ipl-logo

Rp Uppc Case Study

1015 Words5 Pages

To optimize the RP-UPLC parameters and reach a good resolution and peak Tailing for Lamivudine,Abacavir and Dolutegravir, many chromatographic parameters were tested. Several mobile phases of different ratios were analysed to get that good resolution ,peak shape ,provided sufficient selectivity for the drugs. The phosphate buffer provided a higher sensitivity and selectivity than other buffers did. Using methanol and acetonitrile as organic components shown results in higher sensitivity, but varying the amounts of methanol and acetonitrile in the mobile phase affected the resolution , tailing factor theoretical plates and run time. Varying the pH of the mobile phase resulted in poor peak shapes, not showing good resolution. So we introduced potassium dihydrogen phosphate and di potassium hydrogen phosphate into the mobile phase to …show more content…

The limit of quantification (LOQ) and limit of detection (LOD) were determined based on the slope and the standard deviation of the response using the signal-to-noise ratio (S/N) as per ICH Guidelines Q2(R1) 2005. The LODs for Lamivudine, Abacavir and Dolutegravir were found to be 0.021, 0.330 and 0.038µg/mL respectively, and the LOQs for Lamivudine, Abacavir and Dolutegravir were 0.056, 1.320 and 0.095 µg/mL respectively. (table 6 ) Robustness Robustness of the method was performed by making slight deliberate changes in the analytical methodology like flow rate and solvent ratio. It was observed that this method did not significantly affect in system suitability parameters like USP tailing factor, theoretical plates and resolution, which confirmed that the developed HPLC method is

Show More
Related

Unknown Solid Acid

1800 Words | 8 Pages

Purpose: The purpose of this lab is to titrate an unknown solid acid (KH2PO4) with a standardized sodium hydroxide solution. After recording and plotting the data, the acid’s equivalence point will be recorded once the color changes. Using the equivalence point, the halfway point will be calculated, which is used to determine the acid’s equilibrium constant. The acid’s calculated equilibrium constant will be compared with the acid’s established pKa value.

Read More

Eastmamol/540 Week 4

563 Words | 3 Pages

This addition aids in controlling the reproducibility and retention. Separation of the mixture via RP-HPLC can be done using continuous gradient or stepwise to move out the sample components. For every separation, the ideal gradient and volume must be

Read More

OWCP Case Study

514 Words | 3 Pages

Amend provisions to require OWCP to work with USPS and other agencies to pursue Third Party collection on the behalf of their injured employees. In 2013, OWCP withdrew from the joint agreement for the USPS to pursue Third Party claims. Most interesting is that Third Party collection is an adjunct activity in relation to claim benefits. From the inception of the original joint agreement the between OWCP and USPS in November of 1980, USPS successfully negotiated third party settlements on behalf of USPS employees for 35 years. On March 10, 1985, the parties gave signature to a Memorandum of Agreement expanding the joint third party collect program for an indefinite period.

Read More

Nucleophilic Substitution Reactions Lab

1039 Words | 5 Pages

TLC was used to identify the actual unknown product as well as other products/reactants present in the filtered solution. The procedure was conducted by placing a TLC plate in a developing chamber that is filled with a small amount of solvent. The solvent cannot be too polar because it will cause spotted compounds on the TLC plate to rise up too fast, while a very non-polar solvent will not allow the spots to move. The polarity of the spots also determines how far it moves on the plate; non-polar spots are higher than polar ones. After spots on the TLC form, the Rf values are calculated and used to analyze the similarity of the compounds.

Read More

Dup15q Case Study

160 Words | 1 Pages

Short description: Although dup15q syndrome is a disorder that cannot be undone, many treatments are available to help address its symptoms. This page will explain why a multidisciplinary team can help treat dup15q and will also outline potential treatments for dup15q symptoms. Dup15q Treatment Why a multidisciplinary team? Because of the wide range of symptoms associated with dup15q, a multidisciplinary team of doctors can help determine what types of treatments to pursue.

Read More

Isp Case Study

484 Words | 2 Pages

On 4/18/20 SC completed a monitoring telephone call with Pa. SC asked Pa how is he doing and he reported that he is doing well. The Pa reported that his knees gave out as he was walking to walk up the steps, but no injuries were reported and the Pa did seek any medical treatment. The SC asked the Pa if anyone was present and if so who, and asked if the person (if any) helped him up. The Pa reported that his girlfriends were present and she helped up.

