The transposon of Tn4351 was originally detected in E. coli which carried an unstable chimeric plasmid, pSS-2. The mobilization of pSS-2 from onestrain of E. coli
Instructions: Answer the questions as directed. Upload the assignment prior to the beginning of your next lab section. Make sure you give yourself time to troubleshoot any issues you may have with uploading the assignment. You are responsible for uploading the correct file. Given the map of the plasmid in Figure 10-3, you should be able to predict the length of DNA fragments that will result when these digests are completed.
The same region is also amplified on both chromosomes, however they are different sizes, which are then put into gel
The Solid sequencing platform, produced by Technologies/Applied Biosystems (ABI), performs sequencing by ligation method. Similar like the Roche 454 library preparation, genomic double strand DNA were sheared into small pieces and ligated with two types of adatptors P1 and P2 on two ends. One end with P1 adaptor binds onto the surface of the magnetic bead and emulsion PCR takes place to amplify single nucleotide fragment. Then the oil was washed out and four fluorescent labeled di-bases probes were added into the beads mixture. By matching the 1st and 2nd position of the template by di-base probes, fluorescence was detected and the extra tail with fluorescent probe is cleaved out.
Q1A: What is the mechanism of action of colistin? Colistin is an antibiotic that works best against Gram-negative bacteria. It works by binding to LPSs (lipopolysaccrides) and phospholipids in the outer cell membrane of the bacteria. This, in turn, disrupts the outer cell membrane by displacing cations and leaking the intracellular contents, combining it with outer cellular contents, causing the bacteria to be unable to differentiate the bacteria’s intra and outer cellular contents from one another.
The DNA is loaded into wells in the gel that are made when creating the gel. Since DNA is negatively charged it will repel the negative charge in the gel box, and move towards the positive end. This will separate the bands to make a pattern. Then the pattern created will be used to analyze other DNA samples to find the suspect. If every single band matches between 2 DNA well tests, that means that they came from the same person.
On the second day after these colonies were grown, the lab begun by labeling two test tubes -DNA and +DNA, adding the transformation solution to to each, and placing it on ice. Then the procedure called for the use of a sterile loop to pick up a colony of E coli and add it to each test tube, then after E coli was in both test tubes, the pGLO DNA was placed in ONLY the +DNA test tube. After 10 minutes on ice, and after the 4 plates are labeled each to LB -DNA, LB/amp -DNA, LB/amp +DNA, and LB/amp/ara +DNA respectively, the test tubes are heat shocked to loosen the DNA cell membrane to allow pGLO to slip in and code within the DNA. After more time in the ice bath, the tubes are taken out and set at room temperature with the LB broth placed in both test tubes. Then the dishes LB/amp +DNA and LB/amp/ara +DNA were spread with the +DNA solution, and the dishes LB -DNA, and LB/amp -DNA were spread with the -DNA solution.
DNA Fingerprinting Using Agarose Gel S. Aaron Sowards Bio 122 Lab 04 Brianna Adanitsch Jakob Lester Minhenga Ngijoi 2/21/18 Dr. Chad R. Sethman Abstract DNA fingerprinting is the process of analyzing an individual’s DNA base-pair patterns. The DNA fingerprinting lab involved identifying the suspect using Agarose Gel and Polymerase Chain Reaction. It was found that suspect two s DNA matched the crime scene DNA.
Gel Pro The mats of Gel Pro were created with one single aim in mind: to make the time you use standing more relaxed and pleasurable. It takes pride in being the experts of pioneering anti-fatigue comfort mats for homes and businesses. It all began with a Thanksgiving dinner. Lisa McMahan shuffled her feet from side to side on the cold hardwood kitchen floor.
GELO 2 F17 1. Based on the graph, there is a correlation between the effects of CO2 concentration and the average global temperature. They both resulted in an exponential growth over the period between 1880 to 2010 relatively with the same amount of increase. While the global average temperature from 1880 to 2010 gradually increased relatively from 56.5 to 58.1 Fahrenheits, the amount of CO 2 Concentration (ppmv) also increased as well ranging from 280 to 390. This is an example of science because it uses properly considered and provided all evidence shown. Although the data have many ups and down trends of different temperature and climate changes within the years, it also shows the gradual increase of temperature over the years.
Usually, the microbial enzymes have various potential uses in industries and medicine. The microbial enzymes are also more reliable than plant and animal enzymes as they are more stable and active. Also the microorganisms demonstrate an alternative source of enzymes because they can be cultured in large quantities in a short time by fermentation and owing to their biochemical diversity and susceptibility to gene manipulation. Industries are looking for new microbial strains in order to produce different enzymes to fulfil the current enzyme
The unknown #257 tested positive for the enzyme DNase. Lastly, Mannitol Salt Agar (MSA) was used to test for isolation and differentiation. The streaking technique used is streaking for isolation. The unknown #257 tested positive for mannitol fermentation which means the organism is
The effects of alcohol on Biological Membranes. Introduction In this experiment it will be analysed the damage alcohols can have on biological membranes. Membranes are made up of lipids and proteins. Membranes usually help maintain the balance in a cell as it holds all the cellular materials.
Introduction Cloning is the processes that are used in order to generate exact genetic makeup of a cell, tissue, or organism. The term clone refers to the copied material with the same genetic makeup of the original. According to the definition by National Genome Research Institute (NIH) cloning can be differentiated into three types, those are: 1. Gene cloning, which creates copies of genes or segments of DNA. 2.
Finally, the amplified DNA regions are compare using a gel. DNA Profiling
