Coliform bacteria Essays

The presence of bacteria and pathogenic organisms is a concern when considering the safety of drinking water. Pathogenic organisms can cause intestinal infections, dysentery, hepatitis, typhoid fever, cholera, and other illnesses. Sources of Bacteria in Drinking Water: The Need for Water Testing Human and animal wastes are a primary source of bacteria in water. These sources of bacterial contamination include runoff from feedlots, pastures, dog runs, and other land areas where animal wastes are deposited

Introduction Bacteria can be found in bodies of water where it takes many different shapes and sizes. Bacteria can be found in clumps associated with a specific particulate matter. The enumeration of bacteria cannot be counted individual but by the amount of clumps it forms. When found in clumps, it is identified by a coliform in every colony. [4] Total coliforms are a type of bacteria formed from different types of sources. Fecal coliform is produced when the bacteria is fermented and incubated

human health and should be tested for fecal coliforms to maintain safety for the human population. Lake Byron, one of the water samples used in this experiment, will contain the most fecal coliforms because the surface of the water temperature is constantly rising to be ideal for growth of bacteria. The amount of positive Durham lactose broth tubes helped us to identify the MPN/100 mL of fecal coliforms. Inoculating an EMB plate with a positive coliform helped us suspect the possibility of E. Coli

I. TITLE: Fecal Coliform Testing ; M. Zinkievich , L. Moore ; APES ; Wends. Oct 21 2015 II. INTRODUCTION: Purpose: What is the amount of fecal coliform in my sample - from Fall Creek Reservoir? Is my water potable, safe for swimming and safe for incidental contact? Background: Fecal Coliform is a bacteria that grows in the intestinal tract, and is expelled during defecation. This means that where it is found has a source of raw sewage near it, being a leak in a septic system, or animal droppings

of the unknown bacteria. Gram Stain Gram stain is a common technique used to distinguish between gram positive and gram negative. Gram positive bacteria will be carrying a purple color of the crystal violet stains; whereas, gram-negative bacteria carry a hot pink color of the counterstain safranin. According to the result, the unknown bacteria are bacilli in a single arrangement. Their sizes are estimated at 2 micrometers wide. The

When given an unknown bacteria there are a multitude of steps one must go through to be able to correctly identify what bacteria was given. It is important to correctly identify the bacteria because some bacteria are more harmful than others. The gram stain is the first test that should be performed because it helps narrow down the possibilities by telling one whether the bacteria is gram positive or gram negative. After this test is performed, one shall place bacteria on/in Mannitol Salt agar, MacConkey

Introduction For clinical and environmental microbiologists, the rapid and accurate identification of unknown bacteria can sometimes mean the difference between life and death. Fortunately, microbiologists have a variety of different tools at their disposal to accomplish these tasks. In the field of microbiology, there are two primary methods employed in the identification of bacterial unknowns: biochemical testing and molecular analysis. While both methods are used frequently and to much success

appeared pink rods. Triple Sugar Iron Agar is a differential medium. It differentiates Gram-negative enteric bacteria based on the ability to reduce sulfur and ferment carbohydrates (lactose, sucrose, and glucose). This is indicated by the formation of acid and gas. Different strains of enteric bacteria utilize the nutrients differently hence can be differentiated. TSIA differentiates bacteria by their sucrose, lactose or glucose fermentation. Sodium thiosulfate provides the source of sulfur and the

During a lab session an unknown bacteria, Unknown #57, was assigned to determine its Genus species. A series of tests were performed with subcultures made when needed and antiseptic techniques used throughout. How bacteria differ from each other chemically as well as their shape can be determined by a differential stain known as a Gram stain. Gram positive bacteria have thick cell walls made of many layers of peptidoglycan which enable the bacteria to retain the crystal violet stain. In contrast

necessary to use a dilution series when isolating bacteria from a biological sample using spread plating? [5 MARKS] It is vital to use a dilution series to reduce the concentration of the original biological sample so it is easier to count the number of isolated colonies which are present on the spread plate. The diluted samples will have a workable number of colonies on the spread plate which can then be used to calculate the number of bacteria which were present in the original undiluted sample

