Microorganisms are tiny organisms that have been present on life from the past few billions of years. These tiny organisms are vital for the existence of human mankind and exist everywhere, in and out of the human body. Microbes include bacteria, fungi, protists, viruses and archae (1). Microbes however have indispensible advantages and benefits in many different areas. One example includes the industry of food production, where microbes hold a immense responsibility both on small scale, inside homes and on big scale too. For example, bacteria (lactic acid) can be used to make fermented milk products such as yogurt, sour cream, cheese etc. Also, Wine and beer can be made by using yeast, and vinegar by bacterial acetic acid fermentation. The other areas of benefits of the microbes include the medical field as many microbes are used as a source of vaccines and antibiotics, also they can be used to fight against other bad microbes. Microbes are also used in wastewater treatment and in agriculture too. Despite the advantages of microbes, there are microbes that are pathogenic and cause disease. Diseases that are …show more content…
The results show the presence of primer dimers for campylobacter sp in cetrimide agar, nutrient agar and blood agar and primer dimers for pseudomonas sp in all 4 agars. However, none of the bacteria’s had prominent bands on the gel after PCR, which possibly means that the DNA template concentration was too small as normally the concentration should be 20ng per 50 µl, and hence did not show bands (6). Or it could mean that there was insufficient template in the reaction, which could lead to insufficient amplification (7). The optimum range for these bacteria on this allele ladder is at 500-700bp however the primer dimers where present at 100-200bp as there were no prominent bands that could indicate the presence of the bacteria (refer to figure 3 and