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Pup Lab Report

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We hypothesized that men with apparently clinically localized CaP harboring occult metastases would also have elevated plasma levels of components of the uPA system of plasminogen activation that would be associated with a higher risk of biochemical progression despite effective local control of disease. Therefore, to determine the relationship of the major components of the uPA system with established markers of CaP presence, invasion, metastasis, and progression, we studied pre- and postoperative plasma levels of uPA and uPAR in patients with clinically localized CaP who underwent radical prostatectomy, patients with CaP metastases to regional lymph nodes, patients with newly diagnosed CaP metastases to bone, and healthy men. The availability of pre- and …show more content…

Postoperative plasma samples were collected between 6 and 8 weeks after surgery. Blood was collected into Vacutainer CPT tubes (Becton Dickinson, Franklin Lakes, NJ) containing sodium citrate anticoagulant and centrifuged at room temperature for 20 minutes at 1,500g. The top layer, corresponding to diluted plasma, was decanted using sterile transfer pipettes and immediately frozen and stored at 80°C in polypropylene cryopreservation vials (Nalge Nunc, Rochester, NY). For quantitative measurements of serum and plasma uPA, uPAR, and plasminogen activator inhibitor type 2 (PAI-2) levels,weused specific quantitative sandwich enzyme immunoassays (American Diagnostica, Greenwich, CT). Circulating levels of uPA and uPAR were higher in patients with CaP compared with healthy controls and decreased significantly after radical prostatectomy. In patients with CaP, plasma levels of uPA and uPAR were both higher in patients with CaP metastatic to bones than in patients with metastases to regional lymph nodes, who in turn had higher levels than patients with nonmetastatic CaP. Among

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Tn 4351 was originally isolated from bacteroides fragilis [30] . The transposon was successfully introduced into Cytophaga succinicans, Flavobacterium meningosepticum, Flexibacter canadiansis, Flexibacter strain SFI and Sporocytophaga myxococcoides by conjugation [25]. Tn 4351carries two antibiotic resistance gene. One of the codes for resistance to erythromycin and clindamycin which is expressed in bactroides but not in E.Coli. The other gene codes for resistance in tetracycline and is expressed in aerobically grpwn E. coli, but not in anaerobically grpwn E. coli or in bacteroides.

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1070 Words | 5 Pages

Over these 15 consecutive days our criminal justice class has watched the decomposition of 9 still born piglets, which were laid out in a specific way, with specific wounds. Piglet 10 was put into a box at the beginning of the experiment, and was not revealed until the end of it. Each piglet was in its own condition. Piglet 0 was naked in a box, Piglet 1 was naked on the grass, piglet 2 had 1st, 2nd, and 3rd degree burn all over its body, it too was laying on the grass, piglet 3 was stabbed behind right shoulder, also lying on the grass, piglet 4 had a .380 bullet would on its side, it was laying on the grass, piglet 5 was clothed on the grass, piglet 6 was naked piglet on the black top, piglet 7 was clothed on the black top, piglet 8 was suspended in the air with clothes on, and piglet 9 was suspended in the air naked. At the beginning of the experiment we hypothesized on theories we now know the answer too.

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341 Words | 2 Pages

On 05/06/17, at 11:43am, I Deputy Warden N. Christian was dispatched to 523 S Wayne Ave on a dog on dog or cat attack, no known owner. I arrived at the location and was met by Columbus Division of Police (CPD) and complainant Raquel Fuentes. I asked what happened; Ms. Fuentes stated that she was taken part in a community event by assisting with neighborhood clean-up with her dog (small white terrier) when a brown/white Pit Bull came out of nowhere and proceeded to attack her dog. With the assistance of passerby they were able to get the brown/white Pit Bull away from Ms. Fuentes dog.

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1054 Words | 5 Pages

Identification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.

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979 Words | 4 Pages

Shifa Sayeed can you check if this is all in past tense and if it has personal pronouns? The purpose of this experiment was to observe and thoroughly analyze how different substances of dissimilar intermolecular forces acted in different scenarios of evaporation, evaporative cooling, and boiling. In the lab, the three substances tested and compared were Acetone, Acetic Acid, and Propanol.

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559 Words | 3 Pages

Part A: Background on Gawande and his research In 1999, Atul Gawande published a paper on the “Cancer-Cluster Myth”, which he described at the beginning of the page as, “When a dozen people in a neighborhood develop tumors, it can’t be a coincidence. Or can it?” In other words, Gawande roughly defined the “Cancer-Cluster Myth” as a conglomeration of diseases concentrated in one area of living having to have some sort of connection. Gawande was a surgeon, writer, and public health researcher who practices general and endocrine surgery at Brigham and Women’s hospital (Atul Gawande, “Bio” 2014).

