Lab Report Sample

Abstract
Much of the biological study depends on specie identification, yet many of the taxonomic identifications are flawed. Many scientists are convinced that in order to create best identification is to use DNA barcoding. Few established genes that are globally accepted are rbcL and Matk in chloroplast for plants and Co1 mitochondrial DNA for animals. In order to prove this, we will perform a study where we receive an Unknown sample and by extracting the DNA from that unknown and creating more copies we will perform gel electrophoresis where we will sequencing the gene of the unknown and identify the sample using phylogenetics, Further more we will identify the taxonomy. The study concludes by refuting our original hypothesis of unknown

Genomic DNA was extracted based on Cold Spring Harbor DNA isolation kit (Carolina and Dolan Learning Center. Cold spring Harbo Laboratory). Sample was placed in 1.5 ml tube and nucleic lyses solution was added to the tube. Sample and lyses solution was forcefully grinded together for 1 minute. Sample then was incubated in heat bath of 65 C for 10 minutes. Sample was mixed for 4 minutes. Supernatant was collected from top of the sample and was mixed with of silica gel inside a tube. Sample then was incubated at 57C for 15 minutes. Sample was mixed for 30 seconds. Supernatant was removed following the mixing. Ice-cold wash buffer was added and mixed well. Superintendent was removed again. Second round of ice-cold wash was added to the pellet and mixed (Corolina 2014). The supernatant was removed again, leaving the pellet behind. Distilled water was added to the tube and pellet was mixed. Sample was incubated in water bath of 57C for 5 minutes. The supernatant was collected at the end and stored at

The unknown sample was small and many plants in nature have very similar looks. Based on these theories, DNA barcoding became popular. In order to identify the specific species this study was performed. The first thing that was required to perform was to extract DNA from the unknown sample. This process would not have been possible without the functions of lyses solution which dissolved the membrane bound organelles and by changing temperature of the sample, DNA was bound to the silica resin. At this time wash buffers were used to remove any unwanted contaminants and get the DNA ready for PCR. After completing the PCR many copies of the gene were made and were run through agarose electrophoresis. The PCR gel of purified and unpurified DNA concluded; that genetic material was successfully extracted and by purification, many unwanted material were disposed off. Purified DNA was then used for forward and reverse reactions for Sanger Sequencing. Completed sequencing reaction then was analyzed and a sequence of 612 bp was obtained. This data was used to determine the identity of the unknown sample and phylogenic tree was created. Also purified DNA was used for molecular cloning. The DNA was transformed into E.coli and incubated. Successful transformation, color white, were then cultured and analyzed with restriction enzymes. Before plasmids could have been introduced to