Introduction 1.1 Aim: To determine the kinetic parameters, Vmax and Km, of the alkaline phosphatase enzyme through the determination of the optimum pH and temperature. 1.2 Theory and Principles (General Background): Enzymes are highly specific protein catalysts that are utilised in chemical reactions in biological systems.1 Enzymes, being catalysts, decrease the activation energy required to convert substrates to products. They do this by attaching to the substrate to form an intermediate; the
Organic chemistry II Experiment 3: chromic acid oxidation reaction Introduction: Camphor is very compact and its molecular structure is symmetrical which gives it the property to change directly from a solid to vapour when heated. Therefore, camphor can easily be purified by sublimation technique. Sublimation is a phase change in which a solid phase passes directly into the vapour without going through an intermediate liquid phase. Solids which have vapour pressure below melting points can be purified
the cytoplasm. In this pathway, phosphates are transferred from glycolytic intermediates to ADP for the generation of ATP. The second pathway, the tricarboxylic acid (TCA) cycle, generates the reducing equivalents nicotinamide adenine dinucleotide (NADH) and flavin adenine dinucleotide (FADH2), which donate electrons to the electron transport chain to fuel oxidative phosphorylation (OXPHOS), the process by which ATP is generated in the mitochondria. Glycolysis and the TCA cycle can be integrated
vitamins B2 and B6, iron, and copper. Niacin is needed for energy metabolism of carbohydrates, proteins, and fats, and it is needed by the cells to enable the release energy. Nicotinamide, a derivative of niacin, is required by the body for making coenzymes NAD (nicotinamide adenine dinucleotide) and NADP (nicotinamide
Cellular respiration is a fundamental biological process that occurs in plants and animals and generates energy. Its chemical equation is 6O2+C6H12O66CO2+6H2O + ATP, the exact opposite of the second stage of photosynthesis (after the first stage during which sunlight is converted into Adenosine Triphosphate (ATP)). The circulatory system transports the glucose (C6H12O6) from the digestive system to the cells, the Oxygen (O2) from the alveoli to the cells, and Carbon Dioxide (CO2) from the cells to
Pellagra Luis Arguinzoni Florida Southwestern State College Abstract Pellagra is a nutritional disease that, if left untreated, can be fatal. It is caused by a deficiency in niacin, which is vitamin B3, and also the deficiency of the amino acid tryptophan. Pellagra has a variety of symptoms, but the main four categories of this disease are diarrhea, dermatitis, dementia, and eventually death. Pellagra is more prevalent in people with certain disorders, such as anorexia nervosa or alcoholism, or
ATP (adenosine triphosphate) is made up of a nitrogenous base called adenine and three phosphate groups that are both attached to a ribose molecule. The three phosphates are all negatively charged, causing a repulsion between all the molecules- making ATP a loaded spring. When hydrolyzed, ATP loses an inorganic phosphate to
The processing of NADH (nicotinamide adenine dinucleotide) is one important part of a mitochondrion’s function. Scientists used the tools available to them (high magnification) to observe the electrons being emitted from NADH. To take a look at these NADH molecules, the scientists used intravital
Cell respiration is a process in which our cells use to transfer the energy in our food which contains glucose, into ATP (Adenosine Triphosphate). ATP is a special molecule that cells use during cellular respiration to extract energy. The following chemical equation summarizes the chemical changes that occur in cell respiration to glucose when oxygen is available. C6H12O6 + 6O2 → 6CO2 + 6H2O + ATP There are 4 stages of cell respiration, the first major step is called glycolysis. Glycolysis
isotonic acids which can include both inactive oxygen species and radical species organic radicals. These radicals and species role is to attack multiple targets include: mycolic acid Synthesis, damage to DNA, lipid peroxidation and the nicotinamide adenine dinucleotide (NAD) metabolism in the cell. It is also responsible for the bactericidal activity of isoniazid. Isoniazid has inhibitory effect on mycolic acid synthesis and seems to be its main purpose. Evidence suggests that after treatment with isoniazid
light energy. The products of this stage are light, water, and carbon dioxide. The light energy splits water molecules into oxygen and hydrogen ions. Oxygen gas is released to the air as a by-product. Adenosine tri-phosphate (ATP) and nicotinamide adenine dinucleotide phosphate NADPH is released and used in the next stage, the Calvin cycle. The Calvin cycle is the process, in which trees turn carbon dioxide from air to sugar. This stage takes place inside the chloroplast as well but not in the granum
