Enzyme Peroxidase Lab Report

Abstract In this experiment it was examined whether the enzyme peroxidase will work fastest in a pH of 8.0. We placed the enzyme peroxidase in a reaction with guaiacol and hydrogen peroxide in four different pH solutions. Then recorded the absorbencies for each reaction until all substrates were used up, and calculated the initial reaction velocities for each. We found that the reaction in a pH 7.0 solution had the highest initial reaction velocity. Over-all this study shows that the enzyme peroxidase will work the fastest in a 7.0 solution.
Introduction
Enzymes are useful catalytic proteins that are needed in life that help with both the absorption of nutrients and break them down. They have appeared in both RNA and DNA which means that

Test tube 1 contained water, test tube 2 contained pH 4.0, test tube 3 contained pH 7.0, and test tube 4 contained 8.0. A peroxidase enzyme solution was prepared by taking about 2 grams of turnip, cut from the inside, and placing it in a blender with 150 milliliters of deionized water. The final mixture was poured through a coffee filter to make sure there were no large lumps in the mixture. The first test tube received 300 micro liters of guaiacol and 300 micro liters of hydrogen peroxide both measured and dispensed using a micropipette. A pipette was filled with 1 milliliter of the peroxidase enzyme solution that was created earlier and placed in the test tube. The test tube was quickly inverted once or twice and then poured into the cuvette and placed into the calorimeter. The absorbency readings were recorded every fifteen seconds for about five minutes, or until the reaction was complete. The same steps were followed for each of the test tubes. We then calculated the initial reaction velocity for each of the different pHs using the following equation (absorbency as y and time as

In test tube 2, when pH 4.0 solution was mixed with the reactants this reaction reached completion at 225 seconds. When pH 7.0 solution reacted with the reactants its reaction went to completion at 180 seconds. Finally, when pH 8.0 solution was mixed with the reactants its reaction came to completion at 210 seconds (Figure 1). Test tube 3 was filled with a pH 7.0 solution had the highest initial reaction velocity followed by test tube 4 containing a pH 8.0 solution. Test tube 2 had the pH4.0 solution which was second to last for the IRV calculation, and finally the base line test tube had the lowest IRV (Table