Pglo Transformation Lab Report

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In this lab we did bacterial transformation with E.coli. We performed procedures to transform bacteria into green fluorescent protein (GFP). The purpose of this lab was to find the different effects of transformation with E.coli bacteria. We spread negative and positive pGLO into four different plates to observe the different effects of each one using heat shock and incubation. We observed that only the positive pGLO plates had countable growth on them. Bacterial transformation happens when bacteria uses foreign DNA and mixes it with its own DNA. This usually happen within plasmids. The purpose of this lab is to insert genes that will make E. coli resistant to ampicillin and to also glow. Our hypothesis for this lab experiment is that the

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