ipl-logo

Enzyme Lab Report

1150 Words5 Pages

The data received in the experiment only somewhat supported my hypothesis. My group tested how changing the pH of the hydrogen peroxide substrate would affect enzyme activity and the rate of reaction. Our hypothesis was that the solution with a pH closest to the pH of hydrogen peroxide, which is fairly acidic, would have the fastest rate of reaction. After conducting the experiment, we found that an acidic pH of 2 resulted in the reaction having a reaction rate of 0 sec-, while when conducted with a substrate pH of 7, the reaction proceeded at the fastest rate of 0.051 sec-. These results show that a pH of 7—a more neutral solution—actually caused the enzyme to work the best, as opposed to the predicted acidic substrate. While the data didn’t …show more content…

Since enzymes are extremely specific, the hydrogen peroxide can only bind to catalase because of the specific shape of catalase’s active site. If the shape of catalase is changed, then the binding of the hydrogen peroxide will be decreased, or nonexistent in the case that the catalase shape is changed so much that the tertiary structure unravels, and the enzyme becomes denatured. One factor that can cause the enzyme to change shape is pH. A acidic pH means that there are excess H+ ions in the solution, while a basic pH means that the solution contains excess OH- ions. These ions can interact with the R-groups in the enzyme and cause the shape to change. In the experiment, the optimal pH of 7 means that since its neutral there are very little ions in the solution, and the ions that are in the solution reacts with the R-groups in the catalase to actually cause the hydrogen peroxide to fit in catalase’s active site better. When the pH was decreased or increased past 7, then the excess ions changed the shape of the active site too much and the hydrogen peroxide couldn’t bind as well. Furthermore, when the pH was way too acidic or basic, like with the pH of 2, the excess ions caused the enzyme to denature, thus resulting in the reaction rate of 0

Show More
Related

Turnip Peroxidase Lab Report

881 Words | 4 Pages

As pH increases or decreases to get closer to the optimal pH --in this case it is 7 for this particular enzyme-- the rate of reaction peaks and is highest at that point, which is described by the molecular shape and structure of the enzyme at its optimal pH. When turnip peroxidase is at pH 7, the active site is able to fit perfectly with the substrate, therefore explaining why the reaction rate is fastest at this point. Accordingly, if the active site is disrupted, the substrate cannot fit perfectly causing the reaction rate to slow down. This can be supported by the data because the reaction rate gradually increased from pH 3 to pH 7 and reached its maximum at pH 7. Once it did reach the optimal pH, the reaction rate continuously decreased

Read More

Ph Levels Lab Hypothesis

421 Words | 2 Pages

The purpose of this project is to further test the effect of pH levels, and how they affect the production of enzymes. The hypothesis for this experiment was the more basic the ph buffer the high the activity of the enzyme. The more acidic the pH buffer the less activity of the enzyme. The first time the experiment was done with only three pH levels that were tested. That helped find the range of pH levels that were needed to test the second time.

Read More

Turnip Peroxidase Lab Report

2136 Words | 9 Pages

The objective of this lab was to determine the best pH level to increase enzyme activity. As this objective was met, it was discovered that water (pH level 7) was the best for percent absorbance. The hypothesis for this experiment was, “If peroxidase is an enzyme and therefore contains certain pH tolerances, then when placed in solution with pH levels of three, seven, and ten and the reaction is measured by a colorimeter, then water will be the optimal solution for maximum reaction rate.” As seen in the tables and graphs, the data supported the hypothesis due to the fact that most enzymes have an optimal pH of 4-9.

Read More

Enzyme Turnip Peroxidase Lab Report

1117 Words | 5 Pages

The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.

Read More

Horseradish Peroxidase Lab Report

1090 Words | 5 Pages

It was hypothesized that the optimal pH for the enzyme was pH 7 while the 1.0 ml peroxidase would have the best reaction rate. At the end of the experiment the results prove the hypothesis to be incorrect. INTRODUCTION Enzymes are proteins that allow a reaction to speed up. These proteins are made up of monomers known as amino acids.

Read More

Catabolic Reaction Experiment

1060 Words | 5 Pages

7. Will the temperature effect the pH Scale? Hypothesis: The hypothesis of this experiment is that the rate of reaction will increases well as the hydrogen peroxide concentration. If the temperature, pH and enzyme concentration is kept constant then the rate of reaction will start to decrease as well as the hydrogen peroxide concentration. Aim: To investigate the effects of changing the concentration of the enzyme catalase that it has on the rate of breaking down the Hydrogen Peroxide solution.

