Bradford protein assay Essays

Finding out the protein concentration in three different drinks to see if the food labels are telling the truth. The Bradford Assay experiment will be conducted to find out whether the protein concentration is true on the food label. We will be collecting the absorbances of each of these drinks and making a standard curve chart that will show which drink is high in protein. The FDA is the Food and Drug Administration and they are responsible for protecting the public health and making sure that

“Nobody can give you freedom. Nobody can give you equality or justice or anything. If you're a man, you take it.”(a quote by Malcolm X) The play, ‘The Visit’, by Friedrich Durrenmatt backdrops a small town notorious town in Switzerland called Guellen. The visit raises the question of the corruptibility of justice by asking whether it can be bought in return for material wealth. The writer inquires whether a wrong judgement given years ages on the basis of false evidence, be used for justice years

ACIDITY TEST INTRODUCTION: Acidity is the total amount of hydrogen ion present in the food sample with the expectation of those bound to alkaline ions. The hydrogen ion can be either attached to acids or in the form of free ions or anions. Titratable acidity is different than total acidity although at times both terms are used to mean the same thing total acidity is the total amount of organic acids in the food sample. This all acids (tartaric, oxalic acid, citric acid, sulfuric acid, lactic acid

Chemistry Exploration Topic: determining the activation energy of a chemical reaction Research Question: What effect does temperature of the chemical reaction have on the activation energy ? ICT: Microsoft Word Autograph Microsoft Excel Introduction This experiment is designed to help in estimating the activation energy of the rate-limiting step in the acid catalyzed reaction of acetone with iodine. This is achieved by measuring the reaction rates at different reaction temperatures over

The purpose of this experiment is to learn about the principles of protein assays as well as to learn how to utilize the Beer-Lambert Law by doing various calculations such as how to calculate absorbencies, concentrations, and extinction coefficients. According to the Beer Lambert Law, absorbance is proportional to path length and concentration. For this experiment we will be learning how to use a spectrophotometer which measures transmitted light intensity. Spectrophotometers measure wavelength

amino acids of the protein, the dye shifts to the anionic blue form. As the protein and dye interact, an electron is donated to the charged groups within the protein so that the protein structure is disrupted and the hydrophobic pockets are exposed. The sulfonic groups of the dye bind to the amines within the exposed hydrophobic pockets to shift the dye to the anionic blue form. This color change is measured spectroscopically and is a direct correlation to the concentration of protein.10 Consequently

2. Methods 5 2.1 Size Exclusion Chromatography 5 2.2 SDS-PAGE 6 2.3 Bradford protein assay 7 2.4 Ion Exchange Chromatography 7 2.5 Soxhlet extraction method 8 2.6 Kjeldahl method 8 3. Materials 9 3.1 Size Exclusion Chromatography 9 3.2 SDS-PAGE 9 3.3 Bradford protein assay 9 3.4 Ion Exchange Chromatography 10 3.5 Soxhlet extraction method 10 3.6 Kjeldahl method 10 3.7 Enzymatic digestion 10 3.8 Used strains 11 3.9 General protein extraction method 11 4. Results 12

Materials and methods Gene cloning and protein over-expression in E.coli: The gene encoding Mbgl was amplified from the genome of Methylococcuscapsulatus(bath)with the following primers: forward primer- TGGTTGGTTCATATGAGCAGATACGAGTTTCCAGAGCGATTCCTCand reverse primer-TATATATTAAAGCTTATAGTCCCGATCCAGGACGGCACCGTTGGTC (restriction site NdeI and HindIII are in italics and underlined). Prime STAR HS DNA Polymerase premix (DSS Takara, New Delhi, India) and the set of above-mentioned primers were used in the

Being underestimated as just an athlete, has sparked the fire of someone who continues to make a difference in the world we live in. Tim Tebow is not only a well known professional athlete, but a tremendous role model in todays society. Tebow has many accomplishments on and off the football field. Charity and leadership are some of the many things that has led Tebow to make positive impacts in the communities all over the United states and other countries. Spreading faith and love to the people around

Sean Taylor is one of the greatest football players of all time. He was born on April 1, 1983 in Miami, Florida. He went to college at the University of Miami on an Athletic scholarship. In the NFL he played for the Washington Redskins. He died on November 27, 2007 after his house was broken into and he was shot. All over the NFL Sean Taylor is remembered. Sean Taylor is considered to be one of the greatest to ever play the game of football. Sean Taylor was born on April 1, 1983 in Miami, Florida

