ipl-logo

Enzyme Lab Report

1377 Words6 Pages

The aims of this practical are to examine the effects of various substances on the activity of glycogen phosphorylase by the principles of allosteric control of the enzyme and reversible phosphorylation. These principles aim to reverse the effect glycogen phosphorylase has on the conversion of glycogen to glucose-1-phosphate, i.e. causing glycogen and glucose-1-phosphate to bind and release a phosphate. The amount of phosphate formed in this experiment is measured by the principles of a spectrometry reading at 660nm. [https://www.ncbi.nlm.nih.gov/books/NBK22354/]] Introduction: Glycogen is used in animals as a form of glucose that can be kept in storage in cells until there is a diminished amount of glucose in the body, which then glycogen …show more content…

Explain why samples 1-3 should all have a high concentration of phosphate, ie high A660 nm values. Comment on any deviation of your results from the expected outcome. Samples one to three should have a high concentration of phosphate as all the tubes contained ATP and Buffer A. Tube one and two contained the kinase, and tube one and three contained phosphorylase A. Phosphorylase kinase activates glycogen phosphorylase [berg] and because the kinase and phosphorylase A, the active phosphorylase, are both present in tube one, tube one is expected to have a high amount of phosphate present as the reversible phosphorylation can occur at a high rate. This is reflected in the actual results as the absorbance reading at 660nm for tube one is 1.237. Tube two also has a high expected and actual absorbance reading as phosphorylase kinase is present in the tube. Tube two absorbance reading at 660nm is 0.5835. However, the reading in tube two is not as high as the other tubes, tube one and three, but that is due to the less active form of phosphorylase, phosphorylase B, being present in the tube. The time taken for phosphorylase kinase to convert phosphorylase B to its active form, phosphorylase A, might have caused the absorbance reading to be lower. [berg] Sample three had a absorbance reading at 660nm at 1.29. This is due to the fact the tube contained the active form of phosphorylase, phosphorylase A, in the sample which helps breakdown glycogen.*****

Show More
Related

Lab Report Organic Compound Catecholase

805 Words | 4 Pages

The control tube is tube number three because it contained the same ingredients as that of the first experiment where we determined that when catechol reacts with potato extract it turns brown. In test tubes one and two a different amount of catechol was added. This is because we needed to determine what kind of inhibitor the PTU was. Because there was no change in color with either test tube one or two, we found that PTU was a non-competitive inhibitor.

Read More

Lab Report On Enzyme Activity

1419 Words | 6 Pages

Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable pH 3. Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity 1.

Read More

Mitochondria Lab Report

737 Words | 3 Pages

Although the overall absorbance increases as more milliliters of mitochondrial suspension is added to a mixture of 0.25 mL of 0.5 mM DCIP, 0.5 mL of 50 mM sodium azide, various volumes of assay buffer (20 mM potassium

Read More

Laboratory Report on Enzyme Activity

1407 Words | 6 Pages

55 degrees celcius Table 6: Effect of Sucrose Concentration on Sucrase Activity Optical Density 35 g/L 30 g/L 25 g/L 20 g/L 15 g/L 10 g/L 5 g/L 0 g/L 1 1.007 0.974 0.950 0.926 0.849 0.734 0.515 0.003 2 1.002 1.011 0.947 0.937 0.834 0.766 0.496 0.002 3 0.980 0.998 0.944 0.932 0.838 0.754 0.495 0.001 average 0.996 0.994 0.947 0.932 0.840 0.751 0.502 0.002 Effect of Sucrose Concentration on Sucrase Activity 5. State how sucrase activity changes with increasing sucrose concentration. First sucrase activity increases greatly. After 10 g/l sucrase activity continues to increase but at a slow rate until it reaches 30 g/l. At 30 g/l to 35 g/l sucrase activities mostly stayed the same

Read More

B-Galactosidase Lab Report

1764 Words | 8 Pages

B-galactosidase breaks down the disaccharide lactose into simple sugars glucose and galactose. However, glucose is a colorless compound hence it has to be substituted with a compound that is detectable by a visible color change. Hence,

Read More

Measure The Mass Of Sucrose Solution Lab Report

1258 Words | 6 Pages

Procedure Activity 1: Fill 6 large beaker halfway with distilled water, making sure all beakers have equal amounts of water. Cut 6 30 cm dialysis bags and label each bag with a letter, A through F. Fill each dialysis bag with 15 mL of solution A through F that corresponds with the lab on each bag. For example, bag A is filled with solution A. Measure the mass of each dialysis bag and record masses of each bag in BILL. Cover the beakers with paper towel and leave the bags in the beakers overnight. Remove the dialysis bags from the beakers and let dry.