Serratia: Serratia’s a group of gram (-negative), facultative anaerobic, rod-shaped bacteria of the Enterobacteriaceae family. Serratia are most common and pathogenic of the species within the genus, S. marcescens, is often the sole infective agent and frequently causes health facility infections. S. marcescens is often found in showers, bathroom bowls, and around wetted tiles. The bacterium is associate timeserving, human infective agent, capitalizing on its ability to make integrated surface communities

Contamination with pathogenic bacteria from the human/animal reservoir can occur when the landing place is unhygienic or when the fish are washed with contaminated water. Upon the processing of fish, its exposure to water is a critical part as pathogens may be present in the water used which can cause contamination and spoilage (Shoolongela & Schneider, 2012). Contamination of food can occur because of problems in food production, preparation, storage or cooking. The aquatic environment and soft

Hunting nightmare bacteria Answer the following questions Case of Addy (the girl from Arizona ) 1- Based on the pediatrician observations what was Addy’s diagnosis at the Pediatric Hospital intensive care unit ? She had got infected by staff or positive bacteria called Methicillin-resistant Staphylococcus aureus (MRSA). MRSA is a community associated bacteria that infects kids when they are playing in playing ground and getting scabs on their knee.They spread through that wound and

produces acid or not. MacConkey agar is an example of a differential media which will enable the organism to express a colour depending on whether it produces acid. This agar contains a pH indicator which can provide more information on the type of bacteria that is growing on the agar. Method A more detailed method is provided in the 280.201 Industrial Microbiology lab manual. However, the basic steps of carrying out this experiment are as follows: • Five agar plates are provided including: 1

The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that

Our experiment followed the genetic transformation of the bacteria E. Coli. Genetic transformation is the dynamic take-up of free DNA by bacterial cells and the heritable fuse of its hereditary data (Lorenz and Wackernagel 1994). In this case, the E. Coli was combined with green fluorescent protein to allow it to glow in the dark. Other components, such as arabinose sugar, uniquely composed pGLO, and the antibiotic ampicillin resistance, are all combined to create the fluorescence with a UV light

Hypothesis To achieve a negative results in food sample with the absence of Salmonella spp. 1.3 Introduction The genus Salmonella belong in the family of Enterobacteriaceae is a group of rod-shaped, Gram-negative, and facultative anaerobic bacteria, which grow with or without oxygen. It is also a non-spore forming and a motile or non-motile bacterium with exception of S.gallinarum and S.pullorum. The genus Salmonella consist of two species, S. enterica, and S. bongori. Salmonella enterica is

vegetative form or in form of spores. In fresh wound, bacteria have little time to multiply and there is practically no evidence of inflammatory tissue response, hence with few exceptions, bacteria are regarded as contaminants. But after a few hours, however, if sign of inflammation or other tissue response appear, then the bacteria must no longer be considered as contaminant but as infecting bacteria (Topley and Wilson, 1988).The commonest pyogenic bacteria often associated with infected wounds are Staphylococcus

Viruses are unique and completely different to all the other micro-organisms. They are not included in the Five Kingdom classification system. They are genetic entities classified as to exist between living and non-living states.They are the smallest type of microbe and sometimes described as an intracellular parasites. Viruses have simple structures that differ in size and shape. They do not have a nucleus, instead viruses have one or two strands of dna and rna to protect it. This coat is called

Following are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Beta glucouronidase test is used for the identification of Escherichia coli. An enzyme is produced by E.coli which is beta D glucouronidase. Beta d glucouronidase in turn hydrolyzes beta d glucopyranosid uronic derivatives to aglycons and D glucuronic acid. Bile solubility test is used in laboratory for differentiation of alpha hemolytic Streptococci