Pill bugs (Rollie Pollie): The Effect of Isopod Behavior on Wet vs Dry Stimuli
Abstract:
There are two main purposes for this experiment. First, isopods were observed so that their behavior could be recorded. Second, we exposed the isopods to a wet cotton ball and dry cotton ball, to determine which stimuli the pill bugs (or Rollie pollies) preferred over the other. In the experiment, five different pill bugs were removed from their usual habitat and placed in a choice chamber 1 pill bug at a time for a total 5 minutes each. It was observed that the pill bugs favored the wet cotton ball over the dry cotton ball. The pill bugs displayed positive behavior towards the wet cotton ball than dry cotton ball or undecided.
Theory:
Ethology is the
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The chamber has three connecting circles, two of the chamber are large and measures about three cm in diameter. In between the two larger connecting circles is a smaller connecting chamber that measures about three cm in diameter. The entire choice chamber is about 1.5 cm tall. To begin with this experiment you will pick up one of the cotton balls and wet it, then place it in the center of one of the large chambers. With the other cotton ball (the dry one), place it in the center of the other large chamber. Between each of the total 5 trials you are going to alternate the direction of the stimuli. With a soft bristled paint brush, you are going to now remove a rollie pollie of your choice from the petri dish. Place the rollie pollie in the central intersection of the smaller chamber, facing away from either cotton ball. Cover the chamber with a card stock box. Every 30 seconds for a total of 5 minutes, you are going to observe the isopods and record the location of where the rollie is at. Remember to cover the chamber back after observing the location, or the larger rollie pollie will climb out. When you have reached the total of 5 minutes on the first trial, remove the rollie pollie and take it back to the original habitat your instructor had them in. you will repeat the steps over and over until the last pill bug has been observed on trial