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534 Words | 3 Pages

Background and significance (1 paragraph, ~150 words) The NCI SEER program was created by the National Cancer Institute in the United States to collect and analyze cancer data from multiple sources to monitor trends in incidence, treatment, and survival rates. Researchers and healthcare professionals use the program's data to improve cancer prevention, diagnosis, and treatment. The information collected by the SEER Program encompasses various aspects of patient health, such as age, gender, and other demographics, as well as details about their cancer diagnosis, including the location and type of tumor, stage of the cancer at the time of diagnosis, and the initial course of treatment. Additionally, the program also tracks patient outcomes, including their survival rates based

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299 Words | 2 Pages

Three charts were missing DI follow-up data. The incidence of DI was 26% (47/180) in the early postoperative inpatient period (less than one week) and 9% (17/180) in long term follow-up. For Microadenomas, 21% (9/42) had DI in the early postop period and 9.5% (4/42) in long term follow-up. For Macroadenomas, 27% (38/138) had DI in the early postop period and 9.4% (13/138) in long term follow-up. Functional tumors and old age (>50 years) were associated with higher incidence of DI

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396 Words | 2 Pages

Moake et al. detected the presence of unusually large von Willebrand factor (ULVWF) multimers in plasma of patients with TTP and suspected that the ULVWF mulitmers were responsible for platelet clumping in the microvasculature. Moreover, they hypothesised that there was a lack of a depolymerase for the ULVWF (Moake et al. 1982). From then on, the lack of ULVWF depolymerase or the VWF-cleaving protease (VWFcp) was known to be responsible for TTP and was further established from then on (Lämmle et al. 2005; Furlan 2004; Tsai 2004; Moake

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1046 Words | 5 Pages

Clinical trials of Abraxane comparing to Taxol, showed a tolerable dose 50% larger than Taxol and improved tumor response in patients. Abraxane got approval for breast cancer and; later in 2012 and 2013; it was approved for Non-Small Cell Lung Cancer (NSCLC) and pancreatic carcinoma, respectively [82].

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2080 Words | 9 Pages

This paper therefore describes both conjugates, its pros and cons and recommends the best alternative. BACKGROUND Cancer, the leading causes of morbidity and mortality worldwide currently has chemotherapy, radiation therapy and surgery as treatment options [1, 2]. Radiation therapy and surgery are termed localized therapies because they are focused on getting rid of or stopping growth of tumors that are limited to localized areas [3]. Chemotherapy on the other hand plays a significant role in cancer treatment as it prevents not only local growth of tumor cell and tissues but also provides systemic treatment for metastases.

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382 Words | 2 Pages

During the talk of “understanding cancer through proteomics” by Danny Hill, I learned a different way of viewing cancer. Typically we look at genomics because it is easy to measure. This was something that biologists always seemed to get right. With detection and treatment of cancer you really must understand what is causing the problem. In genomics, you can only designate what may occur by the genetic code, however you cannot see the changes that are occurring daily and with treatments.

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1744 Words | 7 Pages

N0 indicates there is no lymph nodes contain cancer, N1 indicates that there are lymph nodes containing cancer. Metastasis is measured by M0 and M1. M0 indicates the tumor has not spread and M1 indicates the tumor is spreading. Pancreatic cancer is split up into four remaining categories. The stages 1-4 indicate the severity and type of tumor that is present within the body (National Pancreas Foundation, 2018).

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1005 Words | 5 Pages

According to the American Cancer Society, a malignant tumor is a group of cancer cells that can grow into (invade) surrounding tissues or spread (metastasize) to distant areas of the body (2015). The burden of

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1705 Words | 7 Pages

Degradation of fibrin is termed fibrinolysis. The fibrinolytic pathway is a complex physiological pathway controlled by action of a series of cofactors, inhibitors, receptors. Dysregulation of this pathway is associated with different pathologies (e.g. coagulopathies, disseminated intravascular coagulation (DIC) or congenital bleeding disorders). Degradation of fibrin is performed by serine protease plasmin, which is present in blood as a proenzyme, plasminogen, and needs to be activated by tissue plasminogen activator (tPA) and urokinase. Disturbance in haemostasis with abnormal bleeding may be caused by thromocytopenia, platelet function disorder, or defects in blood coagulation.

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