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Catalase Enzyme Lab Report

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1. What temperature will have the fastest reaction if a catalase enzyme and hydrogen peroxide are mixed together?
2. If catalase enzymes and hydrogen peroxide are mixed together, then the fastest reaction will occur when the test tube is placed in a 30 degrees C water bath because that is the temperature when and hydrogen peroxide react the fastest.
3. Amount of catalase enzyme added
b. 0.5 cm3
c. cm3
d. 10 cm3 test tubes. The catalase enzyme was added to each test tube, so the reaction was possible.
4. Height of Oxygen Bubbles
b. cm
c. 10 cm3 test tubes, 0.5 cm3 catalase enzyme, 4 cm3 of H2O2, and Certain Temperature (10 degrees C, 20 degrees C, 30 degrees C, 60 degrees C) The test tube is where the dependent variable that is measured. …show more content…

Exergonic Reaction with Catalyst

First, pour about 15 ml of substrate solution into a 25 ml beaker. Then, put the substance in an hot water bath and set it to 30‹C. Next, add a filter paper disk soaked in yeast solution and record the time it takes for the disk to rise to the top of the solution. Repeat steps 2 and 3 2 more times for 30 ‹C and 3 times for 40‹C. Finally place the solution in a ice bath for 20‹C and repeat steps 2 and 3 3 more times. First, pour about 15 ml of yeast solution into a 25 ml beaker. Then, add a filter paper disk soaked only in water in the substrate solution and wait until it sinks to the bottom. Next, add a filter paper disk soaked in substrate solution and it should immediately sink to the bottom. After, record the time it takes for the disk soaked in the substrate solution to rise to the top of the solution. Repeat the 3rd and 4th step 2 more times.
References:
• (2010, December 3). Structural Biochemistry/ Enzyme/ Gibbs free energy graph. Online Image. Retrieved October 22, 2015 from

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