utilize the various RNA database websites provided to discover the other types of RNA and their characteristics, the features of tRNA and the secondary structures of RNA. Introduction Before the recent findings in RNA, RNA molecules were subdivided into three groups: Ribosomal RNA (rRNA), messenger RNA (mRNA) and transfer RNA (tRNA). These three groups are said to be the most common forms of RNA. They hold a prominent part of the functioning of a cell and the protein synthesis. RNA (or ribonucleic
DNA strand into messenger RNA for translation. There are three stages that take place in transcription; initiation, elongation, and termination. The first stage is initiation, which starts the whole process. During initiation the enzyme RNA polymerase binds to the promoter, which signals the starting point of transcription. When the RNA polymerase binds to the promoter the DNA strands unwind and transition from double-strands into single-strands. In the next step, elongation, the RNA polymerase adds
can be made into a protein. A ribosome is composed of one large and one small subunit that assemble around the mRNA. The mRNA now passes through the ribosome. Now, amino acid building blocks are carried into the ribosome attached to specific transfer RNA (tRNA) molecules. The small subunit of the ribosome arranges the mRNA so that it can be read it segments of 3 nucleotides. A group of 3 nucleotides is called codons. Each codon on the mRNA molecule matches a corresponding anti-codon on the base of a
making new DNA molecules from 4 nucleotide bases, using existing DNA as template. 7. new complementary nucleotide bases {A, C, G and T} to strand of DNA in 5 to 3 direction. This type of replication called continuous. Lagging strand: 5. Numerous RNA primer is made by primase enzyme and bind at various points along lagging strand. 6. Pieces of DNA called Okazaki fragment, is then added to lagging strand in 5 to 3 direction. 7. This sort of replication called discontinuous as Okazaki fragment will
DNA Transcription 1. a. The initiation complex contains messenger RNA, transfer RNA, and ribosomes. The process begins with the start codon AUG. The codons of mRNA pair with corresponding tRNA anti codons through hydrogen bonding. Transfer RNA carries amino acid specific to the anticodon, where every three nucleotides code for one tRNA. The ribosome structure includes two subunits. They join codons with corresponding anticodons, and amino acids. A continuous chain results because tRNA leaves behind
of DNA found in bacteria that controls gene regulation. Operons are controlled by an on switch known as the promoter. The Promoter is a place where the protein RNA polymerase binds. RNA polymerase is an enzyme that binds to DNA during transcription and unravels the DNA strands. RNA polymerase also transcribes the sequence of a messenger RNA (mRNA) molecule. (Goldberger, 1979, p.2) In this experiment the
elongation cycle peptide bond formation takes place between the amino acids bound to the ribosomal P and A site by their respective t- RNA molecules. During this step the amino group of amioacid at the ribosomal A site acts as a nucleophile and displaces the tRNA in the Psite resulting in peptide bond formation between the 2 aminoacids. This results in formation of dipeptidyl t-RNA at the ribosomal A site and a deacylated tRNA at the ribosomal P site. The reaction is catalyzed by enzyme peptidyl transferase
RNA = A C U G During transcriptions the mRNA is built up by commentary base pairing, using the DNA as a blueprint to construct the specific protein. CODON code for the bases to amino acids, once a molecule of mRNA has been transcribed it moves out of the nucleus via the nucleus pore. mRNA will be able to pass through the nuclear pore, which goes through the ribosome, production of protein through tRNA In the cytoplasm the mRNA combines with the ribosome cellular structure on which the polypeptide
sequence to the RNA sequence. As RNA production is the final outcome, it is also called DNA-dependant RNA synthesis. All types of RNA are transcribed from DNA, including: mRNA that codes for protein tRNA which is involved in translation rRNA which composes part of ribosomes snRNA which is involved in splicing and more less common ones Unlike DNA replication where the entire DNA strand is copied, only short sections of the DNA strand (including coding regions or genes) are transcribed to RNA. Also only
1a. Review: Describe three main differences between RNA and DNA. The three main differences between RNA and DNA are as follows: RNA has the sugar ribose instead of deoxyribose, which DNA has, RNA is single-stranded while DNA is double-stranded, and RNA uses uracil instead of thymine. 1b. Explain: List the three types of RNA, and explain what they do. The three types of RNA are mRNA, tRNA, and rRNA. mRNA carries the instructions to create proteins from DNA in the nucleus to ribosomes. rRNA forms a