Read More

Bovine Serum Albumin Protein Lab Report

1092 Words | 5 Pages

This reaction results in a product with a blue color, and in order to determine the protein amount in each sample, it is necessary to read its absorbance at 660nm and compare that against a standard curve of Bovine Serum Albumin. It is essential to incorporate the color product of the reaction that reduces the Folin phenol reagent, as the spectrophotometer does not have the ability to detect and uncolored compounds

Read More

Amylase Temperature Experiment

924 Words | 4 Pages

(A for amylase solution) e. Label the other 5 test tubes S1-S5 (S for starch solution) f. Place all the test tubes in a test tube rack g. Label one beaker “cold water” and fill it with cold water from the water dispenser h. Label another beaker “normal water” and fill it with the normal water from the dispenser i. Label another beaker “ warm water and fill it with hot water from the water dispenser j. Label another beaker “ very warm water and fill it with hot water from the water dispenser, then place it on magnetic stirrer (hot plate stirrer) while measuring its temperature k. Label another beaker “ hot water and fill it with hot water from the water dispenser, then place it on magnetic stirrer (hot plate stirrer) while measuring its

Read More

Eosine And Metolythene Essay

1410 Words | 6 Pages

Obtain one tube for each sugar, usually one for glucose, lactose, sucrose, mannitol.   Do not get them confused, they look the same, it is suggested that they be labelled immediately if they are not already labelled. 2. Observe the tubes before you begin.   They should have an inverted Durham tube that is filled fully with broth (they are to test for gas production).

Read More

Enzyme Design Lab

361 Words | 2 Pages

The longer it takes for the substrate to be broken down. Test Tube One only had 0.5 milliliters of lactase, while Test Tube Four had 5 milliliters of glucose. Test Tube One for both trials had a 1 /10 of glucose, while Test Tube Four had 1/2 glucose. So you can see a correlation with the amount of enzymes. We took note that for Test Tube Four

Read More

Naproxen Essay

2661 Words | 11 Pages

g/ml) Absorbance (nm) 1 0 0 2 2 0.078 3 4 0.161 4 6 0.224 5 8 0.295 6 10 0.384 7 12 0.443 Figure 6: Calibration Curve of Naproxen in

Read More

Yeast Fermentation Report

1893 Words | 8 Pages

The tube was closed in one end and the test tube was put into a tub with hot water, at about 55 degrees Celsius. The test tube was then filled with water and the base was left empty to mark a baseline. The test tube was then put in a beaker with hot water. In another test tube different concentrations of yeast and corn were mixed. The combinations included 2× yeast; 1× sugar, 1× yeast; 1× sugar and others.

Read More

Alkaline Phosphatase Lab Report

1627 Words | 7 Pages

The temperature of the SPM gets increased by 1oC for every test tube solution, until test tube 7 with an SPM temperature of 40oC. After all the absorbencies for the varying temperatures had been recorded – the product concentration of each test tube solution was calculated using the absorbency readings at 10 minutes for each respective test tube mixture. The product concentration was calculated using Beer-Lamberts’ law of A = ECL. A graph plotting the product concentration against their corresponding temperatures was then drawn up, the peak of the graph being the optimal

Read More

Glycation Lab Report

802 Words | 4 Pages

ADVANCED GLYCATION END PRODUCTS Advanced glycation end product as an oxidative process that occurred in food perserved or heated the presence of reducing sugars were first observed in early 1900s which non-enzymatically make the food proteins insoluble with yellowish fluorescent products attached to them. This process is known as Maillard reaction named on its discoverer the Louis C. Maillard in 1912 (Finot ., 2005). The Maillard reaction is the one of physiological significance. In vivo, it is also observed that human protein non-enzymatically glycated in the presence of physiologic sugars. One of the best characterized glycation product known to occur in the body is glysocylated hemoglobin.

Read More

Reducing Sugar Lab Report

1083 Words | 5 Pages

The difference in pH in fructose-glycine of pH5 is negligible but there is a difference in pH in fructose-glycine of pH8. This happens because the fructose sugar degrades more rapidly than the amino acids. At the same time, glycine was moderately lost below pH8. The final value of pH is dependent on the formation and degradation of sugar and amino acid.

Read More

More about Enzyme Lab Report

Open Document