In the August 31, 2016 printing of The Oklahoman, an ad that is sure to catch most readers' eye is "The World's Biggest Hot Tub Sale," by Family Leisure. Most people who have ever sat in a hot tub would like to own one. Family Leisure is promoting this idea with this sale in bringing "Family and Fun Together." At this once in a lifetime sale buyers can save thousands of dollars. The purpose of the ad is to get people to own their own hot tub, target those that have stress and pain relief, and bring

The Colonization of the Americas in The Tempest In Shakespeare’s The Tempest, the characters are presented and described in a manner that makes them extremely similar to the natives and colonists that were involved in the English colonization of America. This is accomplished through the setting of the play, and by assigning the natives and colonists pseudonyms in The Tempest. In the play, Caliban represents the Native Americans while Prospero represents the colonists and their attempts to destroy

trypsin digestion of polypeptides Protein concentration was estimated by Bradford assay, and 100µg of total protein from each sample was subjected to in-solution trypsin digestion to generate peptides. Initially, treating the sample with 5µl of 100mM dithiothreitol in 50 mM ammonium bicarbonate for 30 min at 60ºC and alkylation with 200mM iodoacetamide in 50 mM ammonium bicarbonate at room temperature for 30 minutes reduced the protein disulphide bonds. Proteins were then digested with 4µg of sequencing

SDS-PAGE is one of the methods for determining the molecular weight of unknown proteins. SDS is an anionic molecule which denaturizes proteins and brings it back to its’ primary structure and it also provides a negative charge to the uncharged molecule. The SDS-PAGE enables the separation of proteins based on their sizes. The larger the size of the protein, the harder it is to travel through the gel thus heavier proteins stay near the cathode side of the gel. For this experiment, a software named Gel

2.5. Bovine Serum Albumin (BSA) binding Studies: BSA solution was prepared in 5 mM Tris-HCl/10 mM NaCl buffer solution to keep pH value constant (pH=7.4) and it is stored at 4oC, used within 2h. The BSA concentration was estimated spectrophotometrically (ε280=44,300 M-1cm-1) [35], 60 µM BSA was titrated with increasing concentrations of Ru (II) complex (0-50 µM). Absorbance changes at 280 nm were monitored by UV-spectrometry. 2.6. DNA Binding Studies: Binding studies of DNA were carried out at room

Catalase and Temperature Introduction Background: Enzymes are catalysts which help reactions inside of organisms such as cells. Many different types of enzymes are used to catalyze different types of reactions. Enzymes are able to catalyze reactions that normally wouldn’t be possible under the specific circumstances in the cell such as the pressure or temperature of the cell. The way an enzyme works is it binds with the active site of a substrate and creates an enzyme substrate complex. The enzyme

There are seven characteristics which make organisms different from non living things. These characteristics include growth, reproduction, irritability, movement, nutrition, excretion and respiration. The starfish is considered to be alive because it portrays the seven characteristics of a living organism. A starfish is considered to be undefined. It is continuously growing and all cells preserve the aptitude to develop into whatever section is considered necessary. several variety of starfish encompass

1.Introduction: An enzyme is a large protein that acts as a biological catalyst which changes the rate of a reaction. It provides an active site which is an environment where a reaction can take place this is made up of amino acids. The structure and shape of the substrate, the structure and shape of an enzyme and the substance upon which the enzyme works all have to match exactly. This enables the substrate to bind, but it can 't do this if the shapes of the two are different. The Aim of Enzyme

synthetic DNA, literally any DNA sequence may be created and introduced into any of a very wide range of living organisms.Proteins that can result from the expression of recombinant DNA within living cells are termed recombinant proteins. When recombinant DNA encoding a protein is introduced into a host organism,

What is the effect of surface area to volume ratio on the rate of diffusion of the colour from the agar jelly cube? INTRODUCTION: Diffusion is the movement of spreading particles from high concentration to low concentration in an environment such as a cell. This major procedure is used in cells to source them with nutrients, water, oxygen, and to transport unwanted wastes such as carbon dioxide out of the cell or to different cellular organelles. In this practical agar jelly cubes will be used