ipl-logo

Human Genome Lab Report

844 Words4 Pages

Summary: The Sequence of the Human Genome
The Human Genome Project had been initiated by the National Institutes of Health and the U.S. Department of Energy with the goal of sequencing the entire human genome in 15 years. In order to sequence the human DNA, the DNA was obtained from five individuals, and plasmid clones were made using their DNA. The euchromatin of the human genome was sequenced using the whole-genome shotgun sequencing method. This method used mate pairs, which are paired-end sequences from subclone libraries. After using the whole-genome shotgun method, the sequenced DNA had to be assembled. Two assembly strategies were used- the whole-genome assembly and the regional chromosome assembly. The whole-genome assembly combines …show more content…

coli strain W1485, which was one of the materials used in this experiment. Many techniques were used such as DNA isolation, transformation, and restriction enzyme digestion. Restriction enzyme digestion by EcoRI gave a linear fragment on the electrophoresis gel because the plasmid had only one restriction enzyme sequence site. The generated size of the plasmid was the same as the predicted size.
The tetracycline-resistant gene of the R6-5 DNA may be separated from its site of replication by the EcoRI restriction site which can explain why tetracycline-resistant pSC101 plasmid was generated from R6-5 that underwent shearing, but no plasmids with tetracycline resistance were generated when R6-5 had been cleaved by EcoRI. Studies showed that R6-5 fragments that had been cut by EcoRI may be ligated in E.coli cells. These reassociated fragments can be functional plasmids if they are able to replicate, carry the antibiotic resistant gene, and able to form the circular shape of a plasmid.
The experiment was able to generate a mixture of plasmids pSC101 and pSC102. Because two plasmids were generated from R6-5 and were able to survive together in one host cell, it was assumed that R6-5 had at least two sites of

Show More
Related

Tn 4351 Unit 5 Lab Report

506 Words | 3 Pages

chinesis. A construct of R751::Tn4351 (the physical map of R751::Tn4351 and restriction sites are shown in fig. 7) was selected for introduction into F. chinesis to discover if the introduction and insertion of the vector R751 and the transposition of T4351 into the F. chinesis chromosome by a triparental mating occurred. One parent was E. coli GJ342 which carried a helper plasmid, the second parent was E. coli HB101 which contained R751::Tn4351 and the third parent was the F. chinesis target strain. 189 colonies were isolated on LB agar plates which in passage in fresh media were able to grow in 200µgml-1 erythromycin.

Read More

Pglo Lab Report

1426 Words | 6 Pages

Hypothesis: If the pGLO plasmid is inserted into E. Coli bacteria, then the surviving cells with the arabinose will glow green under UV light as they will be genotypically identical and phenotypically different. Experimental Design: In this experiment, recombinant DNA technology was used to modify the genetic properties of an organism. First a plasmid was created which consisted of ori, araC, GFP, bla and pBAD promoter. The GFP is a jellyfish gene that codes for the production of green protein; bla breaks down ampicillin which then can be used to select only the bacteria containing the bla gene by placing ampicillin in the growth medium.

Read More

Intercellular Gene Transfer Lab Report

1100 Words | 5 Pages

The Mut section is expected to have no growth as mutants require the amino acids leucine and valine to grow which is not provided in the minimal medium. Results Figure 2. Testing of mutant mixed with DNA, mutant bacteria and DNA on LB medium Growth was observed on the Transformed (Trsf) section and the Mutant (Mut) section but not on the DNA section. Due to human errors, the photo of our experiment was lost, but we have obtained similar results as from group1.1 and their photo is presented.

Read More

Pglo Lab Report

465 Words | 2 Pages

The purpose of this lab was to perform a procedure known as genetic transformation which allowed us to genetically engineer E. Coli to be ampicillin resistance. Before the lab we expected that lysogeny broth and minus DNA will have growth but no glow. The lysogeny broth, ampicillin, and

Read More

Unknown Lab Report

1706 Words | 7 Pages

Bacillus subtilis was used for positive control and Escherichia coli for negative control for endospore

Read More

Unit 7 Lab Report Sample

2270 Words | 10 Pages

Lab 7 Report: Molecular Methods - Group 5 Abstract. This lab report outlines the procedures and findings of an RT-qPCR experiment conducted on extracted DNA from E. coli K-12 samples. The objective was to assess microbial dynamics and investigate the efficacy of molecular methods in detecting specific pathogens. The experiment involved DNA extraction using silica columns and various buffers, followed by qPCR setup and analysis. Very precise procedures and protocols for biosafety measures and quality control, including technical replicates and dilutions, were used throughout the experiment.

Read More

Colistin Case Study

2242 Words | 9 Pages

Afterwards, with the use of Glimmer 3.02 and BLAST, gene prediction and annotation were completed with another plasmid, pHN122-1, as a reference. To confirm the role of the gene that caused polymyxin resistance and contained mcr-1, the gene and its sequence were place into a cloning vector pUC18 that yielded pUC18-mcr-1. With this yield and electroporation, it was used to transform an E.coli strain, revealing its ability to confer colistin resistance. Q3D:

Read More

Pglo Transformation Lab Report

914 Words | 4 Pages

On the second day after these colonies were grown, the lab begun by labeling two test tubes -DNA and +DNA, adding the transformation solution to to each, and placing it on ice. Then the procedure called for the use of a sterile loop to pick up a colony of E coli and add it to each test tube, then after E coli was in both test tubes, the pGLO DNA was placed in ONLY the +DNA test tube. After 10 minutes on ice, and after the 4 plates are labeled each to LB -DNA, LB/amp -DNA, LB/amp +DNA, and LB/amp/ara +DNA respectively, the test tubes are heat shocked to loosen the DNA cell membrane to allow pGLO to slip in and code within the DNA. After more time in the ice bath, the tubes are taken out and set at room temperature with the LB broth placed in both test tubes. Then the dishes LB/amp +DNA and LB/amp/ara +DNA were spread with the +DNA solution, and the dishes LB -DNA, and LB/amp -DNA were spread with the -DNA solution.

