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Red Potato Osmosis Experiment

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Introduction Purpose The purpose of this lab is to study soil PH, soil salt concentration, osmosis and light. To identity potato weight change in sodium chloride. To determine the growth of plants scientist, farmer and business owners often work to determine the optimal conditions for their plants, which involves a variety of test and a large amount of trial and error over many years. I made a predication that the growing condition for red potato in this case is hypotonic. When the potato was placed in the 0.0m NaCl solution water diffused into the potato making it swell. The optimal NaCl condition …show more content…

These factors include, temperature and pH. Lower temperature like freezing lower enzyme activity. That is why food is preserved in a fridge for a longer period. Cold temperature does not denature enzymes, cold temperatures do not provide the activation energy needed for reaction to occur, even when an enzyme is present. Higher temperature makes enzymes to denature and so changes its shape and makes it perform a different function. Extremely high temperature like boiling (100 degrees Celsius) decrease enzyme activity. Most enzymes work best at body temperature of 37 (degrees Celsius). PH is another factor that affect the rate of enzymes activity. Enzymes function slowly in lower pH like in the pH of 1 or 2. Potato are high catalase when is being placed in a pH of 2 solution and hydrogen peroxide is added to it there will be some fizzing but not a good one. If the potato is placed in a pH of 13 and hydrogen peroxide is added there wouldn?t be any fizzing or little fizzing meaning that high pH either denature the catalase or substrate can not bind to the active site of an enzymes because its changes its shape. A potato piece in pH7 will work very well in hydrogen …show more content…

I measured and marked 3cm from the bottom of a small test tube. I filled the tube to 3cm marked with our assigned pigment solution. I then turn on our spectrophotometer and I loaded it with a test tube filled with distilled water into the slot in the machine and closed the lid. I then pushed the test button I pushed again the stored tests option and I pushed load test (chlorophyll). I pushed run test and then I pushed tabular. I pushed collect baseline. Took blank out and set in rack. I loaded my pigment solution tube and closed the lid. I pushed measured sample. Then the machine run for some period and gave me a list of

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Because of the fact that reactions are catalyzed by enzymes when they randomly collide with substrate molecules, increasing the temperature would increase the reaction rate. Increasing the temperature further increases the vibrational energy of the enzyme molecules, straining the bonds that keep them together. Furthermore, when the temperature is higher, more bonds will break because of these strains, causing the active site of the enzymes to change too. Similar to pH, a change in the shape of the active site leads to the substrate not being able to fit perfectly, leading to the enzyme not being able to catalyze the reaction. Overall, an increase in temperature will cause the rate of reaction to increase initially due to the increased kinetic energy.

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The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.

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Additionally, it was difficult obtaining a piece of rhubarb that was thin and particularly red, therefore the effect could not be best observed in the cells. Part B: Design your own experiment Parts of this practical were taken and slightly altered from the following link http://www.markedbyteachers.com/gcse/science/investigate-the-effect-of-surface-area-on-osmosis-in-potato-tissue.html Aim: To observe the effect different surface area: volume ratios have on osmosis in potato tissue. Hypothesis: If the potato has a larger surface area: volume ratio, the quicker osmosis will take place and the larger the mass will be at the end of the experiment, therefore the difference in mass of the potatoes from the start of the experiment to the end of the experiment will be larger. Additionally, the potato pieces left in a saltwater solution will decrease in mass, whereas the pieces left in water will increase in mass.

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Also, when equilibrium was achieved the water content of the potato decreased causing the mass of the potato to decrease by 1.4 grams from the start of the experiment. The last piece of evidence that shows diffusion occurred was that the salt had become more wet and soggy, this showed how the water in the potato’s cells had come out when finding the stage of

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Procedure and Observations To begin the lab, first all the correct equipment and materials had to be collected

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These factors include the pH and the temperature of the solution (1). Most enzymes have a preferred temperature and pH range (2). The preferred temperature for catalase falls between the ranges of thirty five to fifty degrees Celsius (4). Temperatures that are too high denature the enzyme and halt the enzyme’s activity (2). Catalase denatures starts to denature at fifty five degrees Celsius (2).

