Enzyme Effect On Amylase

Enzymes are proteins that lower the activation energy needed for a chemical reaction in the body. The chemicals that they act on are called substrates. When combined with their substrates, the enzymes decrease the amount of energy needed for a chemical reaction to occur. Enzymes are similar to a lock and key, because one enzyme can only work on a specific substrate, just like only one key can fit into a lock. Each enzyme has an ideal environment, and if the environment of an enzyme changes, the enzyme’s ability could decrease. A factor that could decrease an enzyme’s ability could be a shift in pH level or temperature. In our experiment, we tested whether the change in pH or the change in temperature affect the function of amylase, which is

A Keto diet is a low carb diet. Because there are not enough carbohydrates to energize the body, the body will begin to break down fat as an energy source, which in turn causes weight loss. When you starve yourself, you are at risk for ketoacidosis, which causes high pH levels in the blood. We modeled this diet to see the effect it had on enzymes. To represent the blood of a person with ketoacidosis, we used a liquid that had a high pH level. Using the Lugol’s Iodine test, we investigated the effect of an environment with a high pH on the function of enzymes. In our experiment we added 15 drops of starch (amylose), 15 drops of an enzyme that breaks up starch (amylase), and 15 drops of the high pH liquid into a test tube. Next, we added 15 drops of the Lugol’s Iodine solution into the same test tube. We measured how long it took for the high pH test tube to change color versus how long our control took. The Lugol’s Iodine test identifies for complex carbs. In our case, if the substance changed to a light brown color, the test was negative and the substance contained like glucose, and if the substance changed to a dark brown or black color, then the test was positive and the substance contained complex carbohydrates like starch. The substance with the high pH changed to a light brown at a time of 12 minutes and 49 seconds and the control changed to the same color at an earlier time of 11 minutes and 15

It contains antioxidants that fight disease and slow the aging process. Alkaline has been said to cleanse the colon, and improve skin, muscles, and joints. According to it’s manufacturers, the dietary substance has also been linked to the advancement in one’s overall health. Using the Lugol’s Iodine test, we investigated the effect of an environment with a low pH, on the function of enzymes. In our experiment, with the help of a pipette, we put 15 drops of starch (amylose), 15 drops of an enzyme substance that specifically breaks up starch (amylase), and 15 drops of the Alkaline water, into a test tube. Next, we added 15 drops of the Lugol’s Iodine solution into the same test tube. We measured how long it took for the substances that we were testing to change from their dark brown color, but the substance with the Alkaline never noticeably changed color. The Lugol’s Iodine test searches substances for complex carbs. In our case, if the substance changed to a light brown color, the test was negative and the substance contained simple carbohydrates (like glucose), and if the substance changed to a dark brown or black color, then the test was positive and the substance contained complex carbohydrates (like starch). Since the substance with Alkaline water remained dark brown, and the control substance changed to a light shade of brown, this meant that the