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Dna Synthesis Lab Report

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Purpose: DNA, also known as deoxyribonucleic acid is found in all living organisms. It was what holds genetic information. DNA is a genetic code that is composed of four components adenine, guanine, cytosine, and thymine also referred to as bases. These components make-up DNA and form a double through the base pairing of adenine to thymine and guanine to cytosine. Adenine is a purine nucleobase, a six-membered nitrogen ring fused to a five-membered nitrogen ring. Adenine provides stability to the double helix when it pairs to it base pair thymine. Guanine is also a purine. It binds to cytosine with 3 hydrogen bonds. Cytosine is a pyrimidine. It has only one ring, unlike adenine and guanine which have two. Cytosine only attaches to guanine but it has three bonding sites. Thymine also like cytosine in a pyrimidine but unlike the rest of the bases, thymine is not found in RNA. In RNA it is replaced by uricel. In the experiments 14-16 in this lab, we tested our own DNA from our cheek cells to find out if we were heterozygous or homozygous. Every person has variations in there DNA. We explored the DNA of chromosome 8. We used Alu …show more content…

Chelex solution binds to cations including mg2+ for the preparation of DNA extraction. Master Mix contains all the materials except for DNA and primers for PRC. Primer Mix is used to determine the DNA segment that will be amplified. Positive Control DNA is a mix that should work in the in normal conditions. It shows that there isn't any problem with the Master Mix. Negative Control DNA is used to make sure there is no contamination and no amplicons should present. It lacks a DNA template. Thermocycler is used to amplify DNA segments and to change temperatures for annealing, denaturation, and extension. Agarose Gel is a cast made of agarose used to separate mixed DNA. DNA Ladder is used as a marker used to find unknown DNA

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