Bacillus Photosynthesis Lab Report

In conclusion, a few of the hypotheses stated in the introduction were proven correct but most were not. In the beginning, it was thought that in three plates the bacteria could be separated, however, it actually took four plates to separate the bacteria. It was confirmed that both bacteria are gram positive. However, along with this one of my bacteria was not Staphylococcus. One being Bacillus was confirmed and actually both ended up being Bacillus species. It was thought that one bacteria would be catalase negative and the other catalase positive but they both were catalase positive. The hypothesis for oxidase was confirmed to be true and both were oxidase negative. Even though one was thought to be mannitol positive, they both were

One result that may have been misread is the citrate lab. According to Simmons, the results only take up to twenty-four hours, but then other websites state anywhere from forty-eight hours to five days to produce conclusive results (Simmons, 2012) & Aryal, S., May 08, 2017). It was suspected at one point that the one we thought was full separated to begin with may not have been as separated as we have thought. To try to keep this cross-contamination from occurring, areas that were suspected to be the other bacteria were avoided to try to ensure the other bacteria was being tested. There may have also been an error in the catalase test. A wire loop was used to conduct the test. After further research, it has been found that a metal loop or needle should not be used with hydrogen peroxide due to the fact that it can produce a false positive and degrade the metal. (Aryal, S., May 08, 2017). A final error that may have occurred and would explain the change in color of on unknown one is the fact that there is oxygen in the air which can react with dry