ipl-logo

Spectrophotometry Lab Report

897 Words4 Pages

Spectrophotometry

Prepared for: Dr. Joseph Dasso
By: Lucy Onsarigo

Biology 1406 C5L
September 23rd, 2014

Introduction
Spectophotometry is the ability of molecules to absorb and transmit light energy for determining the concentration of substances in a solution. (Mark Garcia 2014). The instrument used is called spectrophotometer to distinguish different compounds since they absorb light at different wavelength. Some have wide range of wavelength and the shorter the wavelength the higher the energy. For one to know the absorbed light one has to put a cuvette into a sample holder with a solution and record the amount of light transmitted and absorbed through the solution. A concentration of protein is used, the reaction between …show more content…

We added 1 ml of distilled water to test tubes labelled 2, 3,4 and 5. . To tube 1 we added 1ml of standard protein solution and recorded the concentration of the standard protein as 10mg/ml. To tube 2 we added 1ml of standard protein solution and shake it so that it can mix well. With a fresh pipette we removed 1ml from tube number 2 and added it to tube 3 then gently shake the tube. With another fresh pipette we removed 1ml from tube 3 and added it to tube 4, shake well. With a fresh pipette we removed 1ml from tube 4 and added it to tube 5 and gently we shake the tube and another fresh pipette we removed 1mlfrom tube 5 and discarded this fluid. Tube number 1to 5 had the same amount of fluid in them but the concentration of protein was becoming weaker as we progressed to tube number 5 and this is what we used to develop our standard curve.
In the tube that we labelled “U” we added 1ml of unknown protein and into the tube labelled “B” we added 1ml of water. At this point we had six tube with water and protein solutions, the “U” with unknown protein and water but tube 1 to 5 with a standard protein solution and water. The tube “B” had only water in it so it was acting as our blank, a blank in our experiment was used to calibrate the spectrophotometer machine, it was identical to other tubes only that it did not have the solute that we were …show more content…

Our independent variable in this experiment was the change in concentration, so my hypothesis of the lower the concentration will have the lowest absorbance due to less molecules was right. We did the experiment correctly, since we wiped off our finger prints and set the machine to zero. In all the tubes apart from the one marked “B” we added protein which was the substance being determined, so “B” was our blank used to calibrate the machine. It contained everything apart from the solution that was absorbing

Show More
Related

Temperature Of Peroxidase Lab Report

1100 Words | 5 Pages

After the 15 minutes, each pair was removed from their assigned temperature and mixed with its partner. The mixed solution was then poured into the appropriate tube and placed in the spectrophotometer for 120 seconds. As peroxidase was broken down a brown color appears and is measured by the spectrophotometer. The absorbance readings were recorded every 20

Read More

Stoichiometry Lab Report

680 Words | 3 Pages

The cylinder was adjusted to the water level because that kept the pressure on the tube and the water inside at a constant rate throughout the tube. If the graduated cylinder had not been adjusted, the test would have been introduced to a random error because it would have been a flaw that was always present and would affect the measurements of the experiment. When the unknown anti-acid was tested the first time, the results yielded a 40.2% yield of CO2. The second trial of part B was not as accurate due to using a mass of the unknown-acid that was too small for accurate results. The lack of accuracy came from a lack of precision which resulted in a human error because the amount that was broken off and selected for the trial was too small.

Read More

Nt1310 Unit 9 Lab Report

1392 Words | 6 Pages

1. Identify the range of senses involved in communication • Sight (visual communication), Touch (tactile communication), Taste, Hearing (auditory communication), Smell (olfactory communication) 2. Identify the limited range of wavelengths and named parts of the electromagnetic spectrum detected by humans and compare this range with those of THREE other named vertebrates and TWO named invertebrates. Figure 1: the electromagnetic spectrum source: www.ces.fau.edu Vertebrates Human Japanese Dace Fish Rattlesnake Zebra Finch Part of electromagnetic spectrum detected ROYGBV (visible light) detected by light sensitive cells in the eye called rods and cones.

