Next, about 10 mL of both solutions, Red 40 and Blue 1, were added to a small beaker. The concentration of the stock solution were recorded, 52.1 ppm for Red 40 and 16.6 ppm for Blue 1. Then, using the volumetric pipette, 5 mL of each solution was transferred into a 10 mL volumetric flask, labelled either R1 or B1. Deionized water was added into the flask using a pipette until the solution level reached a line which indicated 10 mL. A cap for the flask was inserted and the flask was invented a few times to completely mix the solution. Then, the volumetric pipette was rinsed with fresh deionized water and
Glacial acetic acid and acetic anhydride were added to the mixture while refluxing, which converted the lime colored solution into a clear mixture. The flask was cooled in an ice bath and the solution
This proves that the egg has a higher solute concentration then the vinegar. However, when the egg was placed in the corn syrup, its masses greatly decreased. The corn syrup has no water molecules, so to create equilibrium, the egg transferred its water molecules to the corn syrup,
However, all proteins are constructed from the same set of 20 amino acids linked in unbranched polymers. The covalent bond that exists between amino acids is called peptide bond, hence a polymer of amino acids is named polypeptide. A protein is a biological functional molecule made up of one or more polypeptides which is folded and coiled into unique three-dimensional structure. In laboratory, it is important to measure the concentration of proteins for research investigations. Biuret test is adopted to quantify proteins in fluid by using a spectrophotometer.
Next, a 100 mL graduated cylinder was used to measure 60 mL of distilled water. The water was added to the compound and stirred with a glass-stirring rod until dissolved. Next, The flame test required the solution made during the solubility test. The experiment needed a metal wire that was dipped into the solution
This also shows how much water was transferred from the egg to the sugar solution. The higher the percentage of corn syrup was, the faster the rate of osmosis was. As a result, the egg placed in 75% corn syrup, 25% filtered water had the lightest weight (0.5 oz), while the egg placed in 25% corn syrup, 75% filtered water weighed 2 oz. Overall, the importance of this lab was to understand the process of osmosis and how it differs depending on the type of
The Study of Diffusion and Osmosis Using Deshelled Eggs Maquita A. Dieufene Jessica Thelwell(Partner) 10/09/2014 1611 Evening Lab Introduction It is quite simple to overlook the roles diffusion and osmosis play in daily life. If one has ever spent too much time in the pool and watched as their fingers begin to turn prune-like, that is an example of osmosis. Osmosis is simply defined as the movement of a concentrated solvent through a semi permeable membrane to a more concentrated solvent.(Biology Corner) Relating to the earlier example of osmosis, your body acts as the more concentrated solvent for the water to penetrate. Diffusion is the exact opposite of osmosis.
Introduction The intent of this experiment is to understand how hot and cold water interact with each other by combining clear hot water and black ice cold water. I hope to learn more about how hot and cold water interact with each other. As of now, I know that cold water is denser than hot water. Knowing this I formed my hypothesis.
The egg had been acting as the cell in this experiment. Placing the egg in vinegar made it easier to see the effects of osmosis because there was no impermeable shell to prevent liquids or molecules from passing through. As previously stated, water molecules will diffuse from higher concentrated areas of water to lower concentrated areas of water. In the tap water example, there had been a higher concentration of water molecules in the water, not the egg. This means that the water molecules will pass through the cell membrane to enter the egg and reach a state of equilibrium.
Bio Chem lab Report 04 Enzyme Biochemistry Group Member: Chan Man Jeun Duncan (16002621) Law Sze Man (16000478) Introduction Enzyme is a protein base structure substance in our body. It works at a biocatalyst that will catalyzing the chemical reaction, which helps to speed up the chemical reaction. Enzyme could only function in specific shape, and the shape of enzyme is depending on the environment, therefore it is hard for an enzyme to function well in an extreme environment. The aim of this experiment is to see can the enzyme functions normally in different environment(pH, temperature and salt concentration) via using starch solution, amylase from saliva, 0.5M HCl solution, 0.5M NaOH solution and NaCl solution, and using iodine solution
The mixture was then distilled. When the temperature was reached to about 59℃, half vial of distillate (1V) and 1 mL of the liquid residue (1L) were collected. For 61.0℃, the distillation was then continued. Samples (2V, 2L) were taken at about 61.0℃.
In order to utilize casein, bacteria cells secrete proteolytic exoenzymes (amylases, proteases, pectinases, lipases, xylanases and cellulases) outside of the cell that hydrolyze the protein to amino acids. The amino acids can then be used by cells after crossing the cell membrane via transport proteins [169]. Starch hydrolysis test is used to differentiate bacteria based on their ability to hydrolyze starch with the enzyme α-amylase or oligo-l, 6-glucosidase. These enzymes hydrolyze starch by breaking the glycosidic linkages between the sugar subunits. It aids in the differentiation of species from the genera Corynebacterium, Clostridium, Bacillus, Bacteroides, Fusobacterium and members of Enterococcus [170].
An egg naturally has a lot of stuff inside, so the outside solution has to be very concentrated for this to happen. That’s the case when an egg is treated with corn syrup or buried in salt. By contrast, when an egg is treated with distilled water, or a dilute salt solution, the solute concentration is higher inside the egg than out, so the water moves into the egg, increasing its mass. It may be easier to think about osmosis in terms of water concentration rather than solute concentration. If the solute concentration is high, then the water concentration will be low by comparison.
Enzymes are biological catalysts, which are essential for carrying metabolic reactions in the human body including the breakdown of food for digestion, absorption and energy production. All biological reactions within human cells depend on enzymes (Wolfenden 1). It is essential for humans to have well-functioning enzymes to break down large molecules into smaller units. As a matter of fact, in the absence of normal functioning enzymes, the human body would cease to exist because chemical reactions that are required to maintain the body function would not occur fast enough. I have a lot of interest in health and human nutrition.
Therefore, this experimentation indicated that the sucrose molecules cannot diffuse from the concentration of the dilute solution, while on the other hand the water molecules diffuse from the dilute solution to the concentrated one. Hypothesis: If the (independent variable) sucrose of the dilute solution is
