Morpho-physiological and Biochemical Responces of Rubus idaeus under Salinity Stress In vitro Conditions Supplemented by Sodium Nitroprusside
Ali Ghadakchi asl , Ali- Akbar Mozafari*1, Nasser Ghaderi1
Abstract. To evaluate the effect of sodium nitroprusside (SNP) on morpho-physiological and biochemical characteristics of Rubus idaeus var. Danehdrosht, shoot tip explant was cultured on Murashige and Skoog (MS) medium supplemented with growth regulator combination including Benzyleadenin (BA)(1 mg.L-1), Indol-3-Butyric Acetic acid (IBA)(0.2 mg.L-1), Sodium Nitroprusside (SNP) (0, 50 and 100 µM) and NaCl (0, 50 and 100 mM).The results showed that salinity stress significantly decreased morpho-physiological and biochemical characteristics such as RWC, MSI, total soluble protein in regenerated explants. Furthermore salinity significantly increased the total
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idaeus sub cultured on MS basal medium was supplemented with0.8%(w/v) Agar and 3%(w/v) Sucrose and 1.0 mg.L-1 BA + 0.5 mg.L-1 IBA + NaCl (0, 50, 100 mM) with SNP at three concentration)0, 50 and 100µM(. The pH of the medium was adjusted to 5.8 and autoclaved at 121.5°C (model LS-100LJ, power 4.5 kw/AC220v 50HZ, Henan, China) for 15 min. SNP was used as the stock solution. SNP is very unsustainable to light and high temperature; the solution was prepared in the dark with distilled water and added to media after being autoclaved. When the temperature of the medium was reduced to 55-60°C, before gel formation in medium, it was sterilized with 0.2 µm filters. The culture jars were placed in growth chamber at 25±2°C under 16-h photoperiod (38 µmol m-2 s-1 PFD) using cool white fluorescent tube lights (Philips Ltd., Mumbai, India) and 60% humidity. Each treatment was included 6 replications and 4 explants per