First, label one micro centrifuge tube +pGLO and another –pGLO. Using a sterile transfer pipet, transfer 250µl of competent cells (E. coli + CaCl2) into each tube and place them in crushed ice. Examine the pGLO plasmid DNA solution with the UV light and note your observations. Pipet 10µl of pGLO plasmid into the +pGLO tube and mix, close and return it to the ice rack. Do NOT add plasmid DNA to the –pGLO tube. Incubate the tubes on ice for 30min and make sure to push the tubes all the way in. During this time, label 4 agar plates with: +pGLO LB/amp, +pGLO LB/amp/ara, -pGLO LB/amp, and -pGLO LB, the nutrients and ampicillin should be integrated within the agar. Next, one of the most important steps, heat shock, is used to assimilate the plasmid