Then five millilitres of sample “A” were placed in the test tube labeled “A”. This was then repeated with the next three samples. Each sample was visually observed and the colour of each was recorded. Next 20 drops of Benedict’s solution were added to each test tube and the test tubes were lowered into a hot bath at a temperature of approximately 80 degrees Celsius. All colour changes were recorded.
Catechol oxidase is found in cell cytoplasm, their function in plants are to "help protect damaged plants bacterial and fungal disease." The objective of this experiment is to test the presences of catechol oxidase in various fruits and vegetables. Our group hypothesis states that, If catechol oxidase is present in the selected extracts, the null hypothesis is that catechol oxidase is not present in the selected extracts. Next, the prediction would be, if catechol oxidase doesn't differ with other enzyme sources, then the rates will
The purpose of the Spinach Leaf Chromatography Lab was to determine how much chlorophyll a, b and carotenes and xanthophylls the Spinach leaf contained through measuring the distance traveled by the pigments. The hypothesis the group created was that a spinach leaf contained multiple pigments. The group after doing the chromatography of the spinach leaf then determined the rate or flow of migration using Rf(Distance pigment traveled/ distances solvent 2. Materials and Methods A 2x15 strip of chromatography paper was cut so that it would fit inside the test tube. Then a point was cut in the bottom 0.5 cm of the strip.
Test tube 1 consisted of an Elodea leaf placed in phenol red. A group member blew into the tube to add more carbon dioxide to the phenol red, which changed the color from red to yellow. On a scale of 1 to 10, with 1 equaling to definite yellow and 10 equaling definite red, we rated the initial color of the test tube a 2. The test tube was then placed in the light for 40 minutes. After the 40 minutes, the test tube color had converted back to red (rating =10).
A human heart cDNA library was screened with a probe corresponding to the mouse PPARg (7). Three overlapping clones were identified, purified, and sequenced. The nucleotide sequence is shown in Fig. 1. The longest open reading frame starting from the nucleotide at position 91 coded for a polypeptide of 505 amino acids.
ABSTRACT Due to the potential harmful effects of the glacial acetic acid, a commonly used diluting fluid for manual WBC count, the researchers proposed to use commercially-prepared ascorbic acid to lyse the red blood cells while maintaining the morphology of the white blood cells. This study aimed to compare the capability of commercially-prepared ascorbic acid as a lysing agent in a diluting fluid to glacial acetic acid. Specifically, this study aimed to compare their capabilities in lysing red blood cells, maintaining white blood cell morphology and white blood cell count.
The principal objective of this study was to understand how cell membranes are able to store pigments in their intracellular compartments which then prevents the pigments from entering the general cytoplasm using different concentrations of a solution which in this case was detergent (Huber, et al., 2018).Several slices of Beetroot were immersed in water and three different concentrations of Tween 20.It was then hypothesized that Higher concentrations of Tween 20 will increase the cell membrane permeability of Beetroot. The results were not consistent with the hypothesis and it was shown that with different concentrations of the detergent (Tween 20), the cell membrane permeability increased in the control and then followed the hypothesis pattern in the other concentration of the
Objective: The purpose of this experiment is to test various temperatures beet have on cell membrane and to investigate how beets will secrete red pigments. As the temperature increases in the cell membrane more dye will be release from the beet. As it expands, kinetic energy will accelerate up the distribution of red pigment to a point where it will damage the cell and the denature of proteins will increase where the dye will be free. Background:
Introduction: Cyanide is a known poison, inhibiting respiration. Inspite of its toxicity, it is a common industrial agent. The cyanide compounds in which cyanide can be obtained as CN- ions are classified as simple and complex cyanide. Discharge of cyanide into environmental water is a threat to living organisms. To regulate the discharge of cyanide, WHO has established standard of 50μg/L of HCN for drinking water.
Light intensity is one of factors affecting photosynthesis. Depending on distance of light intensity, the result would be changed. Prior to the experiment, the distance should be settled. Even though it was expressed by using tape, it would be inexact. Therefore, improvement to the method would be to make the higher light intensities so as to be quite accurate, which denotes lamps would be adjusted to a beaker containing elodea from side to side because the smaller light intensities would be less accurate since the light spreads out, and the elodea might be exerted influence on other experiments or background light.
The 3 concentrations of enzymes were 0.5 ml, 1.0 ml, and 2.0 ml of turnip extract, while the substrate consisted of 0.1ml, 0.2 ml, and 0.4 ml of hydrogen peroxide. In a separate tube, the control was made up of turnip extract and guaiacol, known as the color reagent. This was recorded the absorbance every 20 seconds for 3 minutes.
The purpose of the photosynthesis lab is to identify and observe the colors of pigments in a spinach leaf as they separate on chromatography paper. Photosynthesis is the anabolic process, or the process of making smaller particles into larger ones in metabolism, that plants use to convert reactants such as sunlight, water, and carbon dioxide into products of glucose and oxygen. Photosynthesis occurs in the chloroplasts of a plant cell where chlorophyll, a pigment, is used to allow the process to occur, while factors such as the amount of sunlight, water, and carbon dioxide affect how much photosynthesis occurs. Chromatography is the process of separating chemical mixtures by allowing chemicals to move over another substance (Woodford, 2017).The moving phase, or moving substance, is in one state of matter as it spreads across the stationary phase, or nonmoving substance, in another state of matter to be identified separately (Woodford, 2017). Chromatography works by adsorption, which means that as the molecules pass over the solid, the
In this experiment, antioxidants and vitamins were involved. Antioxidants are molecules which can safely interact with free radicals and terminate the chain reaction before vital molecules are damaged. Although there are several enzyme systems within the body that scavenge free radicals, the principle micronutrient (vitamin) antioxidants are vitamin E, beta-carotene, and vitamin C. Additionally, selenium, a trace metal that is required for proper function of one of the body 's antioxidant enzyme systems, is sometimes included in this category. The body cannot manufacture these micronutrients so they must be supplied in the diet. Free radicals are atoms or groups of atoms with an odd (unpaired) number of electrons and can be formed when oxygen
Jaspreet Singh Professor Paratore Biology 1 November 1, 2014 Spectrophotometry Identifying Solutes and Determining Their Concentration Statement of the Exercise or of the Problem The purpose of the lab experiment was to attain the following objectives: • Learning to Operate the Spectrophotometer • Construct absorption spectra for cobalt chloride and chlorophyll. Hypothesis If greater and higher concentrations of cobalt chloride are added to each solution then greater amounts of light would be absorbed by each solution. Thus a liner relationship will result in which the absorbance of a substance would be proportional to its concentration, which will be depicted, in a linear graph.
There are various membranes and all have a variation of functions. The tonoplast in beets, contains a water-soluble red pigment called betacyanin, this pigment is what gives the beetroots is distinctive purpleish red color. The betacyanin is soluble in water and insoluble in lipids. This means that the pigment is contained in the vacuole of the cell while it is healthy.
