Mutagen Essays

1. Why is gel purification important? What is it used for? Gel-purification is a procedure that yields DNA freed from impurities such as salts, free nucleotides and enzymes, suitable for downstream applications Gel purification is used to recover desired DNA fragments from agarose gels after electrophoretic separation. Also to turn the pure DNA into a plasmid vector 2. What buffer is the DNA suspended in, after pillow purification? Tris Acetate (TAE) buffer 3. How is TOPO cloning different from

First part of this experiment was to create and plan out a dilution series to figure out the concentration of Salmonella enterica in the original solution. Three plates were labeled 1,2, and 3. Plate 1 being the most diluted mixture and 3 being the least diluted mixture. These plates were examined to figure out the CFU. The dilution factors of the samples were 1:30,000 for plate 1, the second plate was 1:60,000, and the third and final plate was 1:120,000. 100uL of each sample was pipetted onto the

This shows that there is no definite outcome of recombinational repair or mitotic crossing over caused by the mutagens, tartrazine and carmoisine. If tartrazine and carmoisine causes mitotic crossing over, then the homoallelic ade2 loci: ade2-40/ade2-40 would lead to an obstruction to the adenine pathway which then results in the formation of red colonies. However

the amount of colonies than the negative control but it was expected that the colony count to be higher. The TA1538 does not show it is mutagenic because a possible source error can be the bacteria labels were switched. The highest response was mutagen 1, which was Skittles. Skittles had 18 colonies, which is the highest number of colonies for TA1535 salmonella strain. This strain had 2x more than the negative control at 9 colonies. Which leads the Skittles to be mutagenic because it produced

Abstract: Cultures of the yeast, Saccharomyces cerevisiae, were irradiated with ultraviolet light. The purpose of the experiment was to determine the effect of ultraviolet light on wild type and mutant type S. cerevisiae growth and viability. Using four types of dilutions; 10-2 cells/mL, 10-3 cells/mL, 10-4 cells/ml, and 10-5 cells/ml of yeast, we irradiated them for 0, 30, 60 and 120 seconds. We then counted the yeast colonies within the petri plates using a grid technique. The number of yeast

In this experiment we tested to see if the catfish contained lead by using a lead testing kit. We used the data collected by ORNL in 2011 that is now accessible to the public. We pose that, if the age of the lake is older than the amount of lead will increase in the catfish when the amount of lead in the fish is a function of how old the lake is. Assuming the age of the lake would affect the amount of lead in the fish. The lakes that we picked were Tellico Lake, Melton Hill Lake, Watts Bar Lake

The distressing inquiry “am I beautiful?” generates 228,300,000 more Google results than “am I intelligent?” and “am I a good person?” combined. Inevitably, people always search for traces of their superiority over others－counting among them, beauty. The unhealthy tendency to place an emphasis on exterior appearances over inner beauty has ceaselessly saturated our society, from the ancient Greek kallisteia beauty contests (Calame 123) to more modern Miss America pageants. America’s first beauty

Mutation will not generate a new species in microevolution. Mutation is a change in nucleotide sequence of the genome of an organism, or chromosomal DNA or other genetic elements. Mutation occur when old alleles is changed into new alleles. This could occurred at two levels either in genetic levels or chromosomal levels. Since all cells in our body contain genetic materials, there are lots of places for mutations to occur. Somatic mutations occur in non-reproductive cells and will not be passed onto

How humans have come into existence has been a debateable topic for many years. It has been pondered upon and there have been thousans of theories stated to prove our history. The endless process that has been going on for years is what some people may call evolution. Physical evolution has been significantly influenced by genetic drift, fire, mutation, natural and sexual selection in the past 7 million years. These factors would be the reason we are who we are today. Genetic drift and fire is one

A Fluoridated Debate Have you ever thought about what you ingest in your body? Think about the numerous variations of chemicals added to “cleanse” your water. Is your drinking water truly safe, or is some deep conspiracy linked to water you drink every day. One of these said chemicals is fluoride. Most water plants all over the United States use at least one part per million of fluoride in tap water. It is supposed to help developing children have healthier teeth but there is significant debate to

Marijuana smoke is an irritant to the lungs as it depresses the lung causes respiratory diseases higher risk of having lung cancer compare with tobacco smokers (National Institute on Drug Abuse 2013). Marijuana smoke contains the same amount of mutagens and carcinogens that are also found in tobacco smoke (Svakic.D, Lustman, Mallaya, Lynn, Finney, Svrakic.N

PRODUCTION OF ANTIBODIES IN DUCK EGGS AGAINST LUNG CANCER Lung cancer was chosen since majority of lung cancers is caused by cigarette smoke which contains harmful chemicals that can cause gene mutations which lead to cancer. Smoking causes the majority of lung cancers — both in smokers and in people exposed to second-hand smoke. But lung cancer also occurs in people who never smoked. Smoking causes lung cancer by damaging the cells that line the lungs. When you inhale cigarette smoke, which is full

Katie Houser Silent Spring: AP Environmental Science Summer Assignment When marine biologist Rachel Carson released her ground-breaking book, "Silent Spring," in 1962, she signaled in a new awareness of how nature and man are interconnected. In the book, she detailed her observations about the effects of DDT—a chlorinated hydrocarbon invented in 1939 by Paul Müller, used originally to reduce mosquito populations and prevent the spread of lice during World War II, then eventually used extensively

Throughout history, the beach has been one of the mainstream vacation destinations across the globe. People from all over the world travel to experience the natural beauty that is the ocean. But more and more tourists are starting to realize that the amount of marine life in coal rees has decreased significantly. Currently, it’s like an underwater desert. Coral bleaching has been around for a long time. However, it is more in the recent years the the issue has become so impactful. Now is the time

Animal testing has been occurring forever. It can even comeback to the ancient times of the greeks and romans. Doctors would dissect animals with the interest to gain knowledge. But even back then, one practicing doctor stated that he would rather use a pig as his subject because he wanted to, “ avoid seeing the unpleasant expression of an ape” (Monamy 9). The thought of it being right or wrong to use animals for testing has always been questioned. Humans do not want to think of animals as on the

What is Laboratory Safety? Laboratory safety is of paramount importance to environmental health and safety, which manages and responds to all issues and concerns surrounding chemical, biological, radiation, laser, chemical fume hood and centrifuge safety as well as biological safety cabinets, compressed gas cylinders, nanotechnology, emergency spill response, hazardous waste management and other standard operating procedures.

Title of Invention "AN IMPROVED PROCESS FOR THE PREPARATION OF CAROTENOIDS FROM ENCYSTED HAEMATOCOCCUS CELLS" Abstract An improved process for the preparation of carotenoids from Heamatococcus cells characterized in simple method of extraction of said caratonoids by using acidified aqueous solution, which comprises suspending encysted Heamatococcus cells in acidified aqueous solution having concentration in the range of 0.01 N to N at a temperature range of 8-90° C for a period of 30 seconds to 15

General Concerns Finally, as with any mining or intensive industry, hydraulic fracturing has the potential for noise and light pollution, and environmental degradation from the construction of the site itself and access roads into increasingly remote areas, amplified erosion from land clearing, and habitat disturbance through the means above or the accidental introduction of invasive species though equipment or soil movement (Rahm and Riha, 2012). Political, Economic, and Social Dimensions Political

Name: Paul Meyer PS ID #: 1305816 BIOL 3311 Fall 2014 Lab Section: 16254 Date: 10/10/14 TA Instructor Name: Tess Doumas Writing Assignment 3: “Bearded (Brd) gene encodes for multiplication and thickening of chaetae and sensilla by interfering with neurogenic pathway” Introduction An important aspect of research is finding an appropriate model to use for performing one’s experiment(s). For biology and genetics, Drosophila melanogaster, the common fruit fly, has proven to be a suitable subject

A top priority for most women is beauty, but is it more important than health? It has been shown that in America, about one-third of women get their hair done unnaturally. Hair treatments that affect one’s health in the most negative way are Brazilian Blowouts, Brasil cacau cadiveu, keratin complex smoothing therapy, express blowout, and Marcia teixeira which are all types of hair smoothing treatments with the Brazilian Blowout as the most common. In Oregon, Occupational Safety and Health Administration