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Mystery Microbiology Test

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Introduction: In the field of microbiology being able to identify a specific bacterial species is an important skill. In order to discover and being able to identify any microbial bacteria, there a list of test one must perform in order to come with the right microorganism. It is fundamental to be aware of the risk of toxify, the resistance to antibiotics and determining how to prevent its growth and being able to destroy this bacteria. By being able to run both physical and chemical test to determine the identity of the mystery microbe is a unique and useful skill in the field of medicine and microbiology. Each …show more content…

After collecting the information, then the top candidates will be eliminating using identification table, and the Lab manual and consulting the results obtained. Comparing and contrasting and go further with results would be essential to predict what the mystery microbe could be. Also further use of the manual plus web resources to make final selection of the Mystery microbe. This decision will be defended with an argument supported with the necessary data from the result table, plus the flow chart. The ultimate objective of this report is to narrow down the possibilities of bacteria using a series of test, while eliminating unlikely choices. Using the skills in the microbiology lab you can discover and make a educated decision of determining the mystery microbe, with the enough supported information collected from the chemical test and stains. There will be 17 test that will be run, each of this test will bring an important clue in determining the Mystery Microbe based on their environmental …show more content…

Nitrate reduction in the gram negative bacteria was conducted and when bacterium produce nitrate reductase when grown in a medium containing nitrate, the enzymes will convert the nitrate to nitrite. If nitrite is present the medium will turn red. This indicates a positive test (Goldman). If the bacterial is one of the few specials that can produce the enzyme Urase, this will be the key test that will separate this gram negative from all of the other possible candidates. Narrowing down the unknown microorganism to gram negative, this approach was helpful to take the next step, in some bacteria the cell wall is surrounded by cell enveloped called capsule, also some bacteria make capsule when faced in a harsh environment to protect them. A capsule stain was preform, the results were analyzed and observed. An additional procedure that was done, was the Fast Actin staining which helps to see if the bacteria contains Mycolic acid in their cell walls, which determines the structure and function of the cytoskeleton in living and fixed cells (Shah). As expected for both E.coli and K. Pnenumia the fast acting results were negative. For both E.coli and K. Pnenumia the Oxidase test was positive a reaction was obtained. The EMB agar is also an important procedure test that helps distinguishing pathogenic microbes in a short period of time (Shah). In the result table it shows the

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Exercise 14: Unknown Identification Lab Report The purpose of the study was to identify the unknown bacterium using various biochemical tests in addition to using scientific methods in determining the outcome of the hypothesis. Each biochemical test will help determine the bacteria based on specific characteristics of each organism. I was giving unknown number 232. The first procedure that needed to be done after obtaining unknown bacterial mixture was to isolate the two bacteria in a pure culture using the streak plate method described in Microbiology Laboratory Manual Eight Edition. The material used was trypticase soy agar (TSA) plate, nutrient plate, starch agar, hydrogen peroxide, iodine reagent and microscope.

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These microorganisms are used to teach us how multicellular organisms came to be and how they can survive today. These small, microscopic organisms are so unique that the identification of them is paramount in the advancements of science. Knowing the chemical makeup, the shape, and the biochemical processes is important in identifying these organisms to understand how they survive and where. A number of tests can be ran on an unknown bacteria to determine their ideal

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Introduction: Microbiology is the study of the microbial world, which is composed of microorganisms that are too tiny to be seen with a naked eye. This project demonstrates the significance of correctly identifying unknown organisms with methods and techniques acquired in the laboratory as they can make or break one's ability to accurately isolate and identify various species. Studying microbes enables oneself to have an insight on not just their structure or how they function, but how they have an impact on organisms and their environment around them as they can be found nearly anywhere. The importance of the process of identifying unknown bacteria vary from determining the causative agent of a disease, to testing if a specific food or fluid is safe to consume, and simply knowing what is present

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After experiment on microscope under oil immersion, I learned that my Unknown is gram positive. Under the lens, the bacteria appears in purple color. Its morphology is cocci arranged in cluster. However, during decolorizing process, I put too much alcohol to the crystal violet-iodine complex making the color overly removed. That led to the result of my gram positive has slightly redish

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