Chloramphenicol Ophthalmic Hydrogel Lab Report

Methods

Formulation of chloramphenicol ophthalmic hydrogel

Formulations was prepared according in Table 1.

Poloxamer 188 and poloxamer 407 each weighed and dissolved with distilled water. Then stored in the refrigerator for 24 hours. Next chloramphenicol dissolved with propylenglicol, and nipagin. The mixture was stirred until the entire dissolved and homogeneous. The materials were ready each put in a bottle 100 mL size vial, then sterilized with autoclave for 15 minutes at 121 °C. The preparations hydrogel done within aseptic at LAF cabinet.

Evaluation of chloramphenicol hydrogel ophthalmic preparations

Organoleptic test

Organoleptic hydrogel checked by observing changes in color, odor and clarity. Clarity was checked visually by examination

Safety valve was released and the rotor was turned until a stable (± 2min) that was appointed by needle pointer. Measurement was taken during storage days 1, 3, 7, 14, 21 and 28.

Preparations of chloramphenicol eye drops

Eye drop was intended as comparison preparations to find out the effectiveness of chloramphenicol hydrogel. The Preparations in accordance with the formula in Table 2.

Antibacterial effectiveness test of chloramphenicol opthalmic hydrogel and eye drops against p. aeruginosa ATCC 9027 and s. pyogenes ATCC 19615

The purpose of the stage was to compare the effectiveness of antibacterial preparations in the form of hydrogel chloramphenicol against the form of eye drops. The testing was done by the method of diffusion in order. Testing conducted during storage days in 0, 1, 3, 5, 7, 14, 21, and

As many as 1 mL hydrogel preparations added in 1 mL of growth medium with stratified so that dilution dilution series made were 50%, 25%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%, 0.39%, 0.02%, and 0.01% by volume end of the tube was 1 mL.

Then as much as 1 mL of bacterial culture equal Mc. Farland 0.5 added into the test tubes so that the final volume of the tubes were 2 mL. All test tubes were incubated at 37 °C for 18 h. Turbidity test observed in the media and determined MIC value preparations.

Tube with negative results or does not indicate the presence of growth, then conducted subculture with solid growth media each bacteria as test assertion MIC value chloramphenicol preparations hydrogel. As many as 20 mL of growth medium was prepared and then given solids have zones for the variation of concentration with the method MIC macrodillution preparations showed the absence of growth. With the method of scratch, the results of the saucer MIC subculture incubated at 37 °C for 18 h. The results form bacterial colonies scratches subculture. If there were scratches (+) then showed the presence of growth, when no scratches (-) then the growth does not occur. The data obtained was made in the form of a