The tissue in the body is affected by the addition of Acetylcholine or Carbachol and contracts in response. In this experiment, the aim was to compare the response of six different concentrations of Acetylcholine and Carbachol on a section of rat ileum, in order to see which agonist had the greatest effect. To obtain results, the ileum was suspended in a tissue vessel and tied in place, while being submerged in Tyrode solution and each concentration was then added to the tissue vessel and the results recorded on a chart recorder. All concentrations produced a response from the ileum, therefore showing that the agonists had an effect on the tissues in the body. The graphs show that the equivalent concentrations for both agonists produced a similar