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Sodium Chloride Reaction Lab Report

664 Words3 Pages

The purpose of this experiment was to observe and conclude the impact of a higher alkalinity and increasing sodium chloride concentration had on the yield of lysozyme crystals through crystallization. The constants for this process were sodium acetate at a concertation 0.05 M for every well in both rows, along with the concentration of polyethylene glycol (PEG) being set at 10% for every well in both rows. The two rows of wells that were tested deviated in alkalinity, row A having a pH of 4.5 and row B having a pH of 4.8, along with the sodium chloride concentration of each well. The concentration was increased by 0.5 M each well following the control well of 0.0 M sodium chloride. As seen in Figures 1-4, (blocks A1, A1*, and B1*,), no crystals formed when sodium chloride was not present. Figure 4 (block 1) you see what could possibly be some crystal formation, but this was determined to only be an obstruction, possibly dust, on the coverslip. Crystals were …show more content…

As seen in Figures 1 and 3 (blocks A2* and B2*) the higher yield of crystals formed were found in the higher alkalinity solution of row B. This confirms the first part of the hypothesis, that an increased alkalinity generates a greater yield of crystals. The second part of the hypothesis was concluded from looking at Figures 1 and 3 that as the salt concentration increased in each well, the yield of crystals increased. Combining both parts of the hypothesis into one and comparing the number of crystals formed in each row, row B with the greater alkalinity and with increasing sodium chloride concentration had the greatest yield of crystals. This greater yield can be contributed to the increase sodium chloride concentration and increased alkalinity, which disrupts the repulsion between lysozyme crystals during

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