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Column chromatography lab report example
Column chromatography lab report
Conclusion of column chromatography
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This addition aids in controlling the reproducibility and retention. Separation of the mixture via RP-HPLC can be done using continuous gradient or stepwise to move out the sample components. For every separation, the ideal gradient and volume must be
Pages 96-98 in Chemistry 110 Lab Manual. Wilfrid Laurier University, ON, Canada. Abstract: The purpose of this experiment was to determine the level of purity by using the values for melting point and absorbance and chemically synthesizing aspirin by using phosphoric acid as a catalyst.
The difference in this chemical and physical properties will aid in their separation. Processes like solubility, gravitational filtration and recrystallization will be used to separate the substances present in Panacetin. The melting and boiling point of the substances will help in concluding on which of these compounds will be presented at the end of experiment. Procedure and observation The Panacetin content was weighed approximately 3.0493g and transferred to the Erlenmeyer flask; 75ml of dichloromethane (CH¬2CL2) was added to the content. The dichloromethane (CH2Cl2) dissolved the sucrose, leaving the active unknown agent and aspirin behind.
Identification of an Unknown Compound using Quantitative and Qualitative Analysis Lauren Tremaglio Chemistry 1011 Lab, Section 16 Instructor: Steven Belina October 3, 2014 Our signatures indicate that this document represents the work completed by our group this semester. Experimental Design and Discussion of Results The objective of this experiment was to identify an unknown compound through quantitative and qualitative analysis. In order to find the identity of the unknown compound, an initial qualitative test for solubility was performed.
The lab specialist shall evaluate the evidence and submission information based on his/her training and experience, and shall determine which items will be analyzed. Lab specialist shall evaluate which items to analyze in a case based on several factors. These factors include nature of potential charge(s), location of items, and the nature of the item (i.e., biohazard, insufficient sample, etc.). If a case approved for analysis consists of multiple items that are all residue amounts, analysis shall be performed on at least one item. If a controlled substance is identified in the first item analyzed, no other items shall be analyzed.
Further purification steps post extraction could be carried out to further increase the purity of caffeine. For example, crude caffeine could be washed again with DCM and recrystallized to further remove any impurities, which was not included in this experiment. This method of extracting caffeine ultimately produces less than ideal quantities and qualities of caffeine, and hence can be optimized to obtain purer caffeine in higher yields. The experimental design was to demonstrate the ability for DCM to extract caffeine, which supports the original hypothesis.
Leah Romero 10/30/2017 Conclusion Lab 3 Chem 102L In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes.
The melting point of the experimentally synthesized Aspirin product was found to be between 126-129 ˚C. This temperature range of initial to final melting point has a small and sharp temperature range of only 3˚C, which is within the acceptable limits of the 128-137˚C1 literature value for Aspirin (Acetylsalicylic Acid/2-acetoxybenzoic acid), if located slightly toward the beginning of the literature melting temperature range. Therefore, the narrow melting point range, which falls within the standard literature value range results, indicate the reliability purity of the sample. Had the melting point been higher than the literature value, but maintained a sharp melting point range, the compound could have still indicated a pure sample. A lower
Aspirins can be used in tablets, capsules, and caplets. Aspirin has also been known to be used in a powdered form. Aspirin has the chemical formula of C9H8O4. Aspirin was one of the first common drugs that people used the most. 35,000 metric tons of Aspirin are produced annually, which is about 100 billion tablets a year.
In science class, we did an experiment to compare inks and their compositions. This was intended for us to learn about chromatography; the separation of a mixture through a medium (the chromatography paper) in which the components move at different rates and create different patterns. We chose to explore this topic by using four different colored markers, placing a dot of each color onto one piece of chromatography paper, and observing while the water in the beaker travels up the paper and separates the dyes. Before we started, we made a hypothesis. Mine was if paper chromatography is done on the ink in the green, red, brown, and black marker, then the green will spread out into different shades of blue and green making a pointed oval shape.
Isolation of Ecdysterone from Sesuvium portulacastrum As detailed in Figure1, Ecdysterone was isolated from Sesuvium portulacastrum using a sequential extraction process with Chloroform (CHCl3) followed by Methanol (MeOH), after which Alumina column chromatography of MeOH extract was performed. The different fractions were then eluted using varying proportions of CHCl3 and MeOH, resulting in MeOH: CHCl3 (20:80) bioactive fraction. Thin-layer chromatography (TLC) analysis of MeOH: CHCl3 (20:80) fraction was carried out using a solvent system containing MeOH (15%) and Ethyl Acetate (85%).
Aspirin as DVT Prophylaxis in Rehabilitation Inpatients Christin Porro University of Michigan Aspirin as DVT Prophylaxis in Rehabilitation Inpatients Introduction Blood clots are one of the leading causes of preventable hospital deaths in the United States. Blood clots (DVT and PE) affect upwards of 600,000 Americans each year and cause more deaths each year than the more well-publicized conditions of breast cancer, AIDS, and motor vehicle accidents ("DVT awareness," 2013, para. 1). An estimated $10 billion in medical costs in the US each year can be attributed to DVT and PE ("DVT awareness," 2013, para. 1)
figure 2.1 (The HPLC system). We start in the mobile phase and ends to a detector where in these detectors is connected to a computer. Then this computer it in a graph (see figure 2.2) to show our sample soft drinks caffeine level content which has a chemical name of
Experiment #7: Column Chromatography of Food Dye Arianne Jan D. Tuozo Mr. Carlos Edward B. Santos October 12, 2015 Abstract Column chromatography is the separation of mixture’s components through a column. Before proceeding with the column chromatography itself, a proper solvent system must be chosen among the different solvents. The green colored food dye is the mixture whose components are separated.
Step 5-Data analysis: A computer based data system like the open lab chemical data system is used to process digitalized data signal and uses various mathematical calculations to determine peak areas, peak heights peak width and retention time. The chromatograph is the graphical representation of the sample elution and response complete. Each peak is identified by specific time that it leaves the column and passes through the detector called the retention time.