What are genes? A gene is a piece of DNA that codes for RNA molecules. What is genetic engineering? Genetic engineering is the process where humans alternate the genome of organisms using biotechnological processes. It allows scientists to transfer genes from one organism to the next. FIGURE 1.1: A scientist manipulating an organisms DNA Genetics have been an impact on agriculture for thousands of years by the means of artificial selection. Farmers control the way their plants reproduce so that each
recyclable, workable copy of DNA, but in the form of RNA.” DNA has 4 nucleotides. (Nucleotide is linked to a phosphate group) Adenine, Guanine, Cytosine, Thymine. Adenine goes with thymine (A=T) and Cytosine goes with Guanine (C=G). The nucleotide bases are the genetic code (DNA and RNA molecules that carry information in the cells.) mRNA is made by an enzyme called RNA polymerase. It message will go down to the ribosomes for the making of proteins. The RNA nucleotides use the ribosomes instead of Deoxyribose
by the sequence of its nucleotide bases, which are composed of adenine, thiamine, guanine and cytosine. DNA undergoes transcription, which produces single-stranded mRNA, which uses uracil in place of thiamine. Next step is translation, in which the RNA becomes a protein, which then can act as structural units or enzymes. 2. How does DNA replicate itself? In order to replicate itself, DNA undergoes DNA replication, a process in which the DNA unwinds and splits in two. From that point on, new nucleotides
genomes. It is estimated that 2–3% of prokaryotic and 6–7% of eukaryotic genes code for DNA-binding proteins. Additionally, many of these proteins do not merely bind DNA, but also interact with other proteins and sometimes, as is shown in the example of RNA polymerase, only display theirfull activity when organized in multimeric complexes. SEQUENCE-SPECIFIC DNA BINDING Protein recognition of specific sequences on the DNA double
three main components of a nucleotide which are a pentose sugar which can be either deoxyribose or ribose, a phosphate molecule and one of the 4 nitrogenous bases e.g. Adenine, Cytosine etc. 1c) There are 2 bases which make two different bases in the RNA and DNA, which are Pyrimidines and Purines. Adenine and Guanine are purine bases because they are large bases and they each also have 2 rings of carbon and nitrogen atoms and purines also tend to be double ring bases. Thymine and Cytosine are pyrimidines
nucleus. The purpose of transcription is to synthesize pre-messenger RNA (mRNA) and make it into mature mRNA by using the information found in DNA. The more basic way of explaining it is to rewrite DNA into RNA. This process involves DNA, specifically promoter, coding and termination sequences. It also uses the enzyme, RNA polymerase, a protein complex that reads the DNA template and make RNA. The goal of transcription is to make messenger RNA or proteins. There are three stages of transcription: Stage
containing many RNA. The gene then unzips and unwinds from its zip because the Hydrogen bonds have been broken down between the nucleotide. The RNA that are free line up in one chain of DNA which is known as the template strand and they make hydrogen bond which is temporary with their complementary bases. Adenine from RNA pairs with Thymine from DNA, Guanine from RNA pairs with cytosine from DNA, Uracil from RNA pairs with adenine from DNA and this reaction is catalyzed from RNA polymerase enzyme
Each DNA molecule is made up of two strands which intertwine to form a double helix. The form of the stucture which looks like a ladder is each nucleotide base is a DNA strand which links with another nucleotide base via hydrogen bonds in a second strand of DNA. The bases cross link in a certain order such as A adenine will only link with T thymine and the other way around then C cytosine will only link with G guanine and the other way around. When reproduction is formed from parents to off spring
living organisms as the main constituent of chromosomes. It is the carrier of genetic information. RNA is ribonucleic acid, a nucleic acid present in all living cells. Its principal role is to act as a messenger carrying instructions from DNA for controlling the synthesis of proteins, although in some viruses RNA rather than DNA carries the genetic information. DNA contains the sugar deoxyribose, while RNA contains the sugar ribose. The only difference between ribose and deoxyribose is that ribose has
DNA stand for deoxyribonucleic acid. RNA stands for ribonucleic acid. They share some similarities, such as both being nucleic acids. Nucleic acids are long macromolecules that consist of a long chain of nucleotides. Both DNA and RNA have five carbon sugar and four nitrogen containing bases. In addition, DNA and RNA can be found in the nucleus of the cell. They are also vital to organisms. They are key to genetic information being created and distributed to various parts for the cell.