that the ethanol solution would increase the membrane permeability. From the results the hypothesis can be supported. The topic of cell membranes have been extensively researched, meaning that there is no limit to information and sources of information of the subject. The effects of alcohols on membrane have also been researched quite extensively.

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For this lab I will be using water and sucrose to demonstrate the rate of osmosis. In this lab I will be exploring how temperature impacts the rate of osmosis by placing pieces of potato of equal size in solutions of different temperatures and observing the change in mass of potato after a given period of time. The change in mass will indicate the rate of osmosis.

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Osmosis Lab Report Research Question: How does the change in the concentration of a sucrose solution affect the process of osmosis in a potato cell by measuring its mass? Background information: 1 Osmosis is the process by which a liquid passes through a semi-permeable membrane, moving from an area with a high concentration of water to a low concentration of water. There are various factors that affect osmosis such as: concentration, surface area and temperature. The concentration of solutions can affect the rate of osmosis, as there is more difference in the concentration of the solutions, which means osmosis, will take place quicker. Surface area could affect osmosis based on the ease by which molecules can get through the semi-permeable

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Sodium Chloride, is soluble in water, because it is polar and has an alternating negative anion and positive cation. Water is also polar, so like will dissolve in like. Therefore, the negative part of the chloride ions are attracted to the positive side of the water molecule and the negative side of the water molecules is attracted to the positive sodium atom. The negative oxygen charges in water, attract to the positive sodium ions in sodium chloride. Sucrose is soluble in water, because it is a polar molecule and the polar molecules in water attract the positive and negative regions around the sucrose molecules, which allows sucrose to be soluble in water.

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Biology Design Practical Joshua Edwards What are effects of the volume of a potato and the amount of weight it loses when placed in salt solution? Introduction This design practical uses a potato’s surface area to volume ratio to see what affects it has on osmosis in different concentrations. Osmosis is the movement of water molecules through a cell membrane into an area of a higher solute concentration. The movement goes the way of the solvent with more solute because the lower solute concentration is drifting through balancing the ratio of solute per solvent (En.wikipedia.org, 2018).

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The experiment shall use several concentrations of sucrose solution and a substance known as Methylene blue. A piece of potato/ carrot shall be placed in a boiling tube and the solution shall be poured into it. This tube shall have Methylene blue added into it. After incubation some of this solution shall be taken out with a pipette and inserted into a separate boiling tube containing the same sucrose solution however this solution shall be known as the pre-incubated solution. The drop shall be watched so as to see if the density of the water and concentration of sucrose has increased or not, displaying the water

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