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Ninhydrin Lab Report

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MSc FOOD BUSINESS MANAGEMENT AND TECHNOLOGY Food Formulation & Processing Laboratory Practical Submitted to, Submitted by, Janis Kelly Blesson Abraham Mathew PRACTICAL NO- 3 SEPARATION OF AMINO ACIDS BY THIN LAYER CHROMATOGRAPHY Chromatography Chromatography is a technique that is used to separate two or more different …show more content…

(b) attractions between the compound and the silica, the more the compound interacts with silica, the lesser it moves, (c) size of the compound, the larger the compound the slower it moves up the plate. As the solvent slowly travels up the plate, the different components of the dye mixture travel at different rates and the mixture is separated into different coloured spots (in case of using a coloured mixture). This leads to the development of TLC plate. The plate is removed after an optimal development time and dried and the spots/zones are detected using a suitable location reagent (in case of separation of colourless compounds, Ninhydrin is used in this case which helps in developing colour). An important characteristic used in thin layer chromatography is Rf value. Fig .1: Technique for TLC chromatography Chromatographic Separation of Amino acids The experiment aims to bring about separation of a mixture of amino acids using the technique of thin layer chromatography to separate the amino acids in a given mixture. There are 20 standard amino acids which are a-amino acids. They are made up of two groups and

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For most sequences at position 4 and 5 we observe only the nucleotides G and T, respectively. There may be rare cases where other nucleotides may also be found. To consider such observations, we need to do a process called additive smoothing or Laplace smoothing to smooth the categorical data. [9] In this case, we add 4 sequences: AAAAAAAAA, CCCCCCCCC, GGGGGGGG, TTTTTTTTT.

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I need to find the area of rectangle ABCD. I know that ABCD is a rectangle with diagonals intersecting at point E. Segment DE equals 4x-5, segment BC equals 2x+6, and segment AC equals 6x. I predict that To find the area of rectangle ABCD I need to find out the base and height of the rectangle. The first step is to find what x equals. Since I know the intersecting line segments AC and DB are congruent that means when I times the equation 4x-5 for segment DE by two it will equal the equation 6x for segment AC.

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LABORATORY REPORT EXERCISE #5 INTRODUCTION TO THE COMPOUND LIGHT MICROSCOPE, PLANT AND ANIMAL CELLS Name_______________________________Section_____Teacher______________Date________ PRE-LAB QUESTIONS - answer the following questions using your textbook and valid internet sources. Be sure to cite your sources at the end of the prelab. You can type your answers to all questions except #1 and #9 directly into this document and then submit via Canvas. Type the answers for #1 and #9 at the end of the document. 1.

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Suppose we have a single-hop RCS where there is one AF relay that amplifies the signal received from a transmitter and forwards it to a receiver. Assume that the transmitter sends over the transmitter-to-relay channel a data symbol ${s_k}$, from a set of finite modulation alphabet, $S={S_1, S_2,ldots,S_{cal A}}$, where ${cal A}$ denotes the size of the modulation alphabet. The discrete-time baseband equivalent signal received by the relay, $z_k$, at time $k$ is given by egin{equation} z_k = h_{1,k}s_k + n_{1,k},~~~~for~~k=1,2,ldots,M label{relaySignal} end{equation} where $n_{1,k}sim {cal N}_c(0,sigma_{n1}^2)$ is a circularly-symmetric complex Gaussian noise added by the transmitter-to-relay channel, $h_{1,k}$ denotes the transmitter-to-relay channel, and

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determine each pixel belongs to background or foreground. Wis the weights between the pattern and summationneurons, which are used to point out with which a pattern belongs to the background or foreground. They areupdated when each new value of a pixel at a certain position received by implementing the following function:Wt+1ib =fc(1−βNpn)Wib+MAtβ!(37)Wt+1i f=(1−Wt+1ib)(38)whereWtibis the weight between theith pattern neuron and the background summation neuron at timet,βisthe learning rate,Npnis the number of the pattern neurons of BNN,fcis the following function:fc(x)1,x>1x,x≤1(39)MAtindicates the neuron with the maximum response (activation potential) at frame t, according to:MAt1,f or neuron with maximum response0,otherwise(40)Function

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This experiment aims to separate the components of the green colored food dye and get the TLC profile of each eluent collected. III. Experimental Procedure Before starting with the column chromatography for food dye, the right solvent must be chosen between 2-butanol with acetic acid, ammonia in butanol, 1 part 1-butanol 1 part acetic acid, and 2 parts methanol 1 part water. In choosing the appropriate solvent for column chromatography, the solvent system must give a TLC profile wherein most of the spots are well separated and has a Rf value within 0.3-0.5.

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