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Pglo Transformation Lab Report

449 Words2 Pages

To begin, during this lab experiment, genetic transformation was successfully carried out. After observing the agar plates, it was found that only the plate with ampicillin and no pGLO plasmid did not grow any of the E.coli bacteria. All three of the other plates grew the E.coli bacteria, however it grew differently in each plate. In the control plate where the pGLO plasmid, ampicillin, and arabinose were not present, the bacteria grew in the pattern that it was spread in originally. In the two other plates, bacteria grew in colonies that eventually joined together due to prolonged time in the incubator. Although transformation occurred in both plates with the pGLO plasmid, only the plate containing arabinose sugar had fluorescent colonies when observed under UV light. …show more content…

This explains why in the plate with ampicillin but no pGLO plasmid, the bacteria was unable to grow. If the pGLO plasmid did not contain ampicillin resistance, E.coli would not have been able to grow in the agar plates, rendering the experiment pointless. Secondly, the results exhibited the fact that arabinose allowed certain colonies in the E.coli bacteria to glow under UV light. Essentially, the arabinose acted as an inducer that binded to the repressor in the inducible operon, allowing the RNA polymerase to transcribe the DNA and synthesize the GFP, causing the bacteria to grow. In this case, the plasmid itself is not fluorescent because it is a gene and genes in DNA cannot glow themselves. However, DNA can code for certain proteins, such as GFP that would cause the bacteria to glow after going through genetic transformation, explaining why the presence of arabinose sugar caused fluorescence. For this reason, it makes sense that the transformed bacteria is fluorescent, but the plasmid itself is

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