This addition aids in controlling the reproducibility and retention. Separation of the mixture via RP-HPLC can be done using continuous gradient or stepwise to move out the sample components. For every separation, the ideal gradient and volume must be
Pages 96-98 in Chemistry 110 Lab Manual. Wilfrid Laurier University, ON, Canada. Abstract: The purpose of this experiment was to determine the level of purity by using the values for melting point and absorbance and chemically synthesizing aspirin by using phosphoric acid as a catalyst.
Identification of an Unknown Compound using Quantitative and Qualitative Analysis Lauren Tremaglio Chemistry 1011 Lab, Section 16 Instructor: Steven Belina October 3, 2014 Our signatures indicate that this document represents the work completed by our group this semester. Experimental Design and Discussion of Results The objective of this experiment was to identify an unknown compound through quantitative and qualitative analysis. In order to find the identity of the unknown compound, an initial qualitative test for solubility was performed.
The lab specialist shall evaluate the evidence and submission information based on his/her training and experience, and shall determine which items will be analyzed. Lab specialist shall evaluate which items to analyze in a case based on several factors. These factors include nature of potential charge(s), location of items, and the nature of the item (i.e., biohazard, insufficient sample, etc.). If a case approved for analysis consists of multiple items that are all residue amounts, analysis shall be performed on at least one item. If a controlled substance is identified in the first item analyzed, no other items shall be analyzed.
In this lab, extraction was used as a method for separating a mixture of compounds. Specifically, Benzocaine and Benzoic Acid were individually isolated from a starting mixture which consisted of both components. During this lab, Benzocaine was isolated into crystals, while benzoic acid formed a white powdery solid. This was done by first isolating benzocaine. Once separated, this clear liquid had 6M of sodium hydroxide added to it and was put in an ice bath.
Further purification steps post extraction could be carried out to further increase the purity of caffeine. For example, crude caffeine could be washed again with DCM and recrystallized to further remove any impurities, which was not included in this experiment. This method of extracting caffeine ultimately produces less than ideal quantities and qualities of caffeine, and hence can be optimized to obtain purer caffeine in higher yields. The experimental design was to demonstrate the ability for DCM to extract caffeine, which supports the original hypothesis.
Aspirins can be used in tablets, capsules, and caplets. Aspirin has also been known to be used in a powdered form. Aspirin has the chemical formula of C9H8O4. Aspirin was one of the first common drugs that people used the most. 35,000 metric tons of Aspirin are produced annually, which is about 100 billion tablets a year.
In science class, we did an experiment to compare inks and their compositions. This was intended for us to learn about chromatography; the separation of a mixture through a medium (the chromatography paper) in which the components move at different rates and create different patterns. We chose to explore this topic by using four different colored markers, placing a dot of each color onto one piece of chromatography paper, and observing while the water in the beaker travels up the paper and separates the dyes. Before we started, we made a hypothesis. Mine was if paper chromatography is done on the ink in the green, red, brown, and black marker, then the green will spread out into different shades of blue and green making a pointed oval shape.
Isolation of Ecdysterone from Sesuvium portulacastrum As detailed in Figure1, Ecdysterone was isolated from Sesuvium portulacastrum using a sequential extraction process with Chloroform (CHCl3) followed by Methanol (MeOH), after which Alumina column chromatography of MeOH extract was performed. The different fractions were then eluted using varying proportions of CHCl3 and MeOH, resulting in MeOH: CHCl3 (20:80) bioactive fraction. Thin-layer chromatography (TLC) analysis of MeOH: CHCl3 (20:80) fraction was carried out using a solvent system containing MeOH (15%) and Ethyl Acetate (85%).
Aspirin as DVT Prophylaxis in Rehabilitation Inpatients Christin Porro University of Michigan Aspirin as DVT Prophylaxis in Rehabilitation Inpatients Introduction Blood clots are one of the leading causes of preventable hospital deaths in the United States. Blood clots (DVT and PE) affect upwards of 600,000 Americans each year and cause more deaths each year than the more well-publicized conditions of breast cancer, AIDS, and motor vehicle accidents ("DVT awareness," 2013, para. 1). An estimated $10 billion in medical costs in the US each year can be attributed to DVT and PE ("DVT awareness," 2013, para. 1)
And as we define HPLC (High performance liquid chromatography) and formerly known as high pressure liquid chromatography, is a classical quantitative analysis method by which we use pump in a pressurized liquid to extract the mixture and get the sample of caffeine content of soft drinks. Through this method we will able to analyze the caffeine contain. There are disadvantage and advantage of having caffeine in soft drinks by ingesting it. It brings alertness and awakens our system and repel adenosine
Detection and Purification A Monascus pigments is a complex of azaphilone compounds, which can be separated by using various analytical techniques. UV- Visible spectrophotometric methods The UV-Visible spectrophotometric method is usually used for the confirmation of pigments produced by Monascus with taking absorbance at a respective wavelength. i.e. Yellow pigment at 400, Orange 470, and Red at 500 nm.
Experiment #7: Column Chromatography of Food Dye Arianne Jan D. Tuozo Mr. Carlos Edward B. Santos October 12, 2015 Abstract Column chromatography is the separation of mixture’s components through a column. Before proceeding with the column chromatography itself, a proper solvent system must be chosen among the different solvents. The green colored food dye is the mixture whose components are separated.
Introduction: High Performance Liquid Chromatography or also known as High Performance Liquid Chromatography is one of the most powerful and most commonly used analytical separation technique. HPLC is a form of liquid chromatography that separated solutes/compounds dissolved in the solution (High-performance liquid chromatography, 2012). It is an improved form of column chromatography, where the solvent is passed through under high pressure instead of letting it drip down due to gravity. The sample is injected into the column to separate the sample of interest. The two different phases in HPLC are mobile and stationary phase.
Step 5-Data analysis: A computer based data system like the open lab chemical data system is used to process digitalized data signal and uses various mathematical calculations to determine peak areas, peak heights peak width and retention time. The chromatograph is the graphical representation of the sample elution and response complete. Each peak is identified by specific time that it leaves the column and passes through the detector called the retention time.