Read More

Lb Agar Lab Report

1005 Words | 5 Pages

The plate represented by Figure 2 should not have any bacteria growing on it as it contains the antibiotic ampicillin to which the E. coli bacteria are not resistant. The cells on this petri dish do not contain the pGLO plasmid etiher, which means that they would not produce the GFP (Green Fluorescent Protein) and thus would not fluoresce green when viewed under a UV-light. This results in a petri dish with no bacterial colonies. Figure 3 shows a Petri dish with 17 colonies on an LB nutrient agar plate containing ampicillin and the plasmid (+pGLO/LB/amp). On this plate there should be many colonies growing only if transformation was successful.

Read More

Summary Of Genome By Matt Ridely

980 Words | 4 Pages

From the title of this book, I realized that this book would be interesting to read. The author’s approach to explaining the human genome was indeed erratic and unprecedented. The author, Matt Ridely, thought about the human genome as a sort of an autobiography its own record of showing the history of our human species and ancestors from the very first start of life. Thus, he used all 23 different chromosomes and used one of the related genes to write themes of human nature. He emphasized the fact that knowing and learning about our human genome is very important as these knowledges would help us, human beings to revolutionize anthropology, psychology, medicine, paleontology and virtually every other science.

Read More

How Has The Human Genome Project Changed The Basic Structure Of Biology?

409 Words | 2 Pages

The study of the Human Genome Project has changed the basic structure of biology forever, as well as, the transfiguration of what we have come to know as medicine and technology. Moreover, what had started in 1990 and had been completed in 2003, The Human Genome Project, has allowed researchers to create a blueprint that maps out the entire structure of the human body, in order to pinpoint out the specific genes that cause issues in our fragile bodies. Due to this study, researchers are now able to treat, cure, or even prevent diseases and illness that researchers were unaware of prior to this study. In conducting this investigation, the hypothesis of The Human Genome Project was that of trying to figure out how the differences in the genetic

Read More

Personalised Genomics Research Paper

3123 Words | 13 Pages

BE257 Scientific Literature Darren Walsh GCB2 Personalised Genomics: Are we there yet? Signature:__________________ Date:______________ Personalised Genomics: Are we there yet? Darren Walsh GCB2 Introduction Personalised genomics (PG) is the application of genetic sequencing and gene analysis techniques to aid personalised healthcare. This aims to increase the efficacy and improve the timing of therapeutic and preventative measures against disease by availing of an individual’s genetic biological markers. Personalised genomics can be used in many different ways such as: prenatal diagnosis, disease prognosis, disease diagnosis, stratified risk assessment, genome informed treatment and research relating to lifestyle choices and

Read More

The Human Genome Project Is Important In World History

1357 Words | 6 Pages

The Human Genome Project is, “An international scientific research project with the goal of determining the sequence of chemical base pairs which make up human DNA, and of identifying and mapping all of the genes of the human genome from both a physical and a functional standpoint.” People say this project is still the world’s most collaborative biological project. This project will forever be important in world history for a lot of reasons. Like how it helped and still helps to solve medical mysteries, find a cure for many deceases and brought the world together to try to get the whole project done in a fast and timely matter. It also brought a lot more knowledge to scientists so they can discover more things because they have this

Read More

Human Genome Project Paper

948 Words | 4 Pages

The specific goals of the project were; “identify all the approximately 20,500 genes in human DNA, determine the sequences of the 3 billion chemical base pairs that make up human DNA, store this information in databases, improve tools for data analysis, transfer related technologies to the private sector, and address the ethical, legal, and social issues that may arise from this project” (U.S. Department of Energy Human Genome Project, 2015). The project officially started in 1990 and was completed two years ahead of schedule in 2003. All the goals of the project had either been met or exceeded expectations (U.S. National Library of Medicine, 2015). The Human Genome Project was a revolutionary project that exceeded all expectations and has provided present day medicine with new technology and

Read More

History Of The Human Genome Project

953 Words | 4 Pages

In 1984 the US Department of Energy, National Institutes of Health, and International groups held meetings to discuss studying the human genome. In 1990 the start of the Human Genome Project begins with the publication by the US Department of Energy and National Institute of Energy discussing the first five year plan and projecting it to be a 15 year project. Just five years in scientist discover for the first time that random “shotgun” sequencing can be applied to the whole genome with speed and accuracy. Shotgun sequencing is better known as shotgun cloning and it is used in complex long strands of the human genome. In 1996 the leaders of The Human Genome project decided that all human genomic sequence information should be free and available in a public domain within 24 hours of generation.

Read More

More about Human Genome Lab Report

Related Topics

Open Document