Read More

Catalase Lab

1528 Words | 7 Pages

molecules produced per unit time). In order to do so, enzymes bind temporarily to one or more reactants, lowering the amount of activation energy needed and thus speed up the reaction. Most of the cells use an enzyme called catalase to reduce the amount of hydrogen peroxide, a toxic substance that could accumulate as a result of normal cell activity. The purpose of this experiment was to observe the effects that enzyme concentration and copper (II) sulphate would have on the rate of reaction. This experiment was divided into two parts: Part A and Part B.

Read More

Enzyme Lab

901 Words | 4 Pages

After record your data and determine the absolute rate of the enzyme-catalyzed reaction. Based on the data and observations the hypothesis was accepted. It was accepted because when pH were changed to a variety of levels the transmittance began to get higher reaction rates. The increased absorbance means greater amount of product and a higher reaction rate will be produced.

Read More

Peroxidase Lab Report

226 Words | 1 Pages

In this lab we determined the effect of PH after their mixing of the substances to get the result of the peroxide strength or weakness during the experiments of Spectrophotometer by using test tubes and absorbance V’s time activities over Enzyme concentration, PH, Temperature, and inhibitors on the Peroxidase reaction. A future experiment might include an additional calibration using the new and advanced Spectrophotometer soft wear and others technologies such as the reactions of the compounds with Peroxide and other materials to further test the nature of the PH and more positively or negatively we will identify the PH effect on Enzyme. Increasing the temperature increases reaction rates because of the disproportionately large increase in

Read More

Enzyme Activity Lab Report

720 Words | 3 Pages

How does it affect the enzyme Activity?: The value of pH is from 1 to 14 and pH can be neutral,alkali or acidic.pH 7 shows that it is netural and any number higher than pH7 is alkaline and lower than pH7 is acidic.pH are concentration of hydrogen ions that are found in solution. However, there is more concentration of hydrogen ions (protons) in acidic solutions and they are known as proton donors. If we look at the strucutre of the enzymes especially tertiary strucutre you can find bonds such as hydrogen bond and lots of ionic , which gives the shape to the active site and helps to keep them in place properly. These enzyme are made out of amino acid and within them you can find oppositely charged group that are attracted to each other , which

Read More

Catalase Lab

246 Words | 1 Pages

In conclusion, the data from the experiment supports my hypothesis that catalase will function the most efficiently at a neutral pH of 7. As the pH of the solutions decreased, so did the amount of hydrogen peroxide consumed. Furthermore, after calculating the reaction rates, it is shown that the reaction rate in the solution with a pH of 7 (.5 mm/sec) was higher than any other solution. By looking at the graph above, you can see that as the pH of the solution rose to a pH of 7, catalase became more efficient and was able to better carry out its function. These results help support the idea that as a solution becomes more acidic than the optimum pH of an enzyme, the enzymes present in the solution will denature, and in turn will not be

Read More

Lab Report On Catalase And Temperature

899 Words | 4 Pages

H20 + 2 O2 This experiment will use 1% catalase solution and 3% hydrogen peroxide solution, both diluted into water so the reaction slows down. Temperature will be controlled in this experiment to change the reaction speed of the enzyme and the substrate, this is what the experiment is looking at. The effect of the temperature will be determined by how much gas is released in two minutes, which will change the pressure inside the test tube and will be measured by a gas

Read More

Potato Enzyme Lab Report

1182 Words | 5 Pages

Introduction In class, a series of experiments were performed that pertained to the enzyme known as catalase, which converts hydrogen peroxide into oxygen. Due to peroxide being toxic to the tissues of both plants and animals, both possess the enzyme catalase, which breaks into two non-toxic compounds: water and oxygen gas. Enzymes are proteins that react to certain substrates to create a product, and continue doing so afterwards. Methods and Materials To test reactions between catalase and hydrogen peroxide, groups of three to four people were formed.

Read More

Bio Chem: Lab Report Of Amylase In Enzyme

916 Words | 4 Pages

In this experiment , we can prove that the temperature, pH and salt are the factors that will affect the structure and function of the enzyme as it is a kind of protein . Therefore, there may be an influence on the activity of enzyme which substrates cannot be binded on the active site if the amylase in too high or low ph and temperature and excess salt environment . On the other hand optimum ph and temperature and suitable salt concentration may favour the amylase activity . Reference : 1.2016, May 08). Effects of pH on Amylase Activity.

Read More

The Effect Of Temperature On The Activity Of A Potato Lab

3027 Words | 13 Pages

This happens because enzymes lower the activation energy, as they provide an alternative reaction pathway. The decrease in the energy level aids in making the process happen faster (Jae In Lee, 2011) A catalase is an enzyme, which is found in all living organisms. This enzyme helps to convert hydrogen peroxide into oxygen and water. Chemical actions that happen within the cell produces hydrogen peroxide, which is poisonous and therefore can kill the organism.

Read More

More about Enzyme Lab Report

Open Document