By completing this experiment, knowledge collected about optimal pH in enzymes will help
The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.
Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable temperature 3. Controlled Variables pH, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Substrate Concentration on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.
At last, at 95°C, or 85ºC, the enzyme activity is showed at being in its greater temperature. From black at 0 minutes, the color became bright yellow at 10 minutes, which specify the optimal temperature for
In this lab we used mechanical weathering to shake up sugar cubes inside of a mason jar. To complete the lab we needed to shake for 3 minutes to see how the mechanical weathering actually works. The sugar cubes represented the rocks and minerals. We used sugar cubes because they have a mass less than other rocks and minerals. I thought as we shook the cube it would just crumble into little bits.
LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.22.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3. Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.
Enzymes speed up chemical reactions enabling more products to be formed within a shorter span of time. Enzymes are fragile and easily disrupted by heat or other mild treatment. Studying the effect of temperature and substrate concentration on enzyme concentration allows better understanding of optimum conditions which enzymes can function. An example of an enzyme catalyzed reaction is enzymatic hydrolysis of an artificial substrate, o-Nitrophenylgalactoside (ONPG) used in place of lactose. Upon hydrolysis by B-galactosidase, a yellow colored compound o-Nitrophenol (ONP) is formed.
In Part A, students would observe the effects of enzyme concentration on the rate of reaction. They were required to make six solutions with different concentration of catalase, the speed of
After record your data and determine the absolute rate of the enzyme-catalyzed reaction. Based on the data and observations the hypothesis was accepted. It was accepted because when pH were changed to a variety of levels the transmittance began to get higher reaction rates. The increased absorbance means greater amount of product and a higher reaction rate will be produced.
Every concentration (0, 5, 10 and 20%) reached its saturation point. The 10% salt solution displayed the steadiest curve of all concentrations. Of the four concentrations, 10% and 20% salt concentrations show the smoothest curves and lowest absorbance. Of the four different concentrations, the 10% concentration is optimal for enzyme activity compared to the lower concentrations. We reject the null hypothesis, because the higher salt concentrations are, the slower the rate of an enzyme reaction will become.
RESULTS In my experiment I found that the realtivity between densities and temperature corispond to each other. I first found the densities of all 5 liquids.. I first found how much my cylinder that i was going to measure my liquids in weighs. I did this because when i go to measure my other liquids i have subtract the weight of the cylinder to get an accurate measurement for the liquid.
For the first experiment, we found our hypothesis to be supported by our results. We hypothesized that water would be have the most physical change in solute. According to the class data, water was able to dissolve magnesium sulfate, glucose, sucrose, and salt into true solutions. However, for pod six they claimed that their mixture for water and sucrose was more suspension than a true solution. Factors that may contribute to this answer can vary from the how well did they mix their mixture, how long did they mix it for, or their personal perception of what they believe the mixture is classified as.
Moving forward, to find the volume of the metal object, the graduated cylinder was filled with water. The initial volume in the graduated cylinder read 21.0 mL. The metal object was then placed in the graduated cylinder carefully, as to not splash water. In the graduated cylinder of 21.0 mL, the metal object raised the water to 24.1 mL, thus the object was 3.1 mL for measurement 1. In a graduated cylinder of 21.0 mL, the metal object raised the water to 24.0 mL, thus the metal object was 3.0 mL for measurement 2. In a graduated cylinder of 21.0 mL, the metal object raised the water level to 23.9 mL, thus the object was 2.9 mL for measurement 3.
By observing figure 3, the more enzyme that is available, the faster the reaction rate is. The optimal enzyme concentration was chosen based on the R2 values from figure 2. The highest observable rate also had the best R2 number, which was closest to one. This enzyme concentration was used in part 2.
Density: A Characteristic Property I Purpose The purpose of this experiment is the density of six different substances. The way to figure this out is by finding the mass and volume of each of the substances.