Introduction about pyridine ring. py ridine, any of a class of organic compounds of the aromatic heterocyclic series characterized by a six-membered ring structure composed of five carbon atoms and one nitrogen atom. The simplest member of the pyridine family is pyridine itself, a compound with molecular formula C5H5N It is structurally related tobenzene, with one methine group (=CH−) replaced by a nitrogen atom. The pyridine ring occurs in many important compounds, including azines and the vitamins
Two of the most important cellular energy cycles are aerobic cellular respiration and oxygenic photosynthesis. These two are critical in the continued sustainability of all life on earth as we know it. The two process several intricate stages in which the creation of cellular energy occurs, and have varied relationships with organelles located within the eukaryotic cell. Both processes are primordial and key in how life has evolved and become as diverse as we know it. Although aerobic cellular respiration
2H+ . Steps one to three is when the energy is invested. Steps four and five involve the glucose splitting into smaller molecules. Steps six to ten is when the energy is released in the form of ATP (Adenosine Triphosphate) and NADH (Nicotinamide Adenine Dinucleotide). In step one the enzyme Hexokinase uses the ATP to phosphorylate the glucose. This is an investment of energy. This is a crucuial part for the glucose as it is ready to be released for energy in a later stage. This step produces
Free radicals contain an electron (e–) as in case of superoxide (•O2–) radical and the hydroxyl radical (•OH) whereas reactive oxygen species (ROS) as a non-radical do not contains electron in hydrogen peroxide (H2O2) and organic hydroperoxides (ROOH). Radicals can react with other radicals or with non-radicals depending on availability of (e–). Initiation of free radical chain reaction occurs if polyunsaturated fatty acid is attacked by free radicals. This phenomenon occurs due to abundant molecular
chemical equation for the combustion of glucose in cellular respiration? C6H12O6+6O2→6CO2+6H2O+ATP / glucose + oxygen → carbon dioxide + water + energy How does ATP release stored energy? ATP (adenosine triphosphate) is a nucleotide that consists of an adenine base attached to a ribose sugar, which is attached to three phosphate groups. Energy is released when one of the three phosphate groups is removed by breaking a phosphoanhydride bond during hydrolysis. After this, ATP is converted into ADP (adenosine
Introduction: Obesity is one of the leading problems in western society today. Obesity describes people who are highly overweight and have excessive fat storage in their bodies. Obesity occurs when the daily intake of energy exceeds the daily output of energy and thus, causes the extra energy to be stored as fat which is built up over a period of time (Wilding J, 2001). Obesity is currently considered a disease by the World Health Organization (WHO) as these individuals runs the risk of developing
Chapter 5 Bacterial Growth Micro-organisms such as bacteria can be grown in the laboratory if essential components required for their survival are present. The cat of growing bacteria in the lab is referred to as culture and the environment on to which the bacteria is grown is called culture medium. The essential ingredients required for bacterial growth include: o Carbon source o Nitrogen o Metal irons o Presence or absence of Oxygen o Optimal temperature o Optima pH o Moisture Forms of Media
Aging is a significant issue in our world with billions being spent every year on anti aging products. The influence of media and culture in our society show aging as a negative and sometimes awful process. Unfortunately, this fearful and unfavorable attitude about the aging process has become more universal in our modern world. However, this has stimulated much research and a greater understanding of the factors that contribute to aging and what practices can potentially slow down the process. People's
b) Discuss the NADH, FADH2, production steps in the TCA cycle and explain the importance of the TCA cycle to function respiratory chain. The citric acid cycle refers to the first components that create during the cycle’s reactions- citrate / in it are protonated form citric acids. However series of reactions known as tricarboxylic acids (TCA) cycles, for three carboxyl groups on its primary 2 intermediates or the kreb cycles, after its discoverer Hans Krebs. Whatever citric cycles is a central driver