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For this lab I will be using water and sucrose to demonstrate the rate of osmosis. In this lab I will be exploring how temperature impacts the rate of osmosis by placing pieces of potato of equal size in solutions of different temperatures and observing the change in mass of potato after a given period of time. The change in mass will indicate the rate of osmosis.

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Catalase and Temperature Introduction Background: Enzymes are catalysts which help reactions inside of organisms such as cells. Many different types of enzymes are used to catalyze different types of reactions. Enzymes are able to catalyze reactions that normally wouldn’t be possible under the specific circumstances in the cell such as the pressure or temperature of the cell. The way an enzyme works is it binds with the active site of a substrate and creates an enzyme substrate complex. The enzyme then breaks apart the bonds in a substrate and then leaves unchanged after the reaction.

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By using the same mass of potato slices and putting them in different concentration of solutions for a specific amount of time will tell us how the concentration changes the mass of the potato slice. Therefore changing the rate of osmosis. Hypothesis: I predict that, if the piece of potato was put into a solution that has a high concretion of sucrose then the potato slice would lose mass as it would lose water from its cells because the water is moving out of the cell from a high concentration to a low concentration of water through a semi- permeable membrane. The cell is hypotonic and the solution is hypertonic.

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These enzymes have a secondary and tertiary structure and this could be affected by increases and decreases in temperature beyond the optimum temperature of the enzyme to work in. Mostly enzymes are highly affected any changes in temperature beyond the enzymes optimum. There are too

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Along with being found in plants, they are also present in liver cells, kidney cells, leukocytes and erythrocytes. For the concentration of enzyme experiment, the hypothesis was if the concentration of an enzyme increases, then the enzyme activity will increase as well. The hypothesis was proven to be true, because there are more enzymes to react with substrates. For the enzyme—factors affecting, the hypothesis concluded was if the temperature increases, than the enzyme activity will increase. This however was proven wrong, because enzymes become unstable at higher temperatures.

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The overall project goals and central questions that has to do with the project is mostly trying to determine the isotonic concentration of the salt in potato roots and the use of the ideal soil salt conditions for the potato plant growth. In part 2, we had to test the enzyme activity that is in the was involved in the potato, so we can also determine the ideal soil pH conditions for the potato plant growth. In part 3, we were able to test absorb the leaf pigment at various wavelengths that determine the optimum light absorption conditions and was able to make recommendations for the light conditions that would be used un greenhouses. The goal of the first project is to determine the ideal soil salt for potato roots and we can relate this to the project is to find out if Solution A or Solution B has more solute in it. The goal of this project is to determine the meaning of Osmosis.

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Biology Design Practical Joshua Edwards What are effects of the volume of a potato and the amount of weight it loses when placed in salt solution? Introduction This design practical uses a potato’s surface area to volume ratio to see what affects it has on osmosis in different concentrations. Osmosis is the movement of water molecules through a cell membrane into an area of a higher solute concentration. The movement goes the way of the solvent with more solute because the lower solute concentration is drifting through balancing the ratio of solute per solvent (En.wikipedia.org, 2018).

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The experiment shall use several concentrations of sucrose solution and a substance known as Methylene blue. A piece of potato/ carrot shall be placed in a boiling tube and the solution shall be poured into it. This tube shall have Methylene blue added into it. After incubation some of this solution shall be taken out with a pipette and inserted into a separate boiling tube containing the same sucrose solution however this solution shall be known as the pre-incubated solution. The drop shall be watched so as to see if the density of the water and concentration of sucrose has increased or not, displaying the water

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Enzymes are proteins that catalyze chemical reaction, and they work best at their optimal conditions (optimum pH, temperature etc.) but when the environment is not close to the optimum conditions, the enzymes denature and do not function anymore1. An excellent example would of the effect of temperature on yeast fermentation would be that the bacterial cells if exposed to very high temperature (above the optimal) would no longer function since their enzymes are denatured. The yeast would produce the most Carbon dioxide in the optimal temperature (45 °C ±1/°C) and other temperatures below the optimal temperature would not produce sufficient Carbon dioxide and any temperature above will produce too much that it will lead to the sinking of the bread and death of yeast because its enzymes have been denatured, therefore the reaction will stop. The bread will certainly sink if is not exposed to the right temperature the yeast will not ferment

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