Read More

Pcp Case Study Lovlady

65 Words | 1 Pages

This is 24 year old white female who is here to establish PCP and complaining of sore throat, ear pain, and left armpit lymphnode enlargement. Patient is resident at Lovlady Center. She has history of IV drug use, no history of tobacco use. Patient reports past 3 days, intermitten fever, pain in her throat and right ear. She also reports enlarged lymphnode under her arm.

Read More

Bromohexane Lab Report

1111 Words | 5 Pages

It forms a complex with HBr and extracts it from the aqueous phase into the organic phase where the alkene is. This dehydrates the acid, making it more reactive so that the addition reaction is possible. Rapid stirring is required in order to maximize the surface area

Read More

Quetiapine Fumarate Case Study

3223 Words | 13 Pages

The mobile phase used was a mixture of ammonium acetate buffer and acetonitrile at a ratio of 400:600. A flow rate of 1 mL/min was maintained, and the detection wavelength was 292 nm (22). The required studies were carried out to estimate the precision and accuracy of the HPLC method and were found to be within limits [percent coefficient of variation was less than 15%]. Sample preparation briefly involved 0.4 μ membrane filter through which the sample was filtered, diluted with mobile phase, and 10 μL was spiked into

Read More

Plusnet Plc Essay

200 Words | 1 Pages

Plusnet plc is an English triple play network access supplier (ISP), which provides Broadband, landline and digital television. The organisation was established in 1997 in Sheffield, South Yorkshire, and turned into a public limited company in July 2004.On 30 January 2007, Plusnet was obtained by BT Group, however it keeps on working as a different and separate business. By December 2013, it had more than 750,000 clients over the UK. Plusnet likewise works various extra organizations: the Metro-net, Plusnet Hosting, Just The Name and John Lewis Broadband in association with John Lewis. This product is geographically segmented as it is a broad band provider that caters specifically for people who live in northern England, to provide them with

Read More

Realnetwork Case

606 Words | 3 Pages

RealNetwork creates digital media that people can use on a daily basis. RealNetwork products and services consist of RealMedia HD, which people use to stream movies and videos on their mobile devices of choice over the Internet; RealPlayer, which people use to watch videos, photos, and music on their PC; RealTimes, which organizes your photos to create a video story that is set to music and arranges them with different transitions; and GameHouse, which offers a host of games that can connect players on any devices they choose to use. RealNetwork has faced many challenges as they try to expand its brand. One of the challenges that RealNetwork faced is the elimination of their product through the Microsoft monopolization of the digital media market.

Read More

Organ Bath Experiment

1445 Words | 6 Pages

Next, a basic stock solution was used to prepare various concentrations ranging from 1.0 x 10-8M to 1.0 x 10-1M by serial dilution. The tissue was washed by overflow with reservoir’s solution for 5 seconds to obtain baseline before adding 0.1ml, 0.3ml and 0.5ml for each concentration respectively into the tissue bath. The tissue’s peak response for each final bath concentration(FBC) was measured and recorded. Rmax and EC50 of histamine were recorded.

Read More

Hammett Constant And The Hydrophobic (Hansch) Substituent Constant

1027 Words | 5 Pages

The substituents should therefore be hydrophobic and electron donating for maximum activity. • log 1/C is the concentration of drug that has a defined biological activity. The bigger the value of log 1/C , the smaller the value of c, the better the drug. • P is a measure of overall hydrophobicity. • π is the substituent hydrophobicity constant and measures the hydrophobicity of a specific region on the drug’s skeleton.

Read More

Rj45 Unit 5 Case Study

326 Words | 2 Pages

TD is responsible for providing at least one suitably sized network circuit to both data centers and terminating at a TDCT controlled firewall or router. This device will provide an Ethernet RJ45 handoff to RPM equipment. RPM will consider the RJ45 as the demarcation point. RPM suggests that this link be at least 100 mb’s and by year 4 it should be at an estimated 1 Gb/s or larger.

Read More

Buffer Lab Report

1344 Words | 6 Pages

The NaH2PO4 and Na2HPO4 powdered were weighted by using weighing machine, followed the mass that has been calculated in step (3). The NaH2PO4 and Na2HPO4 powdered were mix in a 500 mL beaker. 500 mL of distilled water were measured by using a 500 mL measuring cylinder, then is poured inside the 500 mL beaker containing both the powdered.

Read More

More about Rp Uppc Case Study

Related Topics

Open Document