Read More

Allura Red Dye Lab Report

768 Words | 4 Pages

Using the value as a parameter, we then found the absorbance values for each dye at the concentrations of 0.0001, 0.00001, 0.00005, 0.00008 M, created a calibration curve, and solved for the extinction coefficients. Lastly, we put 3 drops of an 8.25% solution of bleach into the 0.001 M solution of allura red dye and 3 drops of an 0.825% solution of bleach into the 0.0001 M solution of sunset yellow dye

Read More

Tlc Lab

908 Words | 4 Pages

Five spots were labeled on the line and each amino acid standard was spotted on the plate using a capillary tube. The standards included leucine, alanine, phenylalanine, and lysine. The final spot was an unknown mixture of three amino acids. After allowing the spots to dry, the plate was placed in the developing jar and allowed to develop. The TLC setup is shown in Figure 2.

Read More

Catechol Oxidase Lab Report

642 Words | 3 Pages

Record the amount of absorbance by converting transmittance every 5 minutes for a total of 20 minutes. Repeat all of these steps for the cantaloupe, banana, replacing the blank each time to recalibrate the spectrophotometer. After recording all the percent transmittance value, the data was then converted into absorbance value by using the absorbance conversion table. The information was placed on a plotted graph

Read More

Alpha Galactosidase Lab Report

1488 Words | 6 Pages

Compare the result to the chart on the back of the urinary pH test strips bottle, and record data. Clean the stirring rod with water before moving on to the next test tube. Repeat this process for each increment (2 mL, 3mL, 4mL) Figure #1: Picture of bean solution mixed Figure #2: Picture of materials needed for the with alpha galactosidase experiment Safety considerations: Be careful with the beakers, glass stirring rod, and test tubes, as they could break easily and can cause cuts in the skin. DCP: A scatter plot will be used to display how the amount of alpha galactosidase (measured in mL) in the bean solution affects the glucose concentration (measured in mg/dL) and error bars to show the standard deviation.

Read More

Quantitative Spectroscope And Visible Light Lab Report

807 Words | 4 Pages

The wavelength (λ) and frequency (ν) of light are related through the equation: Using the following emission spectra: Calculate the frequency for the each of 8 emission lines: λ = c *v solve for frequency(v) v = λ/c a. Violet (450 nm) v = (3x108 m/s) /

Read More

Pglo Transformation Lab Report

680 Words | 3 Pages

Using two separate aseptic pipettes, 250 µl of LB broth were added to each micro test tube and mixed gently. Likewise, using two separate, aseptic pipettes for each tube, 100 µl of solution was added to the appropriate agar plate. After, using a new loop for each plate, the solution was spread gently across their surfaces. Lastly, the plates were stacked, taped together, and labelled before placing them upside down in an incubator set at 37°C

Read More

Unknown Compounds

1027 Words | 5 Pages

Each buffer was measured in a 100 mL graduated cylinder and contained in a 40 mL beaker. Once the reading of the buffer was stabilized, the program entered into reading 1. The probe was cleaned with distilled water and dried before being placed into the second buffer for reading 2. Once the second calibration was completed the pH probe was cleaned again. Next the probe was placed into the unknown solution.

Read More

Enzyme Lab

901 Words | 4 Pages

Light absorption occurs when atoms or molecules take up the energy of a light and reduces the transmission of light. The absorbance will increase with an increase in concentration while the transmittance will decrease with an increase in

Read More

Yellow Dye Synthesis Lab Report

701 Words | 3 Pages

The absorption peaks were then recorded, and compared to the theoretical values (the original green Powerade) by determining the percent error. # Overall, it was found that the yellow dye had a percent error of 6.76% and that the blue dye had a percent error of

Read More

Novembert Law Graph Of Absorbance Vs Protein Concentration

681 Words | 3 Pages

(Larsen, n.d.). A protein can be quantitatively assayed by many spectrophotometric methods. Generally, most methods used are by a chromophore. A chromophore is the color that appears when a molecule absorbs a specific wavelength of visible light and transmits or reflects others. (Encyclopædia Britannica,

Read More

Presence Of Macromolecules In Mcdonald's Happy Meals

1184 Words | 5 Pages

Starch solution is then placed into the test tube at a quantity of 5 mL. 5 drops of Lugol’s Iodine solution is added to the test tube. If the color changes, then it is known that starches are present in the solution. Proteins are next tested. In order to do this, 5 mL of gelatin solution is added to the test tube. 10 drops of Biuret’s reagent are added to test for protein.

Read More

Arginase Lab Report

1437 Words | 6 Pages

The absorbance level @ 520 nm obtained from the spectrometer indicates the amount of urea obtained via measuring the absorbance of the light through the supernatant coloration, which was provided by the

Read More

More about Spectrophotometry Lab Report

Related Topics

Open Document