Saccharomyces Crevisiae Lab Report

TITLE:

The Mating Of Different Strains of Saccharomyces Cerevisiae From Different Types of Fruits Samples

INTRODUCTION:

Saccharomyces cerevisiae is a yeast that are microorganism with a single cell. They have genetic content which is contained within a nucleus. This classified them as eucaryotic miccrorganism different with bacteria. Bacteria do not have nucleus and was classified as procaryotic organism. Yeast commonly found on flowers, soils, plants and also fruits. Yeast Saccharomyces cerevisiae is eucaryotic microorganism and also can be used in bioethanol production. Microbial ethanol is important today in increasing energy and fuel. In this practical, the production of yeasts for ethanol production from hexose and pentose sugars were

The empty weighing boat was placed into weighing balance and tarred it. 3.2 g of yeast extract, 4.0 g of peptone, 4.0 g of dextrose were weighted and added to the scott bottle that contain distilled water. The solution was mixed and dissolved. The solution was placed into autoclave and has been autoclaved at 121o C for 2 hours. After autoclaved, the solution has been cooled for 10 minutes. The solution was poured carefully into the sterile plates. The broth have been labelled and sealed with parafilm and stored in oven properly. The petri dish was keep in the chiller for the next

Firstly, mating of Saccharomyces cerevisiae using YEPD agar media on the plate. Five plates were divided by two and were labelled as MO1, OM2, OM3, OP1, OP2, OP3, PM1, PM2, PM3 and C (M for mango sample, P for papaya sample, O for orange sample and C for control). The colony of the subculture sample O was streaked on the plate OM1 and followed by colony of subculture sample M. Then, this technique was repeated with different colony on subculture sample O and M for OM2 and OM3. The similar tecnhique was used for OP1, OP2, OP3, PM1, PM2, PM3 and