Differential media allows for the differentiation between two similar micro-organisms through how the bacteria may handle certain compounds found in the media or the different reactions that may take place when the bacteria is exposed to the medium (3). Selective media on the other hand allow only certain microbes to grow. This is due to the plate containing a limited amount of nutrients, compounds and chemicals that will deter the growth of certain bacteria (3). Dyes, antimicrobial substances, salts, certain growth inhibitors and, antibiotics are also found on this type of medium (3). The differential and selective media mentioned in this lab are as follows:
The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not
The plate without the plasmid that contained ampicillin, and the LB broth did not grow because ampicillin is an antibiotic that impeded the growth of bacteria because it killed off. There was no glow because it didn’t contain the green fluorescent protein gene or any other of the components to make it glow. The plate that contained the +pGLO, the ampicillin, and the LB broth was able to grow, because the plasmid provided the ampicillin resistance gene and the LB broth to feed the bacteria, however it wasn't able to glow because no arabinose was present to activate the GFP gene. Another plate that was able to grow was the one without the plasmid and just the LB broth. Finally the plate with all the components, +pGLO, arabinose, ampicillin, and broth was able to both grow and glow because the arabinose (sugar) turned the GFP gene on, the resistance to ampicillin gene was present and that let the bacteria survive and feed on the LB broth which let the culture
Winnable Battle- Safe Food Objectives: Approximately 1 of 6 people in America get sick from eating contaminated food. Some people recover and few others may suffer from complication such as kidney failure, miscarriage or brain and nerve damage (10 Colorado winnable battles). In Colorado, there is at least 41% of foodborne outbreaks report and investigation each year. This is an enormous number that underscores the need for vigilance and highlights the importance of the food safety programs to identify foodborne illnesses (10 Colorado winnable battles).
The purpose of this experiment was to insert the plasmid glow green into the bacteria with a gene of interest to produce the protein that make the bacteria glow green along with the presence of arabinose and the presence of ampicillin. Many scientists are experimenting different kind of genes that can inserted into the organism for survival. The technique of transformation was used in this experiment to give the organism a new trait that they did not possess in their life. In this experiment, the bacteria were added to four plates with certain conditions such as the existence of plasmid, ampicillin, and arabinose to see whether the bacteria grow and glow green. The results showed that the LB/amp/araC +pGLO produce a lot of colony and most
Introduction Our world is composed of many bacteria’s’ that can either help or destroy us. Therefore, its’s imperative to learn and study them. The purpose of the lab was to put into action the methods that have been learned in the laboratory to determine our unknown bacteria. Bacteria’s can have different features, shapes, and or arrangements that help microbiologist determined their role in our life (whether they are good or bad for humans).
Selective medium involves medium with environmental conditions that specifically grows some microbes while inhibiting others. Differential medium is used to identify and differentiate (as the title says) closely related microbes based on growth responses and physical indicators. It is imperative to use laboratory positive and negative controls in identifying the unknown because it confirms and compares the results of the unknown’s response to the definite guide. While performing the procedures in this report, students had to keep the bacterial and biological species concepts in context. The bacterial species concept is the identification and naming of microbes based on relating physical and physiological features of the unknown to the fitting taxa.
Our hypothesis was that if the plate contains only the LB broth the E. coli bacteria would have no antibiotic resistance and would not glow. If the plate contains just LB broth and ampicillin then the E. coli bacteria will have antibiotic resistance only if the plasmid is present. If the plate contains LB broth, ampicillin and arabinose then the E. coli bacteria will glow fluorescent under a UV light and it will have antibiotic resistance. Similar to our expectations our results suggested that our hypothesis was correct. This is due to the fact that n order for the E coli.
Food is the sustaining life force that drives the human race forwards from day to day. As daily consumers of food products, it is automatically expected that the producers of such important products aim to produce goods that will help the consumer and attribute to their health. Unfortunately, this is not always the case. At the turn of the twentieth century, food sanitation in factories was at an all time low. Adding to this issue were the harsh conditions in which the workers were forced to work in.
In the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates.
Being able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the
Bacteria that causes disease are called pathogens. The disease is caused by a poison called exotoxin and endotoxin produced by the bacteria. Another microbial life are protist. They are unicellular eukaryotes. Types of protist includes protozoans and slime molds.
Prokaryotic organisms normally have a cytoplasmic membrane, cell wall, and sometimes a capsule. Bacterial cells are most commonly either coccus or bacillus in shape. The cell wall is either Gram positive or Gram negative. When the cell is Gram negative, the cell has an extra layer of lipopolysaccharides. The Gram positive has a thick layer of peptidoglycan.
Food security is one of the greatest problems faced the world. There is fact said that food is enough for everyone in the world but because of the great changes which happened rapidly in the world the rate of food become less than before and it is difficult to secure it. Food security is very important to ensure that everyone has enough to eat and families can build their communities without worrying about securing their live. To meet global needs, food production must be doubled in the next years in order to solve many issues such as: starvation, malnutrition and associated health. According to The World Food Summit of 1996 defined food security as existing “when all people at all times have access to sufficient, safe, nutritious food to maintain
ABSTRACT Foodpanda, a food delivery services ranging from many different restaurants is something new and fresh in Brunei. It has received a warm welcomed in Brunei, with many customers excited to try the services through the website or mobile application. Foodpanda culture has the potential to be adapted in Brunei if the services are time saving, convenient to use and satisfying. At first, the services offered seem to be a good deal, but